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Respirometric evaluation and modeling of glucose utilization by Escherichia coli under aerobic and mesophilic cultivation conditions.
Biotechnol Bioeng. 2007 Jan 01; 96(1):94-105.BB

Abstract

The study presents a mechanistic model for the evaluation of glucose utilization by Escherichia coli under aerobic and mesophilic growth conditions. In the first step, the experimental data was derived from batch respirometric experiments conducted at 37 degrees C, using two different initial substrate to microorganism (S(0)/X(0)) ratios of 15.0 and 1.3 mgCOD/mgSS. Acetate generation, glycogen formation and oxygen uptake rate profile were monitored together with glucose uptake and biomass increase throughout the experiments. The oxygen uptake rate (OUR) exhibited a typical profile accounting for growth on glucose, acetate and glycogen. No acetate formation (overflow) was detected at low initial S(0)/X(0) ratio. In the second step, the effect of culture history developed under long-term growth limiting conditions on the kinetics of glucose utilization by the same culture was evaluated in a sequencing batch reactor (SBR). The system was operated at cyclic steady state with a constant mean cell residence time of 5 days. The kinetic response of E.coli culture was followed by similar measurements within a complete cycle. Model calibration for the SBR system showed that E. coli culture regulated its growth metabolism by decreasing the maximum growth rate (lower microH) together with an increase of substrate affinity (lower K(S)) as compared to uncontrolled growth conditions. The continuous low rate operation of SBR system induced a significant biochemical substrate storage capability as glycogen in parallel to growth, which persisted throughout the operation. The acetate overflow was observed again as an important mechanism to be accounted for in the evaluation of process kinetics.

Authors+Show Affiliations

Insel G
Department of Environmental Engineering, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
Celikyilmaz G
No affiliation info available
Ucisik-Akkaya E
No affiliation info available
Yesiladali K
No affiliation info available
Cakar ZP
No affiliation info available
Tamerler C
No affiliation info available
Orhon D
No affiliation info available

MeSH

AcetatesAerobiosisBioreactorsCell Culture TechniquesCell ProliferationComputer SimulationEscherichia coliGlucoseHot TemperatureKineticsMetabolic Clearance RateModels, BiologicalOxygenOxygen ConsumptionTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16937401

Citation

Insel, G, et al. "Respirometric Evaluation and Modeling of Glucose Utilization By Escherichia Coli Under Aerobic and Mesophilic Cultivation Conditions." Biotechnology and Bioengineering, vol. 96, no. 1, 2007, pp. 94-105.
Insel G, Celikyilmaz G, Ucisik-Akkaya E, et al. Respirometric evaluation and modeling of glucose utilization by Escherichia coli under aerobic and mesophilic cultivation conditions. Biotechnol Bioeng. 2007;96(1):94-105.
Insel, G., Celikyilmaz, G., Ucisik-Akkaya, E., Yesiladali, K., Cakar, Z. P., Tamerler, C., & Orhon, D. (2007). Respirometric evaluation and modeling of glucose utilization by Escherichia coli under aerobic and mesophilic cultivation conditions. Biotechnology and Bioengineering, 96(1), 94-105.
Insel G, et al. Respirometric Evaluation and Modeling of Glucose Utilization By Escherichia Coli Under Aerobic and Mesophilic Cultivation Conditions. Biotechnol Bioeng. 2007 Jan 1;96(1):94-105. PubMed PMID: 16937401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Respirometric evaluation and modeling of glucose utilization by Escherichia coli under aerobic and mesophilic cultivation conditions. AU - Insel,G, AU - Celikyilmaz,G, AU - Ucisik-Akkaya,E, AU - Yesiladali,K, AU - Cakar,Z P, AU - Tamerler,C, AU - Orhon,D, PY - 2006/8/29/pubmed PY - 2007/3/1/medline PY - 2006/8/29/entrez SP - 94 EP - 105 JF - Biotechnology and bioengineering JO - Biotechnol Bioeng VL - 96 IS - 1 N2 - The study presents a mechanistic model for the evaluation of glucose utilization by Escherichia coli under aerobic and mesophilic growth conditions. In the first step, the experimental data was derived from batch respirometric experiments conducted at 37 degrees C, using two different initial substrate to microorganism (S(0)/X(0)) ratios of 15.0 and 1.3 mgCOD/mgSS. Acetate generation, glycogen formation and oxygen uptake rate profile were monitored together with glucose uptake and biomass increase throughout the experiments. The oxygen uptake rate (OUR) exhibited a typical profile accounting for growth on glucose, acetate and glycogen. No acetate formation (overflow) was detected at low initial S(0)/X(0) ratio. In the second step, the effect of culture history developed under long-term growth limiting conditions on the kinetics of glucose utilization by the same culture was evaluated in a sequencing batch reactor (SBR). The system was operated at cyclic steady state with a constant mean cell residence time of 5 days. The kinetic response of E.coli culture was followed by similar measurements within a complete cycle. Model calibration for the SBR system showed that E. coli culture regulated its growth metabolism by decreasing the maximum growth rate (lower microH) together with an increase of substrate affinity (lower K(S)) as compared to uncontrolled growth conditions. The continuous low rate operation of SBR system induced a significant biochemical substrate storage capability as glycogen in parallel to growth, which persisted throughout the operation. The acetate overflow was observed again as an important mechanism to be accounted for in the evaluation of process kinetics. SN - 0006-3592 UR - https://www.unboundmedicine.com/medline/citation/16937401/Respirometric_evaluation_and_modeling_of_glucose_utilization_by_Escherichia_coli_under_aerobic_and_mesophilic_cultivation_conditions_ L2 - https://doi.org/10.1002/bit.21163 DB - PRIME DP - Unbound Medicine ER -