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Induction of atopic eczema/dermatitis syndrome-like skin lesions by repeated topical application of a crude extract of Dermatophagoides pteronyssinus in NC/Nga mice.
Int Immunopharmacol. 2006 Oct; 6(10):1616-22.II

Abstract

Mite antigen has been considered to play important roles in the development of atopic eczema/dermatitis syndrome (AEDS). In the present study, we attempted to induce an AEDS-like skin lesion in mice using Dermatophagoides pteronyssinus crude extract (DPE) as an antigen and performed pathophysiological evaluations. Ears of mice were tape-stripped and DPE was painted 3 times a week. Eczematous skin lesion and ear swelling were apparent in NC/Nga mice treated with DPE after 2 weeks, whereas neither skin lesion nor ear swelling were observed in BALB/c mice even after 30 days. Histological evaluation demonstrated that edema, epidermal hyperplasia and the accumulation of inflammatory cells were apparent in the ears of DPE-treated NC/Nga mice. In contrast to skin lesion and ear swelling, total serum IgE levels were increased in both NC/Nga and BALB/c mice. Treatment with DPE also increased auricular lymph node weight in both NC/Nga mice and BALB/c mice. To further characterize, we analyzed cytokine mRNA expression in ears and lymph nodes of DPE-treated NC/Nga mice. Increased expression of IL-4 and TNF-alpha mRNA was observed in both ears and lymph nodes of NC/Nga mice treated with DPE. Additionally, there was no change in the responsiveness of BALB/c mice to DPE treatment by adaptive transfer of serum from DPE-treated NC/Nga mice to BALB/c mice. Taken together, our results indicate that eczematous skin lesion and ear swelling caused by repeated application of DPE in NC/Nga mice has a Th2-dominant background and that inflammation is involved in this process. The animal model of AEDS established in this report may be used to investigate the pathogenesis of AEDS and evaluate the potential therapeutic agents for AEDS.

Authors+Show Affiliations

Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology, Taejon,305-333, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16919834

Citation

Kang, Jong Soon, et al. "Induction of Atopic Eczema/dermatitis Syndrome-like Skin Lesions By Repeated Topical Application of a Crude Extract of Dermatophagoides Pteronyssinus in NC/Nga Mice." International Immunopharmacology, vol. 6, no. 10, 2006, pp. 1616-22.
Kang JS, Lee K, Han SB, et al. Induction of atopic eczema/dermatitis syndrome-like skin lesions by repeated topical application of a crude extract of Dermatophagoides pteronyssinus in NC/Nga mice. Int Immunopharmacol. 2006;6(10):1616-22.
Kang, J. S., Lee, K., Han, S. B., Ahn, J. M., Lee, H., Han, M. H., Yoon, Y. D., Yoon, W. K., Park, S. K., & Kim, H. M. (2006). Induction of atopic eczema/dermatitis syndrome-like skin lesions by repeated topical application of a crude extract of Dermatophagoides pteronyssinus in NC/Nga mice. International Immunopharmacology, 6(10), 1616-22.
Kang JS, et al. Induction of Atopic Eczema/dermatitis Syndrome-like Skin Lesions By Repeated Topical Application of a Crude Extract of Dermatophagoides Pteronyssinus in NC/Nga Mice. Int Immunopharmacol. 2006;6(10):1616-22. PubMed PMID: 16919834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of atopic eczema/dermatitis syndrome-like skin lesions by repeated topical application of a crude extract of Dermatophagoides pteronyssinus in NC/Nga mice. AU - Kang,Jong Soon, AU - Lee,Kiho, AU - Han,Sang-Bae, AU - Ahn,Ji-Mi, AU - Lee,Hyunju, AU - Han,Mi Hwa, AU - Yoon,Yeo Dae, AU - Yoon,Won Kee, AU - Park,Song-Kyu, AU - Kim,Hwan Mook, Y1 - 2006/07/18/ PY - 2006/03/30/received PY - 2006/06/12/revised PY - 2006/06/15/accepted PY - 2006/8/22/pubmed PY - 2006/10/14/medline PY - 2006/8/22/entrez SP - 1616 EP - 22 JF - International immunopharmacology JO - Int Immunopharmacol VL - 6 IS - 10 N2 - Mite antigen has been considered to play important roles in the development of atopic eczema/dermatitis syndrome (AEDS). In the present study, we attempted to induce an AEDS-like skin lesion in mice using Dermatophagoides pteronyssinus crude extract (DPE) as an antigen and performed pathophysiological evaluations. Ears of mice were tape-stripped and DPE was painted 3 times a week. Eczematous skin lesion and ear swelling were apparent in NC/Nga mice treated with DPE after 2 weeks, whereas neither skin lesion nor ear swelling were observed in BALB/c mice even after 30 days. Histological evaluation demonstrated that edema, epidermal hyperplasia and the accumulation of inflammatory cells were apparent in the ears of DPE-treated NC/Nga mice. In contrast to skin lesion and ear swelling, total serum IgE levels were increased in both NC/Nga and BALB/c mice. Treatment with DPE also increased auricular lymph node weight in both NC/Nga mice and BALB/c mice. To further characterize, we analyzed cytokine mRNA expression in ears and lymph nodes of DPE-treated NC/Nga mice. Increased expression of IL-4 and TNF-alpha mRNA was observed in both ears and lymph nodes of NC/Nga mice treated with DPE. Additionally, there was no change in the responsiveness of BALB/c mice to DPE treatment by adaptive transfer of serum from DPE-treated NC/Nga mice to BALB/c mice. Taken together, our results indicate that eczematous skin lesion and ear swelling caused by repeated application of DPE in NC/Nga mice has a Th2-dominant background and that inflammation is involved in this process. The animal model of AEDS established in this report may be used to investigate the pathogenesis of AEDS and evaluate the potential therapeutic agents for AEDS. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/16919834/Induction_of_atopic_eczema/dermatitis_syndrome_like_skin_lesions_by_repeated_topical_application_of_a_crude_extract_of_Dermatophagoides_pteronyssinus_in_NC/Nga_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(06)00179-2 DB - PRIME DP - Unbound Medicine ER -