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Determination of tamsulosin in human aqueous humor and serum by liquid chromatography-electrospray ionization tandem mass spectrometry.
J Pharm Biomed Anal. 2007 Jan 17; 43(2):606-12.JP

Abstract

A simple, sensitive and selective LC-MS/MS method was developed for the determination of tamsulosin in human aqueous humor and serum to study the recently reported eye-related adverse effects of this alpha(1)-blocker drug. Aqueous humor samples were analyzed by direct injection, after addition of the internal standard, labetalol. Liquid-liquid extraction with ethyl acetate was used for serum sample preparation. The chromatographic separation was performed on a reversed phase column by gradient elution with acetonitrile -0.1% formic acid at a flow-rate of 0.2 ml/min. Detection and quantification of the analytes were carried out with a linear ion trap mass spectrometer, using positive electrospray ionization (ESI) and multiple reaction monitoring (MRM). The limit of quantification was 0.1 ng/ml for both aqueous humor and serum samples and linearity was obtained over the concentration ranges of 0.1-4.7 ng/ml and 0.1-19.3 ng/ml for aqueous humor and serum samples, respectively. Acceptable accuracy and precision were obtained for concentrations within the standard curve ranges. The method has been used for the determination of tamsulosin in aqueous humor and serum samples from patients that were on tamsulosin medication and underwent cataract surgery.

Authors+Show Affiliations

Department of Pharmaceutical Chemistry, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. pekka.keski-rahkonen@uku.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Validation Study

Language

eng

PubMed ID

16920322

Citation

Keski-Rahkonen, Pekka, et al. "Determination of Tamsulosin in Human Aqueous Humor and Serum By Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 43, no. 2, 2007, pp. 606-12.
Keski-Rahkonen P, Pärssinen O, Leppänen E, et al. Determination of tamsulosin in human aqueous humor and serum by liquid chromatography-electrospray ionization tandem mass spectrometry. J Pharm Biomed Anal. 2007;43(2):606-12.
Keski-Rahkonen, P., Pärssinen, O., Leppänen, E., Mauriala, T., Lehtonen, M., & Auriola, S. (2007). Determination of tamsulosin in human aqueous humor and serum by liquid chromatography-electrospray ionization tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 43(2), 606-12.
Keski-Rahkonen P, et al. Determination of Tamsulosin in Human Aqueous Humor and Serum By Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry. J Pharm Biomed Anal. 2007 Jan 17;43(2):606-12. PubMed PMID: 16920322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of tamsulosin in human aqueous humor and serum by liquid chromatography-electrospray ionization tandem mass spectrometry. AU - Keski-Rahkonen,Pekka, AU - Pärssinen,Olavi, AU - Leppänen,Esa, AU - Mauriala,Timo, AU - Lehtonen,Marko, AU - Auriola,Seppo, Y1 - 2006/08/22/ PY - 2006/04/10/received PY - 2006/07/07/accepted PY - 2006/8/22/pubmed PY - 2007/7/10/medline PY - 2006/8/22/entrez SP - 606 EP - 12 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 43 IS - 2 N2 - A simple, sensitive and selective LC-MS/MS method was developed for the determination of tamsulosin in human aqueous humor and serum to study the recently reported eye-related adverse effects of this alpha(1)-blocker drug. Aqueous humor samples were analyzed by direct injection, after addition of the internal standard, labetalol. Liquid-liquid extraction with ethyl acetate was used for serum sample preparation. The chromatographic separation was performed on a reversed phase column by gradient elution with acetonitrile -0.1% formic acid at a flow-rate of 0.2 ml/min. Detection and quantification of the analytes were carried out with a linear ion trap mass spectrometer, using positive electrospray ionization (ESI) and multiple reaction monitoring (MRM). The limit of quantification was 0.1 ng/ml for both aqueous humor and serum samples and linearity was obtained over the concentration ranges of 0.1-4.7 ng/ml and 0.1-19.3 ng/ml for aqueous humor and serum samples, respectively. Acceptable accuracy and precision were obtained for concentrations within the standard curve ranges. The method has been used for the determination of tamsulosin in aqueous humor and serum samples from patients that were on tamsulosin medication and underwent cataract surgery. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16920322/Determination_of_tamsulosin_in_human_aqueous_humor_and_serum_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(06)00468-7 DB - PRIME DP - Unbound Medicine ER -