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Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model.
Biometals. 2006 Oct; 19(5):473-81.B

Abstract

The influence of long-term exposure to cadmium (Cd) on essential minerals was investigated using a Caco-2 TC7 cells and a multi-analytical tool: microwave digestion and inductively coupled plasma mass spectrometry. Intracellular levels, effects on cadmium accumulation, distribution, and reference concentration ranges of the following elements were determined: Na, Mg, Ca, Cr, Fe, Mn, Co, Ni, Cu, Zn, Mo, and Cd. Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 micromol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. Furthermore, this accumulation was more marked in cells exposed long-term to cadmium compared with controls, and that this exposure resulted in a significant accumulation of copper and zinc but not of the other elements measured. Interactions of Cd with three elements: zinc, copper, and manganese were particularly studied. Exposed to 30 micromol/l of the element, manganese showed the highest inhibition and copper the lowest on cadmium intracellular accumulation but Zn, Cu, and Mn behave differently in terms of their mutual competition with Cd. Indeed, increasing cadmium in the culture medium resulted in a gradual and significant increase in the accumulation of zinc. There was a significant decrease in manganese from 5 micromol Cd/l exposure, and no variation was observed with copper.

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Authors+Show Affiliations

Noël L
Agence Française de Sécurité Sanitaire des Aliments, Unité Contaminants Inorganiques et Minéraux de l'Environnement, Equipe Métaux Lourds et Eléments minéraux, AFSSA LERQAP: 23, F-94706, Maisons-Alfort cedex, France. l.noel@afssa.fr
Huynh-Delerme C
No affiliation info available
Guérin T
No affiliation info available
Huet H
No affiliation info available
Frémy JM
No affiliation info available
Kolf-Clauw M
No affiliation info available

MeSH

Caco-2 CellsCadmiumCopperHumansManganeseMass SpectrometryMicrowavesZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16937253

Citation

Noël, Laurent, et al. "Cadmium Accumulation and Interactions With Zinc, Copper, and Manganese, Analysed By ICP-MS in a Long-term Caco-2 TC7 Cell Model." Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, vol. 19, no. 5, 2006, pp. 473-81.
Noël L, Huynh-Delerme C, Guérin T, et al. Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model. Biometals. 2006;19(5):473-81.
Noël, L., Huynh-Delerme, C., Guérin, T., Huet, H., Frémy, J. M., & Kolf-Clauw, M. (2006). Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model. Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, 19(5), 473-81.
Noël L, et al. Cadmium Accumulation and Interactions With Zinc, Copper, and Manganese, Analysed By ICP-MS in a Long-term Caco-2 TC7 Cell Model. Biometals. 2006;19(5):473-81. PubMed PMID: 16937253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model. AU - Noël,Laurent, AU - Huynh-Delerme,Céline, AU - Guérin,Thierry, AU - Huet,Hélène, AU - Frémy,Jean-Marc, AU - Kolf-Clauw,Martine, PY - 2005/08/03/received PY - 2005/11/14/accepted PY - 2006/8/29/pubmed PY - 2007/4/26/medline PY - 2006/8/29/entrez SP - 473 EP - 81 JF - Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine JO - Biometals VL - 19 IS - 5 N2 - The influence of long-term exposure to cadmium (Cd) on essential minerals was investigated using a Caco-2 TC7 cells and a multi-analytical tool: microwave digestion and inductively coupled plasma mass spectrometry. Intracellular levels, effects on cadmium accumulation, distribution, and reference concentration ranges of the following elements were determined: Na, Mg, Ca, Cr, Fe, Mn, Co, Ni, Cu, Zn, Mo, and Cd. Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 micromol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. Furthermore, this accumulation was more marked in cells exposed long-term to cadmium compared with controls, and that this exposure resulted in a significant accumulation of copper and zinc but not of the other elements measured. Interactions of Cd with three elements: zinc, copper, and manganese were particularly studied. Exposed to 30 micromol/l of the element, manganese showed the highest inhibition and copper the lowest on cadmium intracellular accumulation but Zn, Cu, and Mn behave differently in terms of their mutual competition with Cd. Indeed, increasing cadmium in the culture medium resulted in a gradual and significant increase in the accumulation of zinc. There was a significant decrease in manganese from 5 micromol Cd/l exposure, and no variation was observed with copper. SN - 0966-0844 UR - https://www.unboundmedicine.com/medline/citation/16937253/Cadmium_accumulation_and_interactions_with_zinc_copper_and_manganese_analysed_by_ICP_MS_in_a_long_term_Caco_2_TC7_cell_model_ L2 - https://doi.org/10.1007/s10534-005-5147-y DB - PRIME DP - Unbound Medicine ER -