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Clinical and immunological characteristics of patients with Sjögren's syndrome in relation to alpha-fodrin antibodies.
Rheumatology (Oxford). 2007 Mar; 46(3):479-83.R

Abstract

OBJECTIVES

To analyse the prevalence of alpha-fodrin antibodies in patients with primary (pSS) and secondary Sjögren's syndrome (sSS) and the relation to clinical, serological and immunological features.

METHODS

Serum IgA and IgG antibodies to the 120 kDa alpha-fodrin were determined by ELISA technique in 62 pSS patients and 28 sSS patients. Results were correlated with clinical symptoms and laboratory findings as well as with the HLA-DR genotype. Additionally, antibody concentrations were correlated with the numbers of peripheral blood mononuclear cells (PBMCs) secreting interleukin (IL)-6, IL-10, interferon-gamma (INF)-gamma, and tumour necrosis factor-alpha determined by ELISPOT analysis. Lymphocytes and monocytes were examined flow-cytometrically for the expression of activation markers. Healthy age- and sex-matched volunteers served as controls.

RESULTS

The sensitivity of IgA and IgG alpha-fodrin antibodies was 35 and 31%, respectively, in pSS patients. In sSS patients, the sensitivity was 29 and 21%, respectively. In pSS patients with IgG antibodies, recurrent parotid swelling was significantly more prevalent. Also the number of INF-gamma secreting PBMCs and the percentage of CD4/CD71+ lymphocytes as well as CD14/HLA-DR+ monocytes were significantly increased in this group compared with alpha-fodrin-negative patients or with controls. Interestingly, these patients also had a shorter disease duration. No association of alpha-fodrin antibodies with the HLA-DR genotype was found.

CONCLUSION

Due to the low prevalence, serum antibodies to alpha-fodrin turned out to be of limited diagnostic value in our study. However, our data suggest that IgG antibodies to alpha-fodrin are indicative of clinical and immunological activity in pSS especially in patients with shorter disease duration and may thus serve as a marker of disease activity.

Authors+Show Affiliations

Department of Medicine B, Muenster University Hospital, Muenster, Germany. willeke@mednet.uni-muenster.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16935916

Citation

Willeke, P, et al. "Clinical and Immunological Characteristics of Patients With Sjögren's Syndrome in Relation to Alpha-fodrin Antibodies." Rheumatology (Oxford, England), vol. 46, no. 3, 2007, pp. 479-83.
Willeke P, Gaubitz M, Schotte H, et al. Clinical and immunological characteristics of patients with Sjögren's syndrome in relation to alpha-fodrin antibodies. Rheumatology (Oxford). 2007;46(3):479-83.
Willeke, P., Gaubitz, M., Schotte, H., Becker, H., Mickholz, E., Domschke, W., & Schlüter, B. (2007). Clinical and immunological characteristics of patients with Sjögren's syndrome in relation to alpha-fodrin antibodies. Rheumatology (Oxford, England), 46(3), 479-83.
Willeke P, et al. Clinical and Immunological Characteristics of Patients With Sjögren's Syndrome in Relation to Alpha-fodrin Antibodies. Rheumatology (Oxford). 2007;46(3):479-83. PubMed PMID: 16935916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical and immunological characteristics of patients with Sjögren's syndrome in relation to alpha-fodrin antibodies. AU - Willeke,P, AU - Gaubitz,M, AU - Schotte,H, AU - Becker,H, AU - Mickholz,E, AU - Domschke,W, AU - Schlüter,B, Y1 - 2006/08/25/ PY - 2006/8/29/pubmed PY - 2007/5/10/medline PY - 2006/8/29/entrez SP - 479 EP - 83 JF - Rheumatology (Oxford, England) JO - Rheumatology (Oxford) VL - 46 IS - 3 N2 - OBJECTIVES: To analyse the prevalence of alpha-fodrin antibodies in patients with primary (pSS) and secondary Sjögren's syndrome (sSS) and the relation to clinical, serological and immunological features. METHODS: Serum IgA and IgG antibodies to the 120 kDa alpha-fodrin were determined by ELISA technique in 62 pSS patients and 28 sSS patients. Results were correlated with clinical symptoms and laboratory findings as well as with the HLA-DR genotype. Additionally, antibody concentrations were correlated with the numbers of peripheral blood mononuclear cells (PBMCs) secreting interleukin (IL)-6, IL-10, interferon-gamma (INF)-gamma, and tumour necrosis factor-alpha determined by ELISPOT analysis. Lymphocytes and monocytes were examined flow-cytometrically for the expression of activation markers. Healthy age- and sex-matched volunteers served as controls. RESULTS: The sensitivity of IgA and IgG alpha-fodrin antibodies was 35 and 31%, respectively, in pSS patients. In sSS patients, the sensitivity was 29 and 21%, respectively. In pSS patients with IgG antibodies, recurrent parotid swelling was significantly more prevalent. Also the number of INF-gamma secreting PBMCs and the percentage of CD4/CD71+ lymphocytes as well as CD14/HLA-DR+ monocytes were significantly increased in this group compared with alpha-fodrin-negative patients or with controls. Interestingly, these patients also had a shorter disease duration. No association of alpha-fodrin antibodies with the HLA-DR genotype was found. CONCLUSION: Due to the low prevalence, serum antibodies to alpha-fodrin turned out to be of limited diagnostic value in our study. However, our data suggest that IgG antibodies to alpha-fodrin are indicative of clinical and immunological activity in pSS especially in patients with shorter disease duration and may thus serve as a marker of disease activity. SN - 1462-0324 UR - https://www.unboundmedicine.com/medline/citation/16935916/Clinical_and_immunological_characteristics_of_patients_with_Sjögren's_syndrome_in_relation_to_alpha_fodrin_antibodies_ L2 - https://academic.oup.com/rheumatology/article-lookup/doi/10.1093/rheumatology/kel270 DB - PRIME DP - Unbound Medicine ER -