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Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons.
Brain Res 2006; 1115(1):9-15BR

Abstract

In the present study, ATP-activated currents (I(ATP)s) recorded from rat trigeminal ganglion (TG) neurons using whole-cell patch clamp technique are classified into three types (F, I and S) based on the characteristics of their activation and desensitization. The time of rising phase (R(10-90)) of types F, I and S of I(ATP) is measured to be 33.6+/-4.5, 62.2+/-9.9 and 302.1+/-62.0 ms respectively, and positively correlated to cell size. The time of decaying phases (D(10-90)) of types F and S is 399.4+/-58.2 and >1500 ms, respectively. The dose-response curves for the three types of I(ATP) show that their EC(50) values are close (3.44 x 10(-5), 4.89 x 10(-5) and 4.14 x 10(-5) M for types F, I and S respectively, P>0.05). Their reversal potentials are basically the same, varying from +4 to +10 mV. In addition, using whole-cell patch clamp technique in combination with single cell immunohistochemical staining for P2X receptor subunits, our results suggest that the type distinction of ATP-activated current was associated with cell size and P2X receptor subunits: small-sized cells with type F of I(ATP) express only P2X1 and/or P2X3 subunits, while cells with types S and I of I(ATP) express P2X2 or P2X4 in addition to P2X1 and P2X3.

Authors+Show Affiliations

Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16934235

Citation

Luo, Jialie, et al. "Characterization of Three Types of ATP-activated Current in Relation to P2X Subunits in Rat Trigeminal Ganglion Neurons." Brain Research, vol. 1115, no. 1, 2006, pp. 9-15.
Luo J, Yin GF, Gu YZ, et al. Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons. Brain Res. 2006;1115(1):9-15.
Luo, J., Yin, G. F., Gu, Y. Z., Liu, Y., Dai, J. P., Li, C., & Li, Z. W. (2006). Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons. Brain Research, 1115(1), pp. 9-15.
Luo J, et al. Characterization of Three Types of ATP-activated Current in Relation to P2X Subunits in Rat Trigeminal Ganglion Neurons. Brain Res. 2006 Oct 18;1115(1):9-15. PubMed PMID: 16934235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons. AU - Luo,Jialie, AU - Yin,Guang-Fu, AU - Gu,Yuan-Zheng, AU - Liu,Yang, AU - Dai,Jia-Pei, AU - Li,Chaoying, AU - Li,Zhi-Wang, Y1 - 2006/08/24/ PY - 2005/11/17/received PY - 2006/07/13/revised PY - 2006/07/24/accepted PY - 2006/8/29/pubmed PY - 2006/12/9/medline PY - 2006/8/29/entrez SP - 9 EP - 15 JF - Brain research JO - Brain Res. VL - 1115 IS - 1 N2 - In the present study, ATP-activated currents (I(ATP)s) recorded from rat trigeminal ganglion (TG) neurons using whole-cell patch clamp technique are classified into three types (F, I and S) based on the characteristics of their activation and desensitization. The time of rising phase (R(10-90)) of types F, I and S of I(ATP) is measured to be 33.6+/-4.5, 62.2+/-9.9 and 302.1+/-62.0 ms respectively, and positively correlated to cell size. The time of decaying phases (D(10-90)) of types F and S is 399.4+/-58.2 and >1500 ms, respectively. The dose-response curves for the three types of I(ATP) show that their EC(50) values are close (3.44 x 10(-5), 4.89 x 10(-5) and 4.14 x 10(-5) M for types F, I and S respectively, P>0.05). Their reversal potentials are basically the same, varying from +4 to +10 mV. In addition, using whole-cell patch clamp technique in combination with single cell immunohistochemical staining for P2X receptor subunits, our results suggest that the type distinction of ATP-activated current was associated with cell size and P2X receptor subunits: small-sized cells with type F of I(ATP) express only P2X1 and/or P2X3 subunits, while cells with types S and I of I(ATP) express P2X2 or P2X4 in addition to P2X1 and P2X3. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/16934235/Characterization_of_three_types_of_ATP_activated_current_in_relation_to_P2X_subunits_in_rat_trigeminal_ganglion_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(06)02220-7 DB - PRIME DP - Unbound Medicine ER -