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Toxicological and cytotoxic evaluation of standardized extracts of Galphimia glauca.
J Ethnopharmacol 2007; 109(1):35-40JE

Abstract

Galphimia glauca Cav (Malpighiaceae) has been widely used in Mexican traditional medicine as a remedy for the treatment of mental disorders, principally as a sedative and tranquilizer. The sedative activity of extracts obtained from this plant has been demonstrated with different neuropharmacological models. Different triterpenes, known as galphimines, have been identified from the active extract. Galphimine-B (G-B) possesses anxiolytic activity and is able to selectively inhibit discharges of dopaminergic neurons in the ventral tegmental area in rats. Nevertheless, there have been no toxicological investigations carried out with products obtained from this plant. In this work three different extracts (aqueous, ethanolic, and methanolic) of Galphimia glauca, standardized in the content of three galphimines, were evaluated for their behavioral and pharmaco-toxicological effects. After administering the extracts to mice for 28 days (2.5g/kg, p.o.), no deaths were found and the histopathological analysis of different organs did not show alterations; only the behavioral parameters analyzed showed a diminution of spontaneous activity. The administration of these extracts for 56 days (same doses and route) in mice did not cause any changes in the biochemical parameters that evaluate liver function. On the other hand, no cytotoxic effects were found on KB, UISO, and OVCAR-5 transformed cell lines, but all extracts specifically inhibited colon cancer cell line growth with an ED(50) lower than 2microg/ml. The extracts were also evaluated in genotoxicity tests in vitro (250, 100 and 50microg/ml), which demonstrate that none of the three extracts from Galphimia glauca showed a genotoxic effect.

Authors+Show Affiliations

Centro de Investigación Biomédica del Sur, IMSS, Argentina 1, Xochitepec, Morelos CP 62790, México.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16930894

Citation

Aguilar-Santamaría, Lucía, et al. "Toxicological and Cytotoxic Evaluation of Standardized Extracts of Galphimia Glauca." Journal of Ethnopharmacology, vol. 109, no. 1, 2007, pp. 35-40.
Aguilar-Santamaría L, Ramírez G, Herrera-Arellano A, et al. Toxicological and cytotoxic evaluation of standardized extracts of Galphimia glauca. J Ethnopharmacol. 2007;109(1):35-40.
Aguilar-Santamaría, L., Ramírez, G., Herrera-Arellano, A., Zamilpa, A., Jiménez, J. E., Alonso-Cortés, D., ... Tortoriello, J. (2007). Toxicological and cytotoxic evaluation of standardized extracts of Galphimia glauca. Journal of Ethnopharmacology, 109(1), pp. 35-40.
Aguilar-Santamaría L, et al. Toxicological and Cytotoxic Evaluation of Standardized Extracts of Galphimia Glauca. J Ethnopharmacol. 2007 Jan 3;109(1):35-40. PubMed PMID: 16930894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicological and cytotoxic evaluation of standardized extracts of Galphimia glauca. AU - Aguilar-Santamaría,Lucía, AU - Ramírez,Guillermo, AU - Herrera-Arellano,Armando, AU - Zamilpa,Alejandro, AU - Jiménez,Jesús E, AU - Alonso-Cortés,Daniel, AU - Cortés-Gutiérrez,Elva I, AU - Ledesma,Nestor, AU - Tortoriello,Jaime, Y1 - 2006/07/08/ PY - 2005/11/07/received PY - 2006/06/22/revised PY - 2006/06/30/accepted PY - 2006/8/26/pubmed PY - 2007/4/14/medline PY - 2006/8/26/entrez SP - 35 EP - 40 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 109 IS - 1 N2 - Galphimia glauca Cav (Malpighiaceae) has been widely used in Mexican traditional medicine as a remedy for the treatment of mental disorders, principally as a sedative and tranquilizer. The sedative activity of extracts obtained from this plant has been demonstrated with different neuropharmacological models. Different triterpenes, known as galphimines, have been identified from the active extract. Galphimine-B (G-B) possesses anxiolytic activity and is able to selectively inhibit discharges of dopaminergic neurons in the ventral tegmental area in rats. Nevertheless, there have been no toxicological investigations carried out with products obtained from this plant. In this work three different extracts (aqueous, ethanolic, and methanolic) of Galphimia glauca, standardized in the content of three galphimines, were evaluated for their behavioral and pharmaco-toxicological effects. After administering the extracts to mice for 28 days (2.5g/kg, p.o.), no deaths were found and the histopathological analysis of different organs did not show alterations; only the behavioral parameters analyzed showed a diminution of spontaneous activity. The administration of these extracts for 56 days (same doses and route) in mice did not cause any changes in the biochemical parameters that evaluate liver function. On the other hand, no cytotoxic effects were found on KB, UISO, and OVCAR-5 transformed cell lines, but all extracts specifically inhibited colon cancer cell line growth with an ED(50) lower than 2microg/ml. The extracts were also evaluated in genotoxicity tests in vitro (250, 100 and 50microg/ml), which demonstrate that none of the three extracts from Galphimia glauca showed a genotoxic effect. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/16930894/Toxicological_and_cytotoxic_evaluation_of_standardized_extracts_of_Galphimia_glauca_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(06)00327-8 DB - PRIME DP - Unbound Medicine ER -