TY - JOUR T1 - Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma. AU - Yau,Wai-Ping, AU - Vathsala,Anantharaman, AU - Lou,Huei-Xin, AU - Zhou,Shu Feng, AU - Chan,Eli, Y1 - 2006/08/28/ PY - 2006/06/03/received PY - 2006/07/18/revised PY - 2006/07/27/accepted PY - 2006/8/29/pubmed PY - 2007/4/3/medline PY - 2006/8/29/entrez SP - 313 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 846 IS - 1-2 N2 - A reversed-phase HPLC-UV method, involving simple instrumental setup and mobile phase without ion-pairing reagent, was developed and validated for direct simultaneous quantification of free mycophenolic acid (MPA) and its major metabolite MPA-glucuronide (MPAG) in human plasma. Both free MPA and MPAG were isolated from plasma samples using ultrafiltration prior to analysis. Each chromatographic run was completed within 13 min. The optimized method showed good performance in terms of specificity, linearity (r(2)=0.9999), sensitivity (limit of quantitation (LOQ): 0.005 mg/L for MPA; 1 mg/L for MPAG), and intra- and inter-day precision (R.S.D.<7%). This assay was successfully applied to free MPA and MPAG measurements in clinical samples. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16935575/Simple_reversed_phase_liquid_chromatographic_assay_for_simultaneous_quantification_of_free_mycophenolic_acid_and_its_glucuronide_metabolite_in_human_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00636-2 DB - PRIME DP - Unbound Medicine ER -