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Interleukin-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide in Iranian patients with aggressive periodontitis.
Med Sci Monit. 2006 Sep; 12(9):CR393-6.MS

Abstract

BACKGROUND

It has been suggested that hyper-responsiveness of monocytes to the products of dental plaque, especially the endotoxin of Gram-negative bacteria and the secretion of high levels of pro-inflammatory cytokines, may have a role in the pathogenesis of aggressive periodontitis (AP). To investigate this possibility, IL-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide (LPS) in AP patients was evaluated.

MATERIAL/METHODS

Fifteen patients with AP were compared with 15 periodontally healthy controls in a case control study. Monocytes were obtained from peripheral blood samples and cultured. The reaction of monocytes was studied by their IL-6 production using ELISA before and six hours after stimulation by 0.1 microg/ml E. col. LPS. The Wilcoxon and Mann-Whitney U tests were used to compare the groups.

RESULTS

There was no significant difference in IL-6 production levels before LPS stimulation between patients and controls. Upon LPS stimulation, IL-6 levels increased in both the patient and control groups compared with their IL-6 levels before stimulation. IL-6 production after LPS stimulation in the patients was higher than controls, but this was not statistically significant. However, the increase in IL-6 production as a result of LPS stimulation was significantly higher in patients than in controls.

CONCLUSIONS

Increased monocyte responsiveness may play a potential role in the pathogenesis of AP. However, whether this is an intrinsic characteristic of the monocyte/macrophage cells of AP patients or just a priming effect as a result of the periodontal disease remains to be investigated.

Authors+Show Affiliations

Periodontology Department, School of Dentistry and Mashhad Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16940934

Citation

Bajestan, Mahboobeh N., et al. "Interleukin-6 Production By Cultured Peripheral Blood Monocytes Before and After Stimulation By E. Coli Lipopolysaccharide in Iranian Patients With Aggressive Periodontitis." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 12, no. 9, 2006, pp. CR393-6.
Bajestan MN, Radvar M, Afshari JT, et al. Interleukin-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide in Iranian patients with aggressive periodontitis. Med Sci Monit. 2006;12(9):CR393-6.
Bajestan, M. N., Radvar, M., Afshari, J. T., Naseh, M. R., & Arab, H. R. (2006). Interleukin-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide in Iranian patients with aggressive periodontitis. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 12(9), CR393-6.
Bajestan MN, et al. Interleukin-6 Production By Cultured Peripheral Blood Monocytes Before and After Stimulation By E. Coli Lipopolysaccharide in Iranian Patients With Aggressive Periodontitis. Med Sci Monit. 2006;12(9):CR393-6. PubMed PMID: 16940934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interleukin-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide in Iranian patients with aggressive periodontitis. AU - Bajestan,Mahboobeh N, AU - Radvar,Mehrdad, AU - Afshari,Jalil T, AU - Naseh,Mohammad R, AU - Arab,Hamid R, PY - 2005/11/29/received PY - 2006/03/31/accepted PY - 2006/8/31/pubmed PY - 2007/1/11/medline PY - 2006/8/31/entrez SP - CR393 EP - 6 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 12 IS - 9 N2 - BACKGROUND: It has been suggested that hyper-responsiveness of monocytes to the products of dental plaque, especially the endotoxin of Gram-negative bacteria and the secretion of high levels of pro-inflammatory cytokines, may have a role in the pathogenesis of aggressive periodontitis (AP). To investigate this possibility, IL-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide (LPS) in AP patients was evaluated. MATERIAL/METHODS: Fifteen patients with AP were compared with 15 periodontally healthy controls in a case control study. Monocytes were obtained from peripheral blood samples and cultured. The reaction of monocytes was studied by their IL-6 production using ELISA before and six hours after stimulation by 0.1 microg/ml E. col. LPS. The Wilcoxon and Mann-Whitney U tests were used to compare the groups. RESULTS: There was no significant difference in IL-6 production levels before LPS stimulation between patients and controls. Upon LPS stimulation, IL-6 levels increased in both the patient and control groups compared with their IL-6 levels before stimulation. IL-6 production after LPS stimulation in the patients was higher than controls, but this was not statistically significant. However, the increase in IL-6 production as a result of LPS stimulation was significantly higher in patients than in controls. CONCLUSIONS: Increased monocyte responsiveness may play a potential role in the pathogenesis of AP. However, whether this is an intrinsic characteristic of the monocyte/macrophage cells of AP patients or just a priming effect as a result of the periodontal disease remains to be investigated. SN - 1234-1010 UR - https://www.unboundmedicine.com/medline/citation/16940934/Interleukin_6_production_by_cultured_peripheral_blood_monocytes_before_and_after_stimulation_by_E__coli_lipopolysaccharide_in_Iranian_patients_with_aggressive_periodontitis_ L2 - https://www.medscimonit.com/download/index/idArt/455260 DB - PRIME DP - Unbound Medicine ER -