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Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst.
Mol Plant Microbe Interact. 2006 Sep; 19(9):1023-33.MP

Abstract

Genetic similarities between plant interactions with microbial pathogens and wheat interactions with Hessian fly larvae prompted us to investigate defense and counterdefense mechanisms. Plant oxidative burst, a rapid increase in the levels of active oxygen species (AOS) within the initial 24 h of an interaction with pathogens, commonly is associated with defenses that are triggered by gene-for-gene recognition events similar to those involving wheat and Hessian fly larvae. RNAs encoded by Hessian fly superoxide dismutase (SOD) and catalase (CAT) genes, involved in detoxification of AOS, increased in first-instar larvae during both compatible and incompatible interactions. However, mRNA levels of a wheat NADPH oxidase (NOX) gene that generates superoxide (O2-) did not increase. In addition, inhibiting wheat NOX enzyme with diphenyleneiodonium did not result in increased survival of avirulent larvae. However, nitro blue tetrazolium staining indicated that basal levels of O2- are present in both uninfested and infested wheat tissue. mRNA encoded by wheat genes involved in detoxification of the cellular environment, SOD, CAT, and glutathione-S-transferase did not increase in abundance. Histochemical staining with 3,3-diaminobenzidine revealed no increases in wheat hydrogen peroxide (H2O2) during infestation that were correlated with the changes in larval SOD and CAT mRNA. However, treatment with 2',7'-dichlorofluorescin demonstrated the presence of basal levels of H2O2 in the elongation zone of both infested and uninfested plants. The accumulation of a wheat flavanone 3-hydroxylase mRNA did show some parallels with larval gene mRNA profiles. These results suggested that larvae encounter stresses imposed by mechanisms other than an oxidative burst in wheat seedlings.

Authors+Show Affiliations

Department of Agronomy, Purdue University, 915 W. State St., West Lafayette, IN 47907, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16941906

Citation

Giovanini, Marcelo P., et al. "Gene-for-gene Defense of Wheat Against the Hessian Fly Lacks a Classical Oxidative Burst." Molecular Plant-microbe Interactions : MPMI, vol. 19, no. 9, 2006, pp. 1023-33.
Giovanini MP, Puthoff DP, Nemacheck JA, et al. Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst. Mol Plant Microbe Interact. 2006;19(9):1023-33.
Giovanini, M. P., Puthoff, D. P., Nemacheck, J. A., Mittapalli, O., Saltzmann, K. D., Ohm, H. W., Shukle, R. H., & Williams, C. E. (2006). Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst. Molecular Plant-microbe Interactions : MPMI, 19(9), 1023-33.
Giovanini MP, et al. Gene-for-gene Defense of Wheat Against the Hessian Fly Lacks a Classical Oxidative Burst. Mol Plant Microbe Interact. 2006;19(9):1023-33. PubMed PMID: 16941906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst. AU - Giovanini,Marcelo P, AU - Puthoff,David P, AU - Nemacheck,Jill A, AU - Mittapalli,Omprakash, AU - Saltzmann,Kurt D, AU - Ohm,Herbert W, AU - Shukle,Richard H, AU - Williams,Christie E, PY - 2006/9/1/pubmed PY - 2006/10/28/medline PY - 2006/9/1/entrez SP - 1023 EP - 33 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 19 IS - 9 N2 - Genetic similarities between plant interactions with microbial pathogens and wheat interactions with Hessian fly larvae prompted us to investigate defense and counterdefense mechanisms. Plant oxidative burst, a rapid increase in the levels of active oxygen species (AOS) within the initial 24 h of an interaction with pathogens, commonly is associated with defenses that are triggered by gene-for-gene recognition events similar to those involving wheat and Hessian fly larvae. RNAs encoded by Hessian fly superoxide dismutase (SOD) and catalase (CAT) genes, involved in detoxification of AOS, increased in first-instar larvae during both compatible and incompatible interactions. However, mRNA levels of a wheat NADPH oxidase (NOX) gene that generates superoxide (O2-) did not increase. In addition, inhibiting wheat NOX enzyme with diphenyleneiodonium did not result in increased survival of avirulent larvae. However, nitro blue tetrazolium staining indicated that basal levels of O2- are present in both uninfested and infested wheat tissue. mRNA encoded by wheat genes involved in detoxification of the cellular environment, SOD, CAT, and glutathione-S-transferase did not increase in abundance. Histochemical staining with 3,3-diaminobenzidine revealed no increases in wheat hydrogen peroxide (H2O2) during infestation that were correlated with the changes in larval SOD and CAT mRNA. However, treatment with 2',7'-dichlorofluorescin demonstrated the presence of basal levels of H2O2 in the elongation zone of both infested and uninfested plants. The accumulation of a wheat flavanone 3-hydroxylase mRNA did show some parallels with larval gene mRNA profiles. These results suggested that larvae encounter stresses imposed by mechanisms other than an oxidative burst in wheat seedlings. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/16941906/Gene_for_gene_defense_of_wheat_against_the_Hessian_fly_lacks_a_classical_oxidative_burst_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1023?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -