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[Effect of prostaglandin E1 on monocyte chemotactic protein-1 expression in Kupffer cells of rats with hepatic ischemia-reperfusion injury].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Aug; 26(8):1180-3.NF

Abstract

OBJECTIVE

To observe the effect of prostaglandin E(1) (PGE(1)) on the expression of monocyte chemotactic protein-1 (MCP-1) in Kupffer cells (KCs) of rats with hepatic ischemia-reperfusion injury (IRI).

METHODS

Seventy-two SD rats were randomized into sham operation group, ischemia-reperfusion group (I/R group) and PGE(1) treatment group (PGE(1) group). Rat models of partial warm ischemia-reperfusion injury of the liver was established, and in PGE(1) group, PGE(1) were given 10 min before the operation. At 1, 6, 12 and 24 h after the reperfusion, blood sample was taken from the inferior vena cava for measuring alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. The KCs were isolated and incubated in vitro, and tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in the supernatant were measured by enzyme-linked immunosorbent assay. MCP-1 expression in the KCs was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS

ALT and AST levels of the PGE(1) group were significantly lower than I/R group (P<0.01). The mRNA and protein expression of MCP-1 and the TNF-alpha and IL-1beta levels in the I/R group significantly increased in the course of reperfusion and slightly decreased at 24 h, but were still significantly higher than those in the sham operation group (P<0.05). The expression of these factors were markedly decreased after PGE(1) treatment as compared with the I/R group (P<0.05).

CONCLUSION

PGE(1) can protect against ischemia-reperfusion injury of the rat liver partially by suppressing KCs activation, reducing excessive release of TNF-alpha and IL-1beta from KCs and decreasing the high expression of MCP-1 protein and mRNA.

Authors+Show Affiliations

Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. mario_doctor@163.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16939916

Citation

Ma, Wei, et al. "[Effect of Prostaglandin E1 On Monocyte Chemotactic Protein-1 Expression in Kupffer Cells of Rats With Hepatic Ischemia-reperfusion Injury]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 8, 2006, pp. 1180-3.
Ma W, Wang ZR, Zhang YF, et al. [Effect of prostaglandin E1 on monocyte chemotactic protein-1 expression in Kupffer cells of rats with hepatic ischemia-reperfusion injury]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(8):1180-3.
Ma, W., Wang, Z. R., Zhang, Y. F., & Shi, L. (2006). [Effect of prostaglandin E1 on monocyte chemotactic protein-1 expression in Kupffer cells of rats with hepatic ischemia-reperfusion injury]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(8), 1180-3.
Ma W, et al. [Effect of Prostaglandin E1 On Monocyte Chemotactic Protein-1 Expression in Kupffer Cells of Rats With Hepatic Ischemia-reperfusion Injury]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(8):1180-3. PubMed PMID: 16939916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of prostaglandin E1 on monocyte chemotactic protein-1 expression in Kupffer cells of rats with hepatic ischemia-reperfusion injury]. AU - Ma,Wei, AU - Wang,Zuo-ren, AU - Zhang,Yun-feng, AU - Shi,Lei, PY - 2006/8/31/pubmed PY - 2008/5/6/medline PY - 2006/8/31/entrez SP - 1180 EP - 3 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 8 N2 - OBJECTIVE: To observe the effect of prostaglandin E(1) (PGE(1)) on the expression of monocyte chemotactic protein-1 (MCP-1) in Kupffer cells (KCs) of rats with hepatic ischemia-reperfusion injury (IRI). METHODS: Seventy-two SD rats were randomized into sham operation group, ischemia-reperfusion group (I/R group) and PGE(1) treatment group (PGE(1) group). Rat models of partial warm ischemia-reperfusion injury of the liver was established, and in PGE(1) group, PGE(1) were given 10 min before the operation. At 1, 6, 12 and 24 h after the reperfusion, blood sample was taken from the inferior vena cava for measuring alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. The KCs were isolated and incubated in vitro, and tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in the supernatant were measured by enzyme-linked immunosorbent assay. MCP-1 expression in the KCs was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: ALT and AST levels of the PGE(1) group were significantly lower than I/R group (P<0.01). The mRNA and protein expression of MCP-1 and the TNF-alpha and IL-1beta levels in the I/R group significantly increased in the course of reperfusion and slightly decreased at 24 h, but were still significantly higher than those in the sham operation group (P<0.05). The expression of these factors were markedly decreased after PGE(1) treatment as compared with the I/R group (P<0.05). CONCLUSION: PGE(1) can protect against ischemia-reperfusion injury of the rat liver partially by suppressing KCs activation, reducing excessive release of TNF-alpha and IL-1beta from KCs and decreasing the high expression of MCP-1 protein and mRNA. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/16939916/[Effect_of_prostaglandin_E1_on_monocyte_chemotactic_protein_1_expression_in_Kupffer_cells_of_rats_with_hepatic_ischemia_reperfusion_injury]_ L2 - https://antibodies.cancer.gov/detail/CPTC-OTUB1-1 DB - PRIME DP - Unbound Medicine ER -