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Surface plasmon resonance-enabled mass spectrometry arrays.
Electrophoresis. 2006 Sep; 27(18):3671-5.E

Abstract

Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to beta-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.

Authors+Show Affiliations

Nedelkov D
Intrinsic Bioprobes Inc., Tempe, AZ 85281, USA. dnedelkov@intrinsicbio.com
Tubbs KA
No affiliation info available
Nelson RW
No affiliation info available

MeSH

AntibodiesBiosensing TechniquesBlood ProteinsHumansMass SpectrometryProtein Array AnalysisReproducibility of ResultsSensitivity and SpecificitySurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16915566

Citation

Nedelkov, Dobrin, et al. "Surface Plasmon Resonance-enabled Mass Spectrometry Arrays." Electrophoresis, vol. 27, no. 18, 2006, pp. 3671-5.
Nedelkov D, Tubbs KA, Nelson RW. Surface plasmon resonance-enabled mass spectrometry arrays. Electrophoresis. 2006;27(18):3671-5.
Nedelkov, D., Tubbs, K. A., & Nelson, R. W. (2006). Surface plasmon resonance-enabled mass spectrometry arrays. Electrophoresis, 27(18), 3671-5.
Nedelkov D, Tubbs KA, Nelson RW. Surface Plasmon Resonance-enabled Mass Spectrometry Arrays. Electrophoresis. 2006;27(18):3671-5. PubMed PMID: 16915566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance-enabled mass spectrometry arrays. AU - Nedelkov,Dobrin, AU - Tubbs,Kemmons A, AU - Nelson,Randall W, PY - 2006/8/18/pubmed PY - 2006/12/15/medline PY - 2006/8/18/entrez SP - 3671 EP - 5 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 18 N2 - Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to beta-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16915566/Surface_plasmon_resonance_enabled_mass_spectrometry_arrays_ L2 - https://doi.org/10.1002/elps.200600065 DB - PRIME DP - Unbound Medicine ER -