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Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jan 01; 845(1):180-3.JC

Abstract

We describe here a simple, precise, and highly sensitive method for the simultaneous determination of methamphetamine (MA) and amphetamine (AM) in urine using a high performance liquid chromatography (HPLC) column-switching method. A PK-2A (Shodex) column was used for extraction and deproteinization, and a CAPCELL PAK SCX semi-micro, polymer-coated cation-exchange column was employed for separation. The urine sample was mixed with an equal volume of borate buffer (0.1M, pH 9.4), and then 100 microl of the mixture was injected into the HPLC column. The column was switched for 6 min, and then 10 min later detection was performed at 210 nm. Recovery yields of the MA and AM spiked in the urine were 93.0-100.4% with a coefficient of variation of less than 1%. The calibration curves of MA and AM were in the range of 0.1-10 microg/ml with good linearity (r(2)=0.999), with the limit of qualification being 0.005 microg/ml. This method of using HPLC with column-switching can be used for both qualification and quantification of MA and its metabolite, AM, in urine, especially in forensic cases.

Authors+Show Affiliations

Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. kumihash@mxi.netwave.or.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16916628

Citation

Kumihashi, Mitsuru, et al. "Simultaneous Determination of Methamphetamine and Its Metabolite, Amphetamine, in Urine Using a High Performance Liquid Chromatography Column-switching Method." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 845, no. 1, 2007, pp. 180-3.
Kumihashi M, Ameno K, Shibayama T, et al. Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;845(1):180-3.
Kumihashi, M., Ameno, K., Shibayama, T., Suga, K., Miyauchi, H., Jamal, M., Wang, W., Uekita, I., & Ijiri, I. (2007). Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 845(1), 180-3.
Kumihashi M, et al. Simultaneous Determination of Methamphetamine and Its Metabolite, Amphetamine, in Urine Using a High Performance Liquid Chromatography Column-switching Method. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jan 1;845(1):180-3. PubMed PMID: 16916628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method. AU - Kumihashi,Mitsuru, AU - Ameno,Kiyoshi, AU - Shibayama,Takayuki, AU - Suga,Keisuke, AU - Miyauchi,Hiroshi, AU - Jamal,Mostofa, AU - Wang,Weihuan, AU - Uekita,Ikuo, AU - Ijiri,Iwao, Y1 - 2006/08/17/ PY - 2006/03/15/received PY - 2006/07/04/revised PY - 2006/07/27/accepted PY - 2006/8/19/pubmed PY - 2007/3/21/medline PY - 2006/8/19/entrez SP - 180 EP - 3 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 845 IS - 1 N2 - We describe here a simple, precise, and highly sensitive method for the simultaneous determination of methamphetamine (MA) and amphetamine (AM) in urine using a high performance liquid chromatography (HPLC) column-switching method. A PK-2A (Shodex) column was used for extraction and deproteinization, and a CAPCELL PAK SCX semi-micro, polymer-coated cation-exchange column was employed for separation. The urine sample was mixed with an equal volume of borate buffer (0.1M, pH 9.4), and then 100 microl of the mixture was injected into the HPLC column. The column was switched for 6 min, and then 10 min later detection was performed at 210 nm. Recovery yields of the MA and AM spiked in the urine were 93.0-100.4% with a coefficient of variation of less than 1%. The calibration curves of MA and AM were in the range of 0.1-10 microg/ml with good linearity (r(2)=0.999), with the limit of qualification being 0.005 microg/ml. This method of using HPLC with column-switching can be used for both qualification and quantification of MA and its metabolite, AM, in urine, especially in forensic cases. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16916628/Simultaneous_determination_of_methamphetamine_and_its_metabolite_amphetamine_in_urine_using_a_high_performance_liquid_chromatography_column_switching_method_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00644-1 DB - PRIME DP - Unbound Medicine ER -