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[Construction of Escherichia coli-Bifidobacterium longum shuttle vector and expression of tumor suppressor gene PTEN in B. longum].
Wei Sheng Wu Xue Bao. 2006 Jun; 46(3):347-52.WS

Abstract

It was reported that Bifidobacterium longum accumulated specifically in hypoxic solid tumors, therefore could be used as a delivery system for cancer-specific gene therapy. Furthermore, construction of E.coli-B. longum shuttle vectors was proved by other research to be an efficient way for stable gene expression in B. longum. To obtain a shuttle vector and analyze the inhibition on mice solid tumors by genetically engineered B. longum, 48 primers with mutual overlaps were designed, assisted by software package Oligo 6.0. By PCR with the above primers, a linear plasmid was synthesized, which consists of pMB1 and HU gene promoter, both from B. longum. pMB-HU was constructed by cloning the synthesized linear plasmid into E.coli vector pMD 18-T, and was proved to be stably replicated in both E.coli DH5alpha and B. longum L17. By inserting PTEN cDNA into pMB-HU, expression vector pMB-HU-PTEN was obtained, in which PTEN gene was reported as a major tumor suppressor gene encoding a dual-specificity phosphatase. pMB-HU-PTEN was then transferred into B. longum L17 by electroporation. After transformation, an effective expression of PTEN in B. longum L17 was confirmed by Western blot, and significant inhibition on growth of mice solid tumors was also observed with the above genetically engineered B. longum. Those obtained results have laid foundation for tumor-targeting gene therapy with B. longum.

Authors+Show Affiliations

Hou X
College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. houxin2005@263.net
Liu JE
No affiliation info available

MeSH

AnimalsBifidobacteriumBlotting, WesternEscherichia coliGene ExpressionGenetic TherapyGenetic VectorsLiver NeoplasmsMicePTEN PhosphohydrolasePlasmidsTransformation, Bacterial

Pub Type(s)

Journal Article

Language

chi

PubMed ID

16933599

Citation

Hou, Xin, and Jun-E Liu. "[Construction of Escherichia coli-Bifidobacterium Longum Shuttle Vector and Expression of Tumor Suppressor Gene PTEN in B. Longum]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 46, no. 3, 2006, pp. 347-52.
Hou X, Liu JE. [Construction of Escherichia coli-Bifidobacterium longum shuttle vector and expression of tumor suppressor gene PTEN in B. longum]. Wei Sheng Wu Xue Bao. 2006;46(3):347-52.
Hou, X., & Liu, J. E. (2006). [Construction of Escherichia coli-Bifidobacterium longum shuttle vector and expression of tumor suppressor gene PTEN in B. longum]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 46(3), 347-52.
Hou X, Liu JE. [Construction of Escherichia coli-Bifidobacterium Longum Shuttle Vector and Expression of Tumor Suppressor Gene PTEN in B. Longum]. Wei Sheng Wu Xue Bao. 2006;46(3):347-52. PubMed PMID: 16933599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction of Escherichia coli-Bifidobacterium longum shuttle vector and expression of tumor suppressor gene PTEN in B. longum]. AU - Hou,Xin, AU - Liu,Jun-E, PY - 2006/8/29/pubmed PY - 2009/5/2/medline PY - 2006/8/29/entrez SP - 347 EP - 52 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 46 IS - 3 N2 - It was reported that Bifidobacterium longum accumulated specifically in hypoxic solid tumors, therefore could be used as a delivery system for cancer-specific gene therapy. Furthermore, construction of E.coli-B. longum shuttle vectors was proved by other research to be an efficient way for stable gene expression in B. longum. To obtain a shuttle vector and analyze the inhibition on mice solid tumors by genetically engineered B. longum, 48 primers with mutual overlaps were designed, assisted by software package Oligo 6.0. By PCR with the above primers, a linear plasmid was synthesized, which consists of pMB1 and HU gene promoter, both from B. longum. pMB-HU was constructed by cloning the synthesized linear plasmid into E.coli vector pMD 18-T, and was proved to be stably replicated in both E.coli DH5alpha and B. longum L17. By inserting PTEN cDNA into pMB-HU, expression vector pMB-HU-PTEN was obtained, in which PTEN gene was reported as a major tumor suppressor gene encoding a dual-specificity phosphatase. pMB-HU-PTEN was then transferred into B. longum L17 by electroporation. After transformation, an effective expression of PTEN in B. longum L17 was confirmed by Western blot, and significant inhibition on growth of mice solid tumors was also observed with the above genetically engineered B. longum. Those obtained results have laid foundation for tumor-targeting gene therapy with B. longum. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/16933599/[Construction_of_Escherichia_coli_Bifidobacterium_longum_shuttle_vector_and_expression_of_tumor_suppressor_gene_PTEN_in_B__longum]_ L2 - https://antibodies.cancer.gov/detail/CPTC-PTEN-1 DB - PRIME DP - Unbound Medicine ER -