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Non-specificity of nutritional substrate for ochratoxin A production by isolates of Aspergillus ochraceus.
Food Microbiol. 2006 Jun; 23(4):351-8.FM

Abstract

Aspergillus ochraceus is an important contaminant of diverse substrates, such as cereals, coffee, grapes and derivates. This fungus produce a nephrotoxic metabolite, ochratoxin A (OTA), whose presence on food and feeds may be an important risk for animal and human health. The aim of this work was to evaluate the significance of the origin of A. ochraceus isolates on their OTA production patterns on different substrates (yeast extract sucrose (YES) broth, irradiated barley grains, irradiated green coffee beans and sterilized grapes) and under different environmental conditions. Results did not show a significant influence of the isolation source on OTA-production profiles by A. ochraceus isolates on several substrates, since the isolates which produced the highest OTA amounts in vitro (YES medium) were also the isolates with the highest OTA yields on the other substrates. Abiotic factors assayed (water activity, temperature and substrate) affected significantly OTA productions by A. ochraceus. Maximum OTA amounts were detected at 25 degrees C and 0.98 a(w) on all substrates tested. The highest OTA accumulations found on the different substrates were: green coffee beans (> 2 mg g(-1)), barley grains (approximately 1 mg g(-1)), YES medium (13.9 microg ml(-1)) and grape (approximately 3 ng g(-1)).

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Publisher Full Text

Authors+Show Affiliations

Pardo E
Food Technology Department, University of Lleida, Rovira Roure 191, 25198 Lleida, Spain.
Sanchis V
No affiliation info available
Ramos AJ
No affiliation info available
Marín S
No affiliation info available

MeSH

Aspergillus ochraceusCoffeeCulture MediaEdible GrainFood ContaminationFood MicrobiologyModels, BiologicalOchratoxinsSubstrate SpecificityTemperatureVitisWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16943024

Citation

Pardo, E, et al. "Non-specificity of Nutritional Substrate for Ochratoxin a Production By Isolates of Aspergillus Ochraceus." Food Microbiology, vol. 23, no. 4, 2006, pp. 351-8.
Pardo E, Sanchis V, Ramos AJ, et al. Non-specificity of nutritional substrate for ochratoxin A production by isolates of Aspergillus ochraceus. Food Microbiol. 2006;23(4):351-8.
Pardo, E., Sanchis, V., Ramos, A. J., & Marín, S. (2006). Non-specificity of nutritional substrate for ochratoxin A production by isolates of Aspergillus ochraceus. Food Microbiology, 23(4), 351-8.
Pardo E, et al. Non-specificity of Nutritional Substrate for Ochratoxin a Production By Isolates of Aspergillus Ochraceus. Food Microbiol. 2006;23(4):351-8. PubMed PMID: 16943024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-specificity of nutritional substrate for ochratoxin A production by isolates of Aspergillus ochraceus. AU - Pardo,E, AU - Sanchis,V, AU - Ramos,A J, AU - Marín,S, Y1 - 2005/07/07/ PY - 2005/02/09/received PY - 2005/05/12/revised PY - 2005/05/12/accepted PY - 2006/9/1/pubmed PY - 2006/9/26/medline PY - 2006/9/1/entrez SP - 351 EP - 8 JF - Food microbiology JO - Food Microbiol VL - 23 IS - 4 N2 - Aspergillus ochraceus is an important contaminant of diverse substrates, such as cereals, coffee, grapes and derivates. This fungus produce a nephrotoxic metabolite, ochratoxin A (OTA), whose presence on food and feeds may be an important risk for animal and human health. The aim of this work was to evaluate the significance of the origin of A. ochraceus isolates on their OTA production patterns on different substrates (yeast extract sucrose (YES) broth, irradiated barley grains, irradiated green coffee beans and sterilized grapes) and under different environmental conditions. Results did not show a significant influence of the isolation source on OTA-production profiles by A. ochraceus isolates on several substrates, since the isolates which produced the highest OTA amounts in vitro (YES medium) were also the isolates with the highest OTA yields on the other substrates. Abiotic factors assayed (water activity, temperature and substrate) affected significantly OTA productions by A. ochraceus. Maximum OTA amounts were detected at 25 degrees C and 0.98 a(w) on all substrates tested. The highest OTA accumulations found on the different substrates were: green coffee beans (> 2 mg g(-1)), barley grains (approximately 1 mg g(-1)), YES medium (13.9 microg ml(-1)) and grape (approximately 3 ng g(-1)). SN - 0740-0020 UR - https://www.unboundmedicine.com/medline/citation/16943024/Non_specificity_of_nutritional_substrate_for_ochratoxin_A_production_by_isolates_of_Aspergillus_ochraceus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(05)00069-9 DB - PRIME DP - Unbound Medicine ER -