Tags

Type your tag names separated by a space and hit enter

Assessment of the DNA damaging potency and chemopreventive effects towards BaP-induced genotoxicity in human derived cells by Monimiastrum globosum, an endemic Mauritian plant.
Toxicol In Vitro. 2006 Dec; 20(8):1427-34.TV

Abstract

Naturally occurring compounds have protective effects towards mutagens and carcinogens. The leaf extract of Monimiastrum globosum (Bois de Clous), a Mauritian endemic plant from the Myrtaceae family, was studied for its potency to induce DNA damage in human HepG2 hepatoma cells using DNA migration as a biological endpoint in the alkaline single cell gel electrophoresis (SCGE) assay. This was contrasted with the ability to modulate the benzo[a]pyrene (BaP)-dependent DNA damage in human hepatoma cells. M. globosum caused genotoxicity in HepG2 cells at concentrations exceeding 3mg fresh weight (FW) per ml cell culture in the absence of cytotoxicity. Pre-treatment of the cells with 12.2 microg FW/ml to 1.56 mg FW/ml led to a pronounced antigenotoxic effect towards BaP-induced DNA damage. DNA migration (OTM) was reduced by 66%, 81.5% and 74% for 49, 98 and 195 microg FW/ml, respectively. A U-shaped dose-response curve was derived for M. globosum indicating genotoxic effects in high doses and antigenotoxic effects in low doses. M. globosum extract had total phenolics (15 mg/g FW) with flavonoids (aglycones and conjugates: 8 mg/g FW) and proanthocyanidins (3mg/g FW) as major phenolic subclasses. The hydrolysis of conjugated flavonoids yielded the aglycones quercetin (606 microg/g FW) and kaempferol (117.8 microg/g FW) while HPLC-MS/MS analysis of the total extract revealed free flavonoids such as quercetin (19.2 microg/g FW) and myricetin (2.5 microg/g FW). The antioxidant activity of the extract of M. globosum, assessed by the FRAP and TEAC assays yielded values of 275+/-3.82 micromol/g FW and 346+/-4.2 micromol/g FW, respectively.

Authors+Show Affiliations

Institute of Indoor and Environmental Toxicology, Faculty of Medicine, Justus-Liebig University of Giessen, Giessen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16934955

Citation

Mersch-Sundermann, Volker, et al. "Assessment of the DNA Damaging Potency and Chemopreventive Effects Towards BaP-induced Genotoxicity in Human Derived Cells By Monimiastrum Globosum, an Endemic Mauritian Plant." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 20, no. 8, 2006, pp. 1427-34.
Mersch-Sundermann V, Bahorun T, Stahl T, et al. Assessment of the DNA damaging potency and chemopreventive effects towards BaP-induced genotoxicity in human derived cells by Monimiastrum globosum, an endemic Mauritian plant. Toxicol In Vitro. 2006;20(8):1427-34.
Mersch-Sundermann, V., Bahorun, T., Stahl, T., Neergheen, V. S., Soobrattee, M. A., Wohlfarth, R., Sobel, R., Brunn, H. E., Schmeiser, T., Lamy, E., & Aruoma, O. I. (2006). Assessment of the DNA damaging potency and chemopreventive effects towards BaP-induced genotoxicity in human derived cells by Monimiastrum globosum, an endemic Mauritian plant. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 20(8), 1427-34.
Mersch-Sundermann V, et al. Assessment of the DNA Damaging Potency and Chemopreventive Effects Towards BaP-induced Genotoxicity in Human Derived Cells By Monimiastrum Globosum, an Endemic Mauritian Plant. Toxicol In Vitro. 2006;20(8):1427-34. PubMed PMID: 16934955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of the DNA damaging potency and chemopreventive effects towards BaP-induced genotoxicity in human derived cells by Monimiastrum globosum, an endemic Mauritian plant. AU - Mersch-Sundermann,Volker, AU - Bahorun,Theeshan, AU - Stahl,Thorsten, AU - Neergheen,Vidushi S, AU - Soobrattee,Muhammad A, AU - Wohlfarth,Robert, AU - Sobel,Rosa, AU - Brunn,Hubertus E, AU - Schmeiser,Tim, AU - Lamy,Evelyn, AU - Aruoma,Okezie I, Y1 - 2006/07/20/ PY - 2006/02/08/received PY - 2006/06/14/revised PY - 2006/06/26/accepted PY - 2006/8/29/pubmed PY - 2006/12/27/medline PY - 2006/8/29/entrez SP - 1427 EP - 34 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 20 IS - 8 N2 - Naturally occurring compounds have protective effects towards mutagens and carcinogens. The leaf extract of Monimiastrum globosum (Bois de Clous), a Mauritian endemic plant from the Myrtaceae family, was studied for its potency to induce DNA damage in human HepG2 hepatoma cells using DNA migration as a biological endpoint in the alkaline single cell gel electrophoresis (SCGE) assay. This was contrasted with the ability to modulate the benzo[a]pyrene (BaP)-dependent DNA damage in human hepatoma cells. M. globosum caused genotoxicity in HepG2 cells at concentrations exceeding 3mg fresh weight (FW) per ml cell culture in the absence of cytotoxicity. Pre-treatment of the cells with 12.2 microg FW/ml to 1.56 mg FW/ml led to a pronounced antigenotoxic effect towards BaP-induced DNA damage. DNA migration (OTM) was reduced by 66%, 81.5% and 74% for 49, 98 and 195 microg FW/ml, respectively. A U-shaped dose-response curve was derived for M. globosum indicating genotoxic effects in high doses and antigenotoxic effects in low doses. M. globosum extract had total phenolics (15 mg/g FW) with flavonoids (aglycones and conjugates: 8 mg/g FW) and proanthocyanidins (3mg/g FW) as major phenolic subclasses. The hydrolysis of conjugated flavonoids yielded the aglycones quercetin (606 microg/g FW) and kaempferol (117.8 microg/g FW) while HPLC-MS/MS analysis of the total extract revealed free flavonoids such as quercetin (19.2 microg/g FW) and myricetin (2.5 microg/g FW). The antioxidant activity of the extract of M. globosum, assessed by the FRAP and TEAC assays yielded values of 275+/-3.82 micromol/g FW and 346+/-4.2 micromol/g FW, respectively. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/16934955/Assessment_of_the_DNA_damaging_potency_and_chemopreventive_effects_towards_BaP_induced_genotoxicity_in_human_derived_cells_by_Monimiastrum_globosum_an_endemic_Mauritian_plant_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(06)00132-9 DB - PRIME DP - Unbound Medicine ER -