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In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria.
Anaerobe 2006; 12(4):173-7A

Abstract

The aim was to evaluate the effect of 30% ethanolic extract of Bulgarian propolis on 94 clinical anaerobic strains. The strains were tested by both agar-well diffusion (wells, 7 mm diameter) and disk-diffusion methods. Only 15% of Clostridium-, 3.3% of other Gram-positive- and 9.1% of Gram-negative anaerobic strains were not inhibited by 30 microL propolis extract per well. Propolis extract was more active than the ethanol (P < 0.001). By 30 microL extract per well, mean inhibitory diameters of the clostridia, other Gram-positive- and Gram-negative anaerobes were 11.5, 13.1, and 11.3 mm, and those by 90 microL were 16, 18.1 and 15.4 mm, respectively. Mean inhibitory diameters of all strains by 30 and 90 microL ethanol were only 8.4 and 9.5 mm. By 30 microL propolis extract per well, inhibitory diameters of 15 mm or more were more common in Gram-positive (32%) than in Gram-negative bacteria (13.6%, P < 0.05). Moist propolis disks inhibited more strains (89.4%) than dried disks (68.1%, P < 0.001). Most (81.8%) Bacteroides fragilis group strains and 75% of clostridial strains were inhibited by moist EEP disks.

CONCLUSION

Bulgarian propolis was active against most anaerobic strains of different genera. In addition to oral pathogens, an activity of propolis against Clostridium, Bacteroides and Propionibacterium species was observed. The results could motivate a higher medical interest and further trials for evaluating the use of bee glue for prophylaxis or treatment of some anaerobic infections such as oral, skin and wound diseases.

Authors+Show Affiliations

Department of Microbiology, Medical University of Sofia, Zdrave Street 2, 1431 Sofia, Bulgaria. lboyanova@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16919977

Citation

Boyanova, Lyudmila, et al. "In Vitro Activity of Bulgarian Propolis Against 94 Clinical Isolates of Anaerobic Bacteria." Anaerobe, vol. 12, no. 4, 2006, pp. 173-7.
Boyanova L, Kolarov R, Gergova G, et al. In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. Anaerobe. 2006;12(4):173-7.
Boyanova, L., Kolarov, R., Gergova, G., & Mitov, I. (2006). In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. Anaerobe, 12(4), pp. 173-7.
Boyanova L, et al. In Vitro Activity of Bulgarian Propolis Against 94 Clinical Isolates of Anaerobic Bacteria. Anaerobe. 2006;12(4):173-7. PubMed PMID: 16919977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. AU - Boyanova,Lyudmila, AU - Kolarov,Rossen, AU - Gergova,Galina, AU - Mitov,Ivan, Y1 - 2006/08/21/ PY - 2006/04/27/received PY - 2006/06/07/revised PY - 2006/06/16/accepted PY - 2006/8/22/pubmed PY - 2006/12/9/medline PY - 2006/8/22/entrez SP - 173 EP - 7 JF - Anaerobe JO - Anaerobe VL - 12 IS - 4 N2 - UNLABELLED: The aim was to evaluate the effect of 30% ethanolic extract of Bulgarian propolis on 94 clinical anaerobic strains. The strains were tested by both agar-well diffusion (wells, 7 mm diameter) and disk-diffusion methods. Only 15% of Clostridium-, 3.3% of other Gram-positive- and 9.1% of Gram-negative anaerobic strains were not inhibited by 30 microL propolis extract per well. Propolis extract was more active than the ethanol (P < 0.001). By 30 microL extract per well, mean inhibitory diameters of the clostridia, other Gram-positive- and Gram-negative anaerobes were 11.5, 13.1, and 11.3 mm, and those by 90 microL were 16, 18.1 and 15.4 mm, respectively. Mean inhibitory diameters of all strains by 30 and 90 microL ethanol were only 8.4 and 9.5 mm. By 30 microL propolis extract per well, inhibitory diameters of 15 mm or more were more common in Gram-positive (32%) than in Gram-negative bacteria (13.6%, P < 0.05). Moist propolis disks inhibited more strains (89.4%) than dried disks (68.1%, P < 0.001). Most (81.8%) Bacteroides fragilis group strains and 75% of clostridial strains were inhibited by moist EEP disks. CONCLUSION: Bulgarian propolis was active against most anaerobic strains of different genera. In addition to oral pathogens, an activity of propolis against Clostridium, Bacteroides and Propionibacterium species was observed. The results could motivate a higher medical interest and further trials for evaluating the use of bee glue for prophylaxis or treatment of some anaerobic infections such as oral, skin and wound diseases. SN - 1075-9964 UR - https://www.unboundmedicine.com/medline/citation/16919977/In_vitro_activity_of_Bulgarian_propolis_against_94_clinical_isolates_of_anaerobic_bacteria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(06)00046-1 DB - PRIME DP - Unbound Medicine ER -