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Silica directly increases permeability of alveolar epithelial cells.
J Appl Physiol (1985) 1990; 68(4):1354-9JA

Abstract

Alveolar epithelial cell injury and increased alveolar-capillary membrane permeability are important features of acute silicosis. To determine whether silica particles contribute directly to this increased permeability, we measured paracellular permeability of rat alveolar epithelium after exposure to silica, in vitro, using markers of the extracellular space. Silica (Minusil) markedly increased permeability in a dose- and time-dependent manner. This was not the result of cytolytic injury, because lactate dehydrogenase release from monolayers exposed to silica was not increased. Pretreatment of the silica with serum, charged dextrans, or aluminum sulfate blocked the increase in permeability. Scanning electron microscopy demonstrated adherence of the silica to the surface of the alveolar epithelial cells. Thus silica can directly increase permeability of alveolar epithelium.

Authors+Show Affiliations

Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1693364

Citation

Merchant, R K., et al. "Silica Directly Increases Permeability of Alveolar Epithelial Cells." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 68, no. 4, 1990, pp. 1354-9.
Merchant RK, Peterson MW, Hunninghake GW. Silica directly increases permeability of alveolar epithelial cells. J Appl Physiol. 1990;68(4):1354-9.
Merchant, R. K., Peterson, M. W., & Hunninghake, G. W. (1990). Silica directly increases permeability of alveolar epithelial cells. Journal of Applied Physiology (Bethesda, Md. : 1985), 68(4), pp. 1354-9.
Merchant RK, Peterson MW, Hunninghake GW. Silica Directly Increases Permeability of Alveolar Epithelial Cells. J Appl Physiol. 1990;68(4):1354-9. PubMed PMID: 1693364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silica directly increases permeability of alveolar epithelial cells. AU - Merchant,R K, AU - Peterson,M W, AU - Hunninghake,G W, PY - 1990/4/1/pubmed PY - 1990/4/1/medline PY - 1990/4/1/entrez SP - 1354 EP - 9 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J. Appl. Physiol. VL - 68 IS - 4 N2 - Alveolar epithelial cell injury and increased alveolar-capillary membrane permeability are important features of acute silicosis. To determine whether silica particles contribute directly to this increased permeability, we measured paracellular permeability of rat alveolar epithelium after exposure to silica, in vitro, using markers of the extracellular space. Silica (Minusil) markedly increased permeability in a dose- and time-dependent manner. This was not the result of cytolytic injury, because lactate dehydrogenase release from monolayers exposed to silica was not increased. Pretreatment of the silica with serum, charged dextrans, or aluminum sulfate blocked the increase in permeability. Scanning electron microscopy demonstrated adherence of the silica to the surface of the alveolar epithelial cells. Thus silica can directly increase permeability of alveolar epithelium. SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/1693364/Silica_directly_increases_permeability_of_alveolar_epithelial_cells_ L2 - http://www.physiology.org/doi/full/10.1152/jappl.1990.68.4.1354?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -