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Ultra-performance liquid chromatography/tandem mass spectrometry method for the determination of lercanidipine in human plasma.
Rapid Commun Mass Spectrom. 2006; 20(19):2939-46.RC

Abstract

A simple, sensitive and rapid ultra-performance liquid chromatography/positive electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma. Lercanidipine and the internal standard, nicardipine, were extracted from plasma by liquid-liquid extraction using tert-butyl methyl ether as the extraction solvent. UPLC analysis was performed isocratically on an AcQuity UPLC BEH C18 analytical column (2.1 x 50.0 mm i.d., particle size 1.7 microm). The mobile phase consisted of 70% acetonitrile in water containing 0.2% v/v formic acid and pumped at a flow rate of 0.30 mL/min. ESI in positive ion mode, with multiple reaction monitoring (MRM), was chosen for the detection of the analytes. The assay was linear over a concentration range of 0.05-30 ng/mL for lercanidipine with a limit of quantitation of 0.05 ng/mL. Quality control samples (0.05, 0.15, 15 and 25 ng/mL) in five replicates from five of analytical runs demonstrated intra-assay precision (% CV < or =7.3%), inter-assay precision (% CV < or =6.1%) and an overall accuracy (% relative error) of less than 6.2%. A run time of less than 1.0 min for each sample made it possible to analyze a large number of human plasma samples per day. The method can be used to quantify lercanidipine in human plasma covering a variety of pharmacokinetic or bioequivalence studies.

Authors+Show Affiliations

Kalovidouris M
University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Panepistimiopolis, Zografou 157 71, Athens, Greece.
Michalea S
No affiliation info available
Robola N
No affiliation info available
Koutsopoulou M
No affiliation info available
Panderi I
No affiliation info available

MeSH

Calcium Channel BlockersChromatography, High Pressure LiquidDihydropyridinesHumansReproducibility of ResultsSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16941725

Citation

Kalovidouris, Madgalene, et al. "Ultra-performance Liquid Chromatography/tandem Mass Spectrometry Method for the Determination of Lercanidipine in Human Plasma." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 19, 2006, pp. 2939-46.
Kalovidouris M, Michalea S, Robola N, et al. Ultra-performance liquid chromatography/tandem mass spectrometry method for the determination of lercanidipine in human plasma. Rapid Commun Mass Spectrom. 2006;20(19):2939-46.
Kalovidouris, M., Michalea, S., Robola, N., Koutsopoulou, M., & Panderi, I. (2006). Ultra-performance liquid chromatography/tandem mass spectrometry method for the determination of lercanidipine in human plasma. Rapid Communications in Mass Spectrometry : RCM, 20(19), 2939-46.
Kalovidouris M, et al. Ultra-performance Liquid Chromatography/tandem Mass Spectrometry Method for the Determination of Lercanidipine in Human Plasma. Rapid Commun Mass Spectrom. 2006;20(19):2939-46. PubMed PMID: 16941725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra-performance liquid chromatography/tandem mass spectrometry method for the determination of lercanidipine in human plasma. AU - Kalovidouris,Madgalene, AU - Michalea,Stavroula, AU - Robola,Nikoleta, AU - Koutsopoulou,Maria, AU - Panderi,Irene, PY - 2006/8/31/pubmed PY - 2007/9/7/medline PY - 2006/8/31/entrez SP - 2939 EP - 46 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 20 IS - 19 N2 - A simple, sensitive and rapid ultra-performance liquid chromatography/positive electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma. Lercanidipine and the internal standard, nicardipine, were extracted from plasma by liquid-liquid extraction using tert-butyl methyl ether as the extraction solvent. UPLC analysis was performed isocratically on an AcQuity UPLC BEH C18 analytical column (2.1 x 50.0 mm i.d., particle size 1.7 microm). The mobile phase consisted of 70% acetonitrile in water containing 0.2% v/v formic acid and pumped at a flow rate of 0.30 mL/min. ESI in positive ion mode, with multiple reaction monitoring (MRM), was chosen for the detection of the analytes. The assay was linear over a concentration range of 0.05-30 ng/mL for lercanidipine with a limit of quantitation of 0.05 ng/mL. Quality control samples (0.05, 0.15, 15 and 25 ng/mL) in five replicates from five of analytical runs demonstrated intra-assay precision (% CV < or =7.3%), inter-assay precision (% CV < or =6.1%) and an overall accuracy (% relative error) of less than 6.2%. A run time of less than 1.0 min for each sample made it possible to analyze a large number of human plasma samples per day. The method can be used to quantify lercanidipine in human plasma covering a variety of pharmacokinetic or bioequivalence studies. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16941725/Ultra_performance_liquid_chromatography/tandem_mass_spectrometry_method_for_the_determination_of_lercanidipine_in_human_plasma_ L2 - https://doi.org/10.1002/rcm.2693 DB - PRIME DP - Unbound Medicine ER -
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