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Human T cell receptor-gamma delta-expressing T-cell lines recognize MHC-controlled elements on autologous EBV-LCL that are not HLA-A, -B, -C, -DR, -DQ, or -DP.
J Immunol. 1990 Jul 01; 145(1):36-45.JI

Abstract

HLA-loss variants of an EBV-transformed B lymphoblastoid cell line (EBV-LCL) 721 were used to investigate whether human MHC molecules other than known class I or class II were involved in autologous T cell responses. Bulk lymphocyte cultures of purified T cells primed to an autologous variant EBV-LCL that fails to express HLA-class II and has reduced cell surface HLA-class I expression, and oligoclonal TCR-gamma delta-bearing lines derived from them, could lyse both this EBV-LCL and an independently derived, class II expressing autologous variant EBV-LCL that bears no HLA-A, -B, or -C, suggesting the presence of additional HLA-like restriction elements. Cold target inhibition of cytolysis mediated by these lines indicated that a shared or cross-reactive MHC controlled restriction element other than the known MHC determinants was retained by the EBV-LCL variants. Single-cell derived clones from these T cell lines which expressed only the TCR-gamma delta showed this same target cell specificity pattern, proving recognition of MHC-controlled determinants by autologous gamma delta T cells. Anti-gamma delta antibody could inhibit cytolysis by the gamma delta-expressing lines, suggesting that the TCR-gamma delta was involved in recognition of the EBV-LCL targets. Flow cytometric analysis with separate HLA-reactive antibodies indicated that the restriction element for these cytolytic responses is a molecule serologically cross-reactive with HLA-B and -C Ag, yet is a determinant that cannot be HLA-A, -B, -C, -DR, -DQ or -DP.

Authors+Show Affiliations

Department of Human Oncology, University of Wisconsin, Madison 53792.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1694208

Citation

Lam, V, et al. "Human T Cell Receptor-gamma Delta-expressing T-cell Lines Recognize MHC-controlled Elements On Autologous EBV-LCL That Are Not HLA-A, -B, -C, -DR, -DQ, or -DP." Journal of Immunology (Baltimore, Md. : 1950), vol. 145, no. 1, 1990, pp. 36-45.
Lam V, DeMars R, Chen BP, et al. Human T cell receptor-gamma delta-expressing T-cell lines recognize MHC-controlled elements on autologous EBV-LCL that are not HLA-A, -B, -C, -DR, -DQ, or -DP. J Immunol. 1990;145(1):36-45.
Lam, V., DeMars, R., Chen, B. P., Hank, J. A., Kovats, S., Fisch, P., & Sondel, P. M. (1990). Human T cell receptor-gamma delta-expressing T-cell lines recognize MHC-controlled elements on autologous EBV-LCL that are not HLA-A, -B, -C, -DR, -DQ, or -DP. Journal of Immunology (Baltimore, Md. : 1950), 145(1), 36-45.
Lam V, et al. Human T Cell Receptor-gamma Delta-expressing T-cell Lines Recognize MHC-controlled Elements On Autologous EBV-LCL That Are Not HLA-A, -B, -C, -DR, -DQ, or -DP. J Immunol. 1990 Jul 1;145(1):36-45. PubMed PMID: 1694208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human T cell receptor-gamma delta-expressing T-cell lines recognize MHC-controlled elements on autologous EBV-LCL that are not HLA-A, -B, -C, -DR, -DQ, or -DP. AU - Lam,V, AU - DeMars,R, AU - Chen,B P, AU - Hank,J A, AU - Kovats,S, AU - Fisch,P, AU - Sondel,P M, PY - 1990/7/1/pubmed PY - 1990/7/1/medline PY - 1990/7/1/entrez SP - 36 EP - 45 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 145 IS - 1 N2 - HLA-loss variants of an EBV-transformed B lymphoblastoid cell line (EBV-LCL) 721 were used to investigate whether human MHC molecules other than known class I or class II were involved in autologous T cell responses. Bulk lymphocyte cultures of purified T cells primed to an autologous variant EBV-LCL that fails to express HLA-class II and has reduced cell surface HLA-class I expression, and oligoclonal TCR-gamma delta-bearing lines derived from them, could lyse both this EBV-LCL and an independently derived, class II expressing autologous variant EBV-LCL that bears no HLA-A, -B, or -C, suggesting the presence of additional HLA-like restriction elements. Cold target inhibition of cytolysis mediated by these lines indicated that a shared or cross-reactive MHC controlled restriction element other than the known MHC determinants was retained by the EBV-LCL variants. Single-cell derived clones from these T cell lines which expressed only the TCR-gamma delta showed this same target cell specificity pattern, proving recognition of MHC-controlled determinants by autologous gamma delta T cells. Anti-gamma delta antibody could inhibit cytolysis by the gamma delta-expressing lines, suggesting that the TCR-gamma delta was involved in recognition of the EBV-LCL targets. Flow cytometric analysis with separate HLA-reactive antibodies indicated that the restriction element for these cytolytic responses is a molecule serologically cross-reactive with HLA-B and -C Ag, yet is a determinant that cannot be HLA-A, -B, -C, -DR, -DQ or -DP. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1694208/Human_T_cell_receptor_gamma_delta_expressing_T_cell_lines_recognize_MHC_controlled_elements_on_autologous_EBV_LCL_that_are_not_HLA_A__B__C__DR__DQ_or__DP_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1694208 DB - PRIME DP - Unbound Medicine ER -