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Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.).
Theor Appl Genet. 2006 Nov; 113(7):1271-81.TA

Abstract

The density of SSRs on the published genetic map of bread wheat (Triticum aestivum L.) has steadily increased over the last few years. This has improved the efficiency of marker-assisted breeding and certain types of genetic research by providing more choice in the quality of SSRs and a greater chance of finding polymorphic markers in any cross for a chromosomal region of interest. Increased SSR density on the published wheat genetic map will further enhance breeding and research efforts. Here, sequence-tagged microsatellite profiling (STMP) is demonstrated as a rapid technique for the economical development of anonymous genomic SSRs to increase marker density on the wheat genetic map. A total of 684 polymorphic sequence-tagged microsatellites (STMs) were developed, and 380 were genetically mapped in three mapping populations, with 296 being mapped in the International Triticeae Mapping Initiative W7984 x Opata85 recombinant inbred cross. Across the three populations, a total of 479 STM loci were mapped. Several technological advantages of STMs over conventional SSRs were also observed. These include reduced marker deployment costs for fluorescent-based SSR analysis, and increased genotyping throughput by more efficient electrophoretic separation of STMs and a high amenability to multiplex PCR.

Authors+Show Affiliations

Hayden MJ
Plant Functional Genomics Center, University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia. matthew.hayden@adelaide.edu.au
Stephenson P
No affiliation info available
Logojan AM
No affiliation info available
Khatkar D
No affiliation info available
Rogers C
No affiliation info available
Elsden J
No affiliation info available
Koebner RM
No affiliation info available
Snape JW
No affiliation info available
Sharp PJ
No affiliation info available

MeSH

Chromosome MappingDNA PrimersMicrosatellite RepeatsPolymorphism, GeneticSequence Tagged SitesTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16932882

Citation

Hayden, M J., et al. "Development and Genetic Mapping of Sequence-tagged Microsatellites (STMs) in Bread Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 113, no. 7, 2006, pp. 1271-81.
Hayden MJ, Stephenson P, Logojan AM, et al. Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2006;113(7):1271-81.
Hayden, M. J., Stephenson, P., Logojan, A. M., Khatkar, D., Rogers, C., Elsden, J., Koebner, R. M., Snape, J. W., & Sharp, P. J. (2006). Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 113(7), 1271-81.
Hayden MJ, et al. Development and Genetic Mapping of Sequence-tagged Microsatellites (STMs) in Bread Wheat (Triticum Aestivum L.). Theor Appl Genet. 2006;113(7):1271-81. PubMed PMID: 16932882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.). AU - Hayden,M J, AU - Stephenson,P, AU - Logojan,A M, AU - Khatkar,D, AU - Rogers,C, AU - Elsden,J, AU - Koebner,R M D, AU - Snape,J W, AU - Sharp,P J, Y1 - 2006/08/25/ PY - 2006/01/11/received PY - 2006/07/25/accepted PY - 2006/8/26/pubmed PY - 2007/1/25/medline PY - 2006/8/26/entrez SP - 1271 EP - 81 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 113 IS - 7 N2 - The density of SSRs on the published genetic map of bread wheat (Triticum aestivum L.) has steadily increased over the last few years. This has improved the efficiency of marker-assisted breeding and certain types of genetic research by providing more choice in the quality of SSRs and a greater chance of finding polymorphic markers in any cross for a chromosomal region of interest. Increased SSR density on the published wheat genetic map will further enhance breeding and research efforts. Here, sequence-tagged microsatellite profiling (STMP) is demonstrated as a rapid technique for the economical development of anonymous genomic SSRs to increase marker density on the wheat genetic map. A total of 684 polymorphic sequence-tagged microsatellites (STMs) were developed, and 380 were genetically mapped in three mapping populations, with 296 being mapped in the International Triticeae Mapping Initiative W7984 x Opata85 recombinant inbred cross. Across the three populations, a total of 479 STM loci were mapped. Several technological advantages of STMs over conventional SSRs were also observed. These include reduced marker deployment costs for fluorescent-based SSR analysis, and increased genotyping throughput by more efficient electrophoretic separation of STMs and a high amenability to multiplex PCR. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16932882/Development_and_genetic_mapping_of_sequence_tagged_microsatellites__STMs__in_bread_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-006-0381-4 DB - PRIME DP - Unbound Medicine ER -