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Lactobacillus paracasei A survives gastrointestinal passage and affects the fecal microbiota of healthy infants.
Res Microbiol. 2006 Nov; 157(9):857-66.RM

Abstract

This study focuses on the potentiality of a putative probiotic strain, Lactobacillus paracasei A, to survive gastrointestinal (GI) passage and modulate the resident microbiota of healthy infants. In a placebo-controlled study, 26 children aged 12-24 months received 100 g/day of either fermented milk containing strain A or pasteurized yogurt for four weeks. Fecal samples were analyzed before starting the administration, after 1, 3 and 4 weeks of consumption and after washout. The fate of strain A was followed by means of a newly developed PCR targeting a strain-specific genomic marker. The composition and dynamics of fecal microbial communities during the study were analyzed by culturing on selective media and by the PCR-denaturing gradient gel electrophoresis (DGGE) technique using universal and group-specific (Lactobacillus and Bifidobacterium) primers. The variation in enzymatic activities in infant feces during probiotic consumption was also analyzed. Strain A survived in fecal samples in most (92%) of the infants examined after 1 week of consumption, and temporarily dominated the intestinal Lactobacillus community. The administration of L. paracasei A led to a significant increment in the Lactobacillus population, while a moderate effect upon the main bacterial groups in the GI ecosystem was observed. Strain A also affected the diversity of the Lactobacillus and Bifidobacterium populations. The fecal bacterial structure of 1 - 2-year-old infants seems to combine neonate and adult-like features. The microbiota of these subjects promptly responded to probiotic consumption, later restoring the endogenous equilibrium.

Authors+Show Affiliations

Department of Science and Technology, University of Verona, Strada Le Grazie, 15, 37134 Verona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

16934438

Citation

Marzotto, Marta, et al. "Lactobacillus Paracasei a Survives Gastrointestinal Passage and Affects the Fecal Microbiota of Healthy Infants." Research in Microbiology, vol. 157, no. 9, 2006, pp. 857-66.
Marzotto M, Maffeis C, Paternoster T, et al. Lactobacillus paracasei A survives gastrointestinal passage and affects the fecal microbiota of healthy infants. Res Microbiol. 2006;157(9):857-66.
Marzotto, M., Maffeis, C., Paternoster, T., Ferrario, R., Rizzotti, L., Pellegrino, M., Dellaglio, F., & Torriani, S. (2006). Lactobacillus paracasei A survives gastrointestinal passage and affects the fecal microbiota of healthy infants. Research in Microbiology, 157(9), 857-66.
Marzotto M, et al. Lactobacillus Paracasei a Survives Gastrointestinal Passage and Affects the Fecal Microbiota of Healthy Infants. Res Microbiol. 2006;157(9):857-66. PubMed PMID: 16934438.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lactobacillus paracasei A survives gastrointestinal passage and affects the fecal microbiota of healthy infants. AU - Marzotto,Marta, AU - Maffeis,Claudio, AU - Paternoster,Thomas, AU - Ferrario,Rossano, AU - Rizzotti,Lucia, AU - Pellegrino,Maristella, AU - Dellaglio,Franco, AU - Torriani,Sandra, Y1 - 2006/08/02/ PY - 2005/12/22/received PY - 2006/05/30/revised PY - 2006/06/28/accepted PY - 2006/8/29/pubmed PY - 2007/1/27/medline PY - 2006/8/29/entrez SP - 857 EP - 66 JF - Research in microbiology JO - Res Microbiol VL - 157 IS - 9 N2 - This study focuses on the potentiality of a putative probiotic strain, Lactobacillus paracasei A, to survive gastrointestinal (GI) passage and modulate the resident microbiota of healthy infants. In a placebo-controlled study, 26 children aged 12-24 months received 100 g/day of either fermented milk containing strain A or pasteurized yogurt for four weeks. Fecal samples were analyzed before starting the administration, after 1, 3 and 4 weeks of consumption and after washout. The fate of strain A was followed by means of a newly developed PCR targeting a strain-specific genomic marker. The composition and dynamics of fecal microbial communities during the study were analyzed by culturing on selective media and by the PCR-denaturing gradient gel electrophoresis (DGGE) technique using universal and group-specific (Lactobacillus and Bifidobacterium) primers. The variation in enzymatic activities in infant feces during probiotic consumption was also analyzed. Strain A survived in fecal samples in most (92%) of the infants examined after 1 week of consumption, and temporarily dominated the intestinal Lactobacillus community. The administration of L. paracasei A led to a significant increment in the Lactobacillus population, while a moderate effect upon the main bacterial groups in the GI ecosystem was observed. Strain A also affected the diversity of the Lactobacillus and Bifidobacterium populations. The fecal bacterial structure of 1 - 2-year-old infants seems to combine neonate and adult-like features. The microbiota of these subjects promptly responded to probiotic consumption, later restoring the endogenous equilibrium. SN - 0923-2508 UR - https://www.unboundmedicine.com/medline/citation/16934438/Lactobacillus_paracasei_A_survives_gastrointestinal_passage_and_affects_the_fecal_microbiota_of_healthy_infants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(06)00147-1 DB - PRIME DP - Unbound Medicine ER -