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Control of ColE1 plasmid replication. Intermediates in the binding of RNA I and RNA II.
J Mol Biol. 1990 Apr 20; 212(4):683-94.JM

Abstract

Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions forming complexes differing in the stability. Formation of a very unstable early intermediate that was previously inferred from the inhibition of stable binding caused by a second RNA I species was firmly established by more extensive studies. This complex is converted to a more stable yet reversible complex that was identified by its RNase sensitivity, which was altered from that of the earlier complex or from that of free RNA I or RNA II. In these complexes, most loops of RNA II interact with their complementary loops of RNA I. The kinetic and structural analyses of the binding process predict formation of a complex interacting at a single pair of complementary loops that precedes formation of these complexes. Thus the process of binding of RNA I to RNA II is seen to consist of a sequence of reactions producing a series of progressively more stable intermediates leading to the final product.

Authors+Show Affiliations

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1691790

Citation

Tomizawa, J. "Control of ColE1 Plasmid Replication. Intermediates in the Binding of RNA I and RNA II." Journal of Molecular Biology, vol. 212, no. 4, 1990, pp. 683-94.
Tomizawa J. Control of ColE1 plasmid replication. Intermediates in the binding of RNA I and RNA II. J Mol Biol. 1990;212(4):683-94.
Tomizawa, J. (1990). Control of ColE1 plasmid replication. Intermediates in the binding of RNA I and RNA II. Journal of Molecular Biology, 212(4), 683-94.
Tomizawa J. Control of ColE1 Plasmid Replication. Intermediates in the Binding of RNA I and RNA II. J Mol Biol. 1990 Apr 20;212(4):683-94. PubMed PMID: 1691790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of ColE1 plasmid replication. Intermediates in the binding of RNA I and RNA II. A1 - Tomizawa,J, PY - 1990/4/20/pubmed PY - 1990/4/20/medline PY - 1990/4/20/entrez SP - 683 EP - 94 JF - Journal of molecular biology JO - J. Mol. Biol. VL - 212 IS - 4 N2 - Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions forming complexes differing in the stability. Formation of a very unstable early intermediate that was previously inferred from the inhibition of stable binding caused by a second RNA I species was firmly established by more extensive studies. This complex is converted to a more stable yet reversible complex that was identified by its RNase sensitivity, which was altered from that of the earlier complex or from that of free RNA I or RNA II. In these complexes, most loops of RNA II interact with their complementary loops of RNA I. The kinetic and structural analyses of the binding process predict formation of a complex interacting at a single pair of complementary loops that precedes formation of these complexes. Thus the process of binding of RNA I to RNA II is seen to consist of a sequence of reactions producing a series of progressively more stable intermediates leading to the final product. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/1691790/Control_of_ColE1_plasmid_replication__Intermediates_in_the_binding_of_RNA_I_and_RNA_II_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0022-2836(90)90230-J DB - PRIME DP - Unbound Medicine ER -