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Muramyl dipeptide induces production of hemopoietic growth factors in vivo by a mechanism independent of tumor necrosis factor.
J Immunol. 1990 May 15; 144(10):3789-94.JI

Abstract

Monocyte products including TNF and IL-1 can stimulate hemopoietic growth factor production in vitro and in vivo. Endotoxin-resistant C3H/HeJ mice have a cellular defect in the regulation of TNF production and provide a model in which to examine the relative importance of TNF and IL-1. To assess the relative role of TNF and IL-1, we injected the synthetic bacterial cell wall derivative muramyl dipeptide (MDP) or LPS into C3H/HeJ mice. Both MDP and LPS stimulated hemopoietic growth factor production in a dose-dependent manner. Northern blot analysis of splenic and lung mRNA revealed that MDP treatment induced granulocyte-macrophage CSF and macrophage-CSF transcripts, whereas LPS treatment induced macrophage-CSF transcripts. Messenger RNA for granulocyte-CSF, IL-3, IL-4, and IL-5 was not detected in control or treated mice. MDP treatment induced IL-1 mRNA but not TNF mRNA, and TNF bioactivity was not detected in the serum of MDP-treated mice. In contrast, LPS treatment induced TNF production. These results identify the hemopoietic growth factors induced by MDP and LPS in vivo, and suggest that MDP stimulates growth factor production by a mechanism independent of TNF.

Authors+Show Affiliations

Department of Medicine, University of Washington, Seattle 98195.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1692060

Citation

Broudy, V C., et al. "Muramyl Dipeptide Induces Production of Hemopoietic Growth Factors in Vivo By a Mechanism Independent of Tumor Necrosis Factor." Journal of Immunology (Baltimore, Md. : 1950), vol. 144, no. 10, 1990, pp. 3789-94.
Broudy VC, Kaushansky K, Shoemaker SG, et al. Muramyl dipeptide induces production of hemopoietic growth factors in vivo by a mechanism independent of tumor necrosis factor. J Immunol. 1990;144(10):3789-94.
Broudy, V. C., Kaushansky, K., Shoemaker, S. G., Aggarwal, B. B., & Adamson, J. W. (1990). Muramyl dipeptide induces production of hemopoietic growth factors in vivo by a mechanism independent of tumor necrosis factor. Journal of Immunology (Baltimore, Md. : 1950), 144(10), 3789-94.
Broudy VC, et al. Muramyl Dipeptide Induces Production of Hemopoietic Growth Factors in Vivo By a Mechanism Independent of Tumor Necrosis Factor. J Immunol. 1990 May 15;144(10):3789-94. PubMed PMID: 1692060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Muramyl dipeptide induces production of hemopoietic growth factors in vivo by a mechanism independent of tumor necrosis factor. AU - Broudy,V C, AU - Kaushansky,K, AU - Shoemaker,S G, AU - Aggarwal,B B, AU - Adamson,J W, PY - 1990/5/15/pubmed PY - 1990/5/15/medline PY - 1990/5/15/entrez SP - 3789 EP - 94 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 144 IS - 10 N2 - Monocyte products including TNF and IL-1 can stimulate hemopoietic growth factor production in vitro and in vivo. Endotoxin-resistant C3H/HeJ mice have a cellular defect in the regulation of TNF production and provide a model in which to examine the relative importance of TNF and IL-1. To assess the relative role of TNF and IL-1, we injected the synthetic bacterial cell wall derivative muramyl dipeptide (MDP) or LPS into C3H/HeJ mice. Both MDP and LPS stimulated hemopoietic growth factor production in a dose-dependent manner. Northern blot analysis of splenic and lung mRNA revealed that MDP treatment induced granulocyte-macrophage CSF and macrophage-CSF transcripts, whereas LPS treatment induced macrophage-CSF transcripts. Messenger RNA for granulocyte-CSF, IL-3, IL-4, and IL-5 was not detected in control or treated mice. MDP treatment induced IL-1 mRNA but not TNF mRNA, and TNF bioactivity was not detected in the serum of MDP-treated mice. In contrast, LPS treatment induced TNF production. These results identify the hemopoietic growth factors induced by MDP and LPS in vivo, and suggest that MDP stimulates growth factor production by a mechanism independent of TNF. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1692060/Muramyl_dipeptide_induces_production_of_hemopoietic_growth_factors_in_vivo_by_a_mechanism_independent_of_tumor_necrosis_factor_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1692060 DB - PRIME DP - Unbound Medicine ER -