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Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids.
Nutr Metab Cardiovasc Dis 2006; 16(6):405-17NM

Abstract

AIM

We examined the effect of meal fatty acids on lipid and apolipoprotein concentrations of very low density lipoprotein (VLDL) and chylomicron/chylomicron remnants in lipid fractions with a Svedberg flotation rate (Sf) 60-400 and Sf 20-60.

METHODS AND RESULTS

Six healthy middle-aged men received in random order mixed meals enriched with saturated (SFA), polyunsaturated (PUFA) or monounsaturated (MUFA) fatty acids on 3 occasions. VLDL and chylomicron/chylomicron remnants in the lipid fractions were separated by immunoaffinity chromatography against apo B-100. In the Sf 60-400 chylomicron/chylomicron remnants, triacylglycerol and cholesterol concentrations were significantly lower following PUFA compared with SFA and MUFA (P < or = 0.05). Apolipoprotein (apo) E responses were significantly higher after SFA in chylomicron/chylomicron remnants and VLDL compared with PUFA and MUFA (P < 0.007). However, apo B responses (particle number) were higher following MUFA than SFA (P = 0.039 for chylomicron/chylomicron remnants). Composition of the chylomicron/chylomicron remnants (expressed per particle) revealed differences in their triacylglycerol and apo E contents; in the Sf 60-400 fraction, SFA-rich chylomicron/chylomicron remnants contained significantly more triacylglycerol than MUFA (P = 0.028), more apo E than PUFA- and MUFA-rich particles (P < 0.05) and in the Sf 20-60 fraction, more apo E than MUFA (P = 0.009).

CONCLUSION

There are specific differences in the composition of chylomicron/chylomicron remnants formed after saturated compared with unsaturated fatty acid-rich meals which could determine their metabolic fate in the circulation and subsequent atherogenicity.

Authors+Show Affiliations

Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, The University of Reading, Reading, Berkshire RG6 6AP, UK. k.g.jackson@reading.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16935699

Citation

Jackson, Kim G., et al. "Apolipoprotein E Enrichment of Immuno-separated Chylomicron and Chylomicron Remnants Following Saturated Fatty Acids." Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, vol. 16, no. 6, 2006, pp. 405-17.
Jackson KG, Wolstencroft EJ, Bateman PA, et al. Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids. Nutr Metab Cardiovasc Dis. 2006;16(6):405-17.
Jackson, K. G., Wolstencroft, E. J., Bateman, P. A., Yaqoob, P., & Williams, C. M. (2006). Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids. Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, 16(6), pp. 405-17.
Jackson KG, et al. Apolipoprotein E Enrichment of Immuno-separated Chylomicron and Chylomicron Remnants Following Saturated Fatty Acids. Nutr Metab Cardiovasc Dis. 2006;16(6):405-17. PubMed PMID: 16935699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids. AU - Jackson,Kim G, AU - Wolstencroft,Emma J, AU - Bateman,Paul A, AU - Yaqoob,Parveen, AU - Williams,Christine M, Y1 - 2005/11/02/ PY - 2005/03/23/received PY - 2005/07/11/revised PY - 2005/07/11/accepted PY - 2006/8/29/pubmed PY - 2006/9/23/medline PY - 2006/8/29/entrez SP - 405 EP - 17 JF - Nutrition, metabolism, and cardiovascular diseases : NMCD JO - Nutr Metab Cardiovasc Dis VL - 16 IS - 6 N2 - AIM: We examined the effect of meal fatty acids on lipid and apolipoprotein concentrations of very low density lipoprotein (VLDL) and chylomicron/chylomicron remnants in lipid fractions with a Svedberg flotation rate (Sf) 60-400 and Sf 20-60. METHODS AND RESULTS: Six healthy middle-aged men received in random order mixed meals enriched with saturated (SFA), polyunsaturated (PUFA) or monounsaturated (MUFA) fatty acids on 3 occasions. VLDL and chylomicron/chylomicron remnants in the lipid fractions were separated by immunoaffinity chromatography against apo B-100. In the Sf 60-400 chylomicron/chylomicron remnants, triacylglycerol and cholesterol concentrations were significantly lower following PUFA compared with SFA and MUFA (P < or = 0.05). Apolipoprotein (apo) E responses were significantly higher after SFA in chylomicron/chylomicron remnants and VLDL compared with PUFA and MUFA (P < 0.007). However, apo B responses (particle number) were higher following MUFA than SFA (P = 0.039 for chylomicron/chylomicron remnants). Composition of the chylomicron/chylomicron remnants (expressed per particle) revealed differences in their triacylglycerol and apo E contents; in the Sf 60-400 fraction, SFA-rich chylomicron/chylomicron remnants contained significantly more triacylglycerol than MUFA (P = 0.028), more apo E than PUFA- and MUFA-rich particles (P < 0.05) and in the Sf 20-60 fraction, more apo E than MUFA (P = 0.009). CONCLUSION: There are specific differences in the composition of chylomicron/chylomicron remnants formed after saturated compared with unsaturated fatty acid-rich meals which could determine their metabolic fate in the circulation and subsequent atherogenicity. SN - 1590-3729 UR - https://www.unboundmedicine.com/medline/citation/16935699/Apolipoprotein_E_enrichment_of_immuno_separated_chylomicron_and_chylomicron_remnants_following_saturated_fatty_acids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-4753(05)00147-X DB - PRIME DP - Unbound Medicine ER -