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RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague.
Proc Natl Acad Sci U S A. 2006 Sep 05; 103(36):13514-9.PN

Abstract

The pathogenic species of Yersinia contain the transcriptional regulator RovA. In Yersinia pseudotuberculosis and Yersinia enterocolitica, RovA regulates expression of the invasion factor invasin (inv), which mediates translocation across the intestinal epithelium. A Y. enterocolitica rovA mutant has a significant decrease in virulence by LD(50) analysis and an altered rate of dissemination compared with either wild type or an inv mutant, suggesting that RovA regulates multiple virulence factors. Here, we show the involvement of RovA in the virulence of Yersinia pestis, which naturally lacks a functional inv gene. A Y. pestis DeltarovA mutant is attenuated approximately 80-fold by LD(50) and is defective in dissemination/colonization of spleens and lungs after s.c. inoculation. However, the DeltarovA mutant is only slightly attenuated when given via an intranasal or i.p. route, indicating a more important role for RovA in bubonic plague than pneumonic plague or systemic infection. Microarray analysis was used to define the RovA regulon. The psa locus was among the most highly down-regulated loci in the DeltarovA mutant. A DeltapsaA mutant had a significant dissemination defect after s.c. infection but only slight attenuation by the pneumonic-disease model, closely mimicking the virulence defect seen with the DeltarovA mutant. DNA-binding studies revealed that RovA specifically interacts with the psaE and psaA promoter regions, indicating a direct role for RovA in regulating this locus. Thus, RovA appears to be a global transcription factor in Y. pestis and, through its regulatory influence on genes such as psaEFABC, contributes to the virulence of Y. pestis.

Authors+Show Affiliations

Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16938880

Citation

Cathelyn, Jason S., et al. "RovA, a Global Regulator of Yersinia Pestis, Specifically Required for Bubonic Plague." Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 36, 2006, pp. 13514-9.
Cathelyn JS, Crosby SD, Lathem WW, et al. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 2006;103(36):13514-9.
Cathelyn, J. S., Crosby, S. D., Lathem, W. W., Goldman, W. E., & Miller, V. L. (2006). RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proceedings of the National Academy of Sciences of the United States of America, 103(36), 13514-9.
Cathelyn JS, et al. RovA, a Global Regulator of Yersinia Pestis, Specifically Required for Bubonic Plague. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. PubMed PMID: 16938880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. AU - Cathelyn,Jason S, AU - Crosby,Seth D, AU - Lathem,Wyndham W, AU - Goldman,William E, AU - Miller,Virginia L, Y1 - 2006/08/28/ PY - 2006/8/30/pubmed PY - 2006/10/20/medline PY - 2006/8/30/entrez SP - 13514 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 103 IS - 36 N2 - The pathogenic species of Yersinia contain the transcriptional regulator RovA. In Yersinia pseudotuberculosis and Yersinia enterocolitica, RovA regulates expression of the invasion factor invasin (inv), which mediates translocation across the intestinal epithelium. A Y. enterocolitica rovA mutant has a significant decrease in virulence by LD(50) analysis and an altered rate of dissemination compared with either wild type or an inv mutant, suggesting that RovA regulates multiple virulence factors. Here, we show the involvement of RovA in the virulence of Yersinia pestis, which naturally lacks a functional inv gene. A Y. pestis DeltarovA mutant is attenuated approximately 80-fold by LD(50) and is defective in dissemination/colonization of spleens and lungs after s.c. inoculation. However, the DeltarovA mutant is only slightly attenuated when given via an intranasal or i.p. route, indicating a more important role for RovA in bubonic plague than pneumonic plague or systemic infection. Microarray analysis was used to define the RovA regulon. The psa locus was among the most highly down-regulated loci in the DeltarovA mutant. A DeltapsaA mutant had a significant dissemination defect after s.c. infection but only slight attenuation by the pneumonic-disease model, closely mimicking the virulence defect seen with the DeltarovA mutant. DNA-binding studies revealed that RovA specifically interacts with the psaE and psaA promoter regions, indicating a direct role for RovA in regulating this locus. Thus, RovA appears to be a global transcription factor in Y. pestis and, through its regulatory influence on genes such as psaEFABC, contributes to the virulence of Y. pestis. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/16938880/RovA_a_global_regulator_of_Yersinia_pestis_specifically_required_for_bubonic_plague_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=16938880 DB - PRIME DP - Unbound Medicine ER -