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Bacillus subtilis aconitase is required for efficient late-sporulation gene expression.
J Bacteriol. 2006 Sep; 188(17):6396-405.JB

Abstract

Bacillus subtilis aconitase, encoded by the citB gene, is homologous to the bifunctional eukaryotic protein IRP-1 (iron regulatory protein 1). Like IRP-1, B. subtilis aconitase is both an enzyme and an RNA binding protein. In an attempt to separate the two activities of aconitase, the C-terminal region of the B. subtilis citB gene product was mutagenized. The resulting strain had high catalytic activity but was defective in sporulation. The defect was at a late stage of sporulation, specifically affecting expression of sigmaK-dependent genes, many of which are important for spore coat assembly and require transcriptional activation by GerE. Accumulation of gerE mRNA and GerE protein was delayed in the aconitase mutant strain. Pure B. subtilis aconitase bound to the 3' untranslated region of gerE mRNA in in vitro gel mobility shift assays, strongly suggesting that aconitase RNA binding activity may stabilize gerE mRNA in order to allow efficient GerE synthesis and proper timing of spore coat assembly.

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Authors+Show Affiliations

Serio AW
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.
Pechter KB
No affiliation info available
Sonenshein AL
No affiliation info available

MeSH

Aconitate HydrataseAmino Acid SequenceBacillus subtilisBacterial ProteinsGene Expression Regulation, BacterialMolecular Sequence DataPoint MutationRNA, MessengerRNA-Binding ProteinsSequence AlignmentSpores, BacterialTranscription Factors

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16923907

Citation

Serio, Alisa W., et al. "Bacillus Subtilis Aconitase Is Required for Efficient Late-sporulation Gene Expression." Journal of Bacteriology, vol. 188, no. 17, 2006, pp. 6396-405.
Serio AW, Pechter KB, Sonenshein AL. Bacillus subtilis aconitase is required for efficient late-sporulation gene expression. J Bacteriol. 2006;188(17):6396-405.
Serio, A. W., Pechter, K. B., & Sonenshein, A. L. (2006). Bacillus subtilis aconitase is required for efficient late-sporulation gene expression. Journal of Bacteriology, 188(17), 6396-405.
Serio AW, Pechter KB, Sonenshein AL. Bacillus Subtilis Aconitase Is Required for Efficient Late-sporulation Gene Expression. J Bacteriol. 2006;188(17):6396-405. PubMed PMID: 16923907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacillus subtilis aconitase is required for efficient late-sporulation gene expression. AU - Serio,Alisa W, AU - Pechter,Kieran B, AU - Sonenshein,Abraham L, PY - 2006/8/23/pubmed PY - 2006/10/3/medline PY - 2006/8/23/entrez SP - 6396 EP - 405 JF - Journal of bacteriology JO - J Bacteriol VL - 188 IS - 17 N2 - Bacillus subtilis aconitase, encoded by the citB gene, is homologous to the bifunctional eukaryotic protein IRP-1 (iron regulatory protein 1). Like IRP-1, B. subtilis aconitase is both an enzyme and an RNA binding protein. In an attempt to separate the two activities of aconitase, the C-terminal region of the B. subtilis citB gene product was mutagenized. The resulting strain had high catalytic activity but was defective in sporulation. The defect was at a late stage of sporulation, specifically affecting expression of sigmaK-dependent genes, many of which are important for spore coat assembly and require transcriptional activation by GerE. Accumulation of gerE mRNA and GerE protein was delayed in the aconitase mutant strain. Pure B. subtilis aconitase bound to the 3' untranslated region of gerE mRNA in in vitro gel mobility shift assays, strongly suggesting that aconitase RNA binding activity may stabilize gerE mRNA in order to allow efficient GerE synthesis and proper timing of spore coat assembly. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/16923907/Bacillus_subtilis_aconitase_is_required_for_efficient_late_sporulation_gene_expression_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=16923907 DB - PRIME DP - Unbound Medicine ER -