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Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids.

Abstract

AIM

We examined the effect of meal fatty acids on lipid and apolipoprotein concentrations of very low density lipoprotein (VLDL) and chylomicron/chylomicron remnants in lipid fractions with a Svedberg flotation rate (Sf) 60-400 and Sf 20-60.

METHODS AND RESULTS

Six healthy middle-aged men received in random order mixed meals enriched with saturated (SFA), polyunsaturated (PUFA) or monounsaturated (MUFA) fatty acids on 3 occasions. VLDL and chylomicron/chylomicron remnants in the lipid fractions were separated by immunoaffinity chromatography against apo B-100. In the Sf 60-400 chylomicron/chylomicron remnants, triacylglycerol and cholesterol concentrations were significantly lower following PUFA compared with SFA and MUFA (P < or = 0.05). Apolipoprotein (apo) E responses were significantly higher after SFA in chylomicron/chylomicron remnants and VLDL compared with PUFA and MUFA (P < 0.007). However, apo B responses (particle number) were higher following MUFA than SFA (P = 0.039 for chylomicron/chylomicron remnants). Composition of the chylomicron/chylomicron remnants (expressed per particle) revealed differences in their triacylglycerol and apo E contents; in the Sf 60-400 fraction, SFA-rich chylomicron/chylomicron remnants contained significantly more triacylglycerol than MUFA (P = 0.028), more apo E than PUFA- and MUFA-rich particles (P < 0.05) and in the Sf 20-60 fraction, more apo E than MUFA (P = 0.009).

CONCLUSION

There are specific differences in the composition of chylomicron/chylomicron remnants formed after saturated compared with unsaturated fatty acid-rich meals which could determine their metabolic fate in the circulation and subsequent atherogenicity.

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  • Authors+Show Affiliations

    ,

    Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, The University of Reading, Reading, Berkshire RG6 6AP, UK. k.g.jackson@reading.ac.uk

    , , ,

    Source

    MeSH

    Adult
    Apolipoprotein C-III
    Apolipoproteins C
    Apolipoproteins E
    Chylomicron Remnants
    Chylomicrons
    Dietary Fats, Unsaturated
    Fasting
    Fatty Acids
    Fatty Acids, Monounsaturated
    Fatty Acids, Unsaturated
    Humans
    Lipoproteins, VLDL
    Male
    Middle Aged
    Triglycerides

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16935699

    Citation

    Jackson, Kim G., et al. "Apolipoprotein E Enrichment of Immuno-separated Chylomicron and Chylomicron Remnants Following Saturated Fatty Acids." Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, vol. 16, no. 6, 2006, pp. 405-17.
    Jackson KG, Wolstencroft EJ, Bateman PA, et al. Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids. Nutr Metab Cardiovasc Dis. 2006;16(6):405-17.
    Jackson, K. G., Wolstencroft, E. J., Bateman, P. A., Yaqoob, P., & Williams, C. M. (2006). Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids. Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, 16(6), pp. 405-17.
    Jackson KG, et al. Apolipoprotein E Enrichment of Immuno-separated Chylomicron and Chylomicron Remnants Following Saturated Fatty Acids. Nutr Metab Cardiovasc Dis. 2006;16(6):405-17. PubMed PMID: 16935699.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Apolipoprotein E enrichment of immuno-separated chylomicron and chylomicron remnants following saturated fatty acids. AU - Jackson,Kim G, AU - Wolstencroft,Emma J, AU - Bateman,Paul A, AU - Yaqoob,Parveen, AU - Williams,Christine M, Y1 - 2005/11/02/ PY - 2005/03/23/received PY - 2005/07/11/revised PY - 2005/07/11/accepted PY - 2006/8/29/pubmed PY - 2006/9/23/medline PY - 2006/8/29/entrez SP - 405 EP - 17 JF - Nutrition, metabolism, and cardiovascular diseases : NMCD JO - Nutr Metab Cardiovasc Dis VL - 16 IS - 6 N2 - AIM: We examined the effect of meal fatty acids on lipid and apolipoprotein concentrations of very low density lipoprotein (VLDL) and chylomicron/chylomicron remnants in lipid fractions with a Svedberg flotation rate (Sf) 60-400 and Sf 20-60. METHODS AND RESULTS: Six healthy middle-aged men received in random order mixed meals enriched with saturated (SFA), polyunsaturated (PUFA) or monounsaturated (MUFA) fatty acids on 3 occasions. VLDL and chylomicron/chylomicron remnants in the lipid fractions were separated by immunoaffinity chromatography against apo B-100. In the Sf 60-400 chylomicron/chylomicron remnants, triacylglycerol and cholesterol concentrations were significantly lower following PUFA compared with SFA and MUFA (P < or = 0.05). Apolipoprotein (apo) E responses were significantly higher after SFA in chylomicron/chylomicron remnants and VLDL compared with PUFA and MUFA (P < 0.007). However, apo B responses (particle number) were higher following MUFA than SFA (P = 0.039 for chylomicron/chylomicron remnants). Composition of the chylomicron/chylomicron remnants (expressed per particle) revealed differences in their triacylglycerol and apo E contents; in the Sf 60-400 fraction, SFA-rich chylomicron/chylomicron remnants contained significantly more triacylglycerol than MUFA (P = 0.028), more apo E than PUFA- and MUFA-rich particles (P < 0.05) and in the Sf 20-60 fraction, more apo E than MUFA (P = 0.009). CONCLUSION: There are specific differences in the composition of chylomicron/chylomicron remnants formed after saturated compared with unsaturated fatty acid-rich meals which could determine their metabolic fate in the circulation and subsequent atherogenicity. SN - 1590-3729 UR - https://www.unboundmedicine.com/medline/citation/16935699/Apolipoprotein_E_enrichment_of_immuno_separated_chylomicron_and_chylomicron_remnants_following_saturated_fatty_acids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-4753(05)00147-X DB - PRIME DP - Unbound Medicine ER -