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Comparison of antioxidant activities between salvianolic acid B and Ginkgo biloba extract (EGb 761).
Acta Pharmacol Sin. 2006 Sep; 27(9):1137-45.AP

Abstract

AIM

To investigate and compare the antioxidant activities of salvianolic acid B (SalB) and Ginkgo biloba extract (EGb 761) in aqueous solution, rat microsomes and the cellular system.

METHODS

Superoxide anion (O2-.) was generated using xanthine/xanthine oxidase system and phenazine methosulate/NADH system, and the effects of SalB and EGb 761 on the generation of O2-.were achieved by spectrophotometric measurement of the product formed on reduction of nitro blue tetrazolium. Two different methods were used to assess the scavenging effects of the extracts on hydroxyl radical (. OH): HPLC method was used for quantitation of . OH by oxy-radical trapping of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) to form DMPO-OH adducts in Fe2+-EDTA-H2O2 system. To confirm the HPLC data, .OH was also measured by spectrophotometry using a commercial detection kit. The anti-lipid peroxidation effects of the extracts in microsomes of rat brain, liver and kidney induced by ascorbate-NADPH were determined by thiobarbituric acid (TBA) method. The protective effects of the extracts on peroxide hydrogen (H2O2)-induced oxidative damage in SH-SY5Y cells were investigated by assessing cell viability assay, the level of lipid peroxidation, and the lactate dehydrogenase(LDH) release.

RESULTS

Both SalB and EGb 761 were able to scavenge O2-. and . OH, inhibit lipid peroxidation of microsomes, and protect SH-SY5Y cells against H2O2-induced oxidative damage. However, the concentration of SalB was far lower than that of EGb 761 when a similar effect was obtained.

CONCLUSION

The antioxidant efficiency of SalB was greater than that of EGb 761. These results suggest that SalB, like EGb 761, has promising potential in treating oxidative damage-derived neurodegenerative disorders.

Authors+Show Affiliations

Department of Neuropharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16923333

Citation

Liu, Chang-suo, et al. "Comparison of Antioxidant Activities Between Salvianolic Acid B and Ginkgo Biloba Extract (EGb 761)." Acta Pharmacologica Sinica, vol. 27, no. 9, 2006, pp. 1137-45.
Liu CS, Cheng Y, Hu JF, et al. Comparison of antioxidant activities between salvianolic acid B and Ginkgo biloba extract (EGb 761). Acta Pharmacol Sin. 2006;27(9):1137-45.
Liu, C. S., Cheng, Y., Hu, J. F., Zhang, W., Chen, N. H., & Zhang, J. T. (2006). Comparison of antioxidant activities between salvianolic acid B and Ginkgo biloba extract (EGb 761). Acta Pharmacologica Sinica, 27(9), 1137-45.
Liu CS, et al. Comparison of Antioxidant Activities Between Salvianolic Acid B and Ginkgo Biloba Extract (EGb 761). Acta Pharmacol Sin. 2006;27(9):1137-45. PubMed PMID: 16923333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of antioxidant activities between salvianolic acid B and Ginkgo biloba extract (EGb 761). AU - Liu,Chang-suo, AU - Cheng,Yong, AU - Hu,Jin-feng, AU - Zhang,Wei, AU - Chen,Nai-hong, AU - Zhang,Jun-tian, PY - 2006/8/23/pubmed PY - 2007/10/20/medline PY - 2006/8/23/entrez SP - 1137 EP - 45 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 27 IS - 9 N2 - AIM: To investigate and compare the antioxidant activities of salvianolic acid B (SalB) and Ginkgo biloba extract (EGb 761) in aqueous solution, rat microsomes and the cellular system. METHODS: Superoxide anion (O2-.) was generated using xanthine/xanthine oxidase system and phenazine methosulate/NADH system, and the effects of SalB and EGb 761 on the generation of O2-.were achieved by spectrophotometric measurement of the product formed on reduction of nitro blue tetrazolium. Two different methods were used to assess the scavenging effects of the extracts on hydroxyl radical (. OH): HPLC method was used for quantitation of . OH by oxy-radical trapping of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) to form DMPO-OH adducts in Fe2+-EDTA-H2O2 system. To confirm the HPLC data, .OH was also measured by spectrophotometry using a commercial detection kit. The anti-lipid peroxidation effects of the extracts in microsomes of rat brain, liver and kidney induced by ascorbate-NADPH were determined by thiobarbituric acid (TBA) method. The protective effects of the extracts on peroxide hydrogen (H2O2)-induced oxidative damage in SH-SY5Y cells were investigated by assessing cell viability assay, the level of lipid peroxidation, and the lactate dehydrogenase(LDH) release. RESULTS: Both SalB and EGb 761 were able to scavenge O2-. and . OH, inhibit lipid peroxidation of microsomes, and protect SH-SY5Y cells against H2O2-induced oxidative damage. However, the concentration of SalB was far lower than that of EGb 761 when a similar effect was obtained. CONCLUSION: The antioxidant efficiency of SalB was greater than that of EGb 761. These results suggest that SalB, like EGb 761, has promising potential in treating oxidative damage-derived neurodegenerative disorders. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/16923333/Comparison_of_antioxidant_activities_between_salvianolic_acid_B_and_Ginkgo_biloba_extract__EGb_761__ L2 - https://doi.org/10.1111/j.1745-7254.2006.00378.x DB - PRIME DP - Unbound Medicine ER -