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Adaptation of a recombinant xylose-utilizing Saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors.
Bioresour Technol. 2007 Jul; 98(9):1767-73.BT

Abstract

Adaptation of a xylose-utilizing genetically engineered strain of Saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production. The remaining xylose concentration in the medium at the end of the cultivation was 5.2g l(-1), while it was 11gl(-1) in the feed, indicating that approximately half of the xylose was consumed. The performance of the adapted strain was compared with the parental strain with respect to its ability to ferment three bagasse hydrolysates with different inhibitor concentration. The ethanol yield after 24h of fermentation of the bagasse hydrolysate with lowest inhibitor concentration increased from 0.18gg(-1) of total sugar with the non-adapted strain to 0.38gg(-1) with the adapted strain. The specific ethanol productivity increased from 1.15g ethanol per g initial biomass per h with the non-adapted strain to 2.55gg(-1) h(-1) with the adapted strain. The adapted strain performed better than the non-adapted also in the two bagasse hydrolysates containing higher concentrations of inhibitors. The adapted strain converted the inhibitory furaldehydes 2-furaldehyde (furfural) and 5-hydroxymethyl-2-furaldehyde (HMF) at a faster rate than the non-adapted strain. The xylose-utilizing ability of the yeast strain did not seem to be affected by the adaptation and the results suggest that ethanol rather than xylitol was formed from the consumed xylose.

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Authors+Show Affiliations

Martín C
Applied Microbiology, Lund University/Lund Institute of Technology, P.O. Box 124, SE-22100 Lund, Sweden.
Marcet M
No affiliation info available
Almazán O
No affiliation info available
Jönsson LJ
No affiliation info available

MeSH

Adaptation, PhysiologicalBiomassCelluloseEthanolFatty AcidsFermentationFuraldehydeGenetic EngineeringHydrolysisKineticsPhenolsRecombinant ProteinsSaccharomyces cerevisiaeSaccharumXylose

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16934451

Citation

Martín, Carlos, et al. "Adaptation of a Recombinant Xylose-utilizing Saccharomyces Cerevisiae Strain to a Sugarcane Bagasse Hydrolysate With High Content of Fermentation Inhibitors." Bioresource Technology, vol. 98, no. 9, 2007, pp. 1767-73.
Martín C, Marcet M, Almazán O, et al. Adaptation of a recombinant xylose-utilizing Saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors. Bioresour Technol. 2007;98(9):1767-73.
Martín, C., Marcet, M., Almazán, O., & Jönsson, L. J. (2007). Adaptation of a recombinant xylose-utilizing Saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors. Bioresource Technology, 98(9), 1767-73.
Martín C, et al. Adaptation of a Recombinant Xylose-utilizing Saccharomyces Cerevisiae Strain to a Sugarcane Bagasse Hydrolysate With High Content of Fermentation Inhibitors. Bioresour Technol. 2007;98(9):1767-73. PubMed PMID: 16934451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adaptation of a recombinant xylose-utilizing Saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors. AU - Martín,Carlos, AU - Marcet,Marcelo, AU - Almazán,Oscar, AU - Jönsson,Leif J, Y1 - 2006/08/24/ PY - 2006/03/22/received PY - 2006/06/03/revised PY - 2006/07/03/accepted PY - 2006/8/29/pubmed PY - 2007/4/12/medline PY - 2006/8/29/entrez SP - 1767 EP - 73 JF - Bioresource technology JO - Bioresour Technol VL - 98 IS - 9 N2 - Adaptation of a xylose-utilizing genetically engineered strain of Saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production. The remaining xylose concentration in the medium at the end of the cultivation was 5.2g l(-1), while it was 11gl(-1) in the feed, indicating that approximately half of the xylose was consumed. The performance of the adapted strain was compared with the parental strain with respect to its ability to ferment three bagasse hydrolysates with different inhibitor concentration. The ethanol yield after 24h of fermentation of the bagasse hydrolysate with lowest inhibitor concentration increased from 0.18gg(-1) of total sugar with the non-adapted strain to 0.38gg(-1) with the adapted strain. The specific ethanol productivity increased from 1.15g ethanol per g initial biomass per h with the non-adapted strain to 2.55gg(-1) h(-1) with the adapted strain. The adapted strain performed better than the non-adapted also in the two bagasse hydrolysates containing higher concentrations of inhibitors. The adapted strain converted the inhibitory furaldehydes 2-furaldehyde (furfural) and 5-hydroxymethyl-2-furaldehyde (HMF) at a faster rate than the non-adapted strain. The xylose-utilizing ability of the yeast strain did not seem to be affected by the adaptation and the results suggest that ethanol rather than xylitol was formed from the consumed xylose. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/16934451/Adaptation_of_a_recombinant_xylose_utilizing_Saccharomyces_cerevisiae_strain_to_a_sugarcane_bagasse_hydrolysate_with_high_content_of_fermentation_inhibitors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(06)00349-X DB - PRIME DP - Unbound Medicine ER -