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Effects of a cannabinoid agonist on spinal nociceptive neurons in a rodent model of neuropathic pain.
J Neurophysiol 2006; 96(6):2984-94JN

Abstract

The effects of the synthetic cannabinoid WIN 55,212-2 on heat-evoked firing of spinal wide dynamic range (WDR) neurons were examined in a rodent model of neuropathic pain. Fifty-eight WDR neurons (1 cell/animal) were recorded from the ipsilateral spinal dorsal horns of rats with chronic constriction injury (CCI) and sham-operated controls. Relative to sham-operated controls, neurons recorded in CCI rats showed elevations in spontaneous firing, noxious heat-evoked responses, and afterdischarge firing as well as increases in receptive field size. WIN 55,212-2 (0.0625, 0.125, and 0.25 mg/kg, intravenous) dose-dependently suppressed heat-evoked activity and decreased the receptive field areas of dorsal horn WDR neurons in both nerve injured and control rats with a greater inhibition in CCI rats. At the dose of 0.125 mg/kg iv, WIN 55,212-2 reversed the hyperalgesia produced by nerve injury. The effect of intravenous administration of WIN 55,212-2 appears to be centrally mediated because administration of the drug directly to the ligated nerve did not suppress the heat-evoked neuronal activity in CCI rats. Pretreatment with the cannabinoid CB(1) receptor antagonists SR141716A or AM251, but not the CB(2) antagonist SR144528, blocked the effects. These results provide a neural basis for reports of potent suppression by cannabinoids of the abnormal sensory responses that result from nerve injury.

Authors+Show Affiliations

Department of Psychological and Brain Sciences, Indiana University, 1101 E. 10th Street, Bloomington, IN 47405-7007, USA. walkerjm@indiana.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16943316

Citation

Liu, Cheng, and J Michael Walker. "Effects of a Cannabinoid Agonist On Spinal Nociceptive Neurons in a Rodent Model of Neuropathic Pain." Journal of Neurophysiology, vol. 96, no. 6, 2006, pp. 2984-94.
Liu C, Walker JM. Effects of a cannabinoid agonist on spinal nociceptive neurons in a rodent model of neuropathic pain. J Neurophysiol. 2006;96(6):2984-94.
Liu, C., & Walker, J. M. (2006). Effects of a cannabinoid agonist on spinal nociceptive neurons in a rodent model of neuropathic pain. Journal of Neurophysiology, 96(6), pp. 2984-94.
Liu C, Walker JM. Effects of a Cannabinoid Agonist On Spinal Nociceptive Neurons in a Rodent Model of Neuropathic Pain. J Neurophysiol. 2006;96(6):2984-94. PubMed PMID: 16943316.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a cannabinoid agonist on spinal nociceptive neurons in a rodent model of neuropathic pain. AU - Liu,Cheng, AU - Walker,J Michael, Y1 - 2006/08/30/ PY - 2006/9/1/pubmed PY - 2007/1/18/medline PY - 2006/9/1/entrez SP - 2984 EP - 94 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 96 IS - 6 N2 - The effects of the synthetic cannabinoid WIN 55,212-2 on heat-evoked firing of spinal wide dynamic range (WDR) neurons were examined in a rodent model of neuropathic pain. Fifty-eight WDR neurons (1 cell/animal) were recorded from the ipsilateral spinal dorsal horns of rats with chronic constriction injury (CCI) and sham-operated controls. Relative to sham-operated controls, neurons recorded in CCI rats showed elevations in spontaneous firing, noxious heat-evoked responses, and afterdischarge firing as well as increases in receptive field size. WIN 55,212-2 (0.0625, 0.125, and 0.25 mg/kg, intravenous) dose-dependently suppressed heat-evoked activity and decreased the receptive field areas of dorsal horn WDR neurons in both nerve injured and control rats with a greater inhibition in CCI rats. At the dose of 0.125 mg/kg iv, WIN 55,212-2 reversed the hyperalgesia produced by nerve injury. The effect of intravenous administration of WIN 55,212-2 appears to be centrally mediated because administration of the drug directly to the ligated nerve did not suppress the heat-evoked neuronal activity in CCI rats. Pretreatment with the cannabinoid CB(1) receptor antagonists SR141716A or AM251, but not the CB(2) antagonist SR144528, blocked the effects. These results provide a neural basis for reports of potent suppression by cannabinoids of the abnormal sensory responses that result from nerve injury. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/16943316/Effects_of_a_cannabinoid_agonist_on_spinal_nociceptive_neurons_in_a_rodent_model_of_neuropathic_pain_ L2 - http://www.physiology.org/doi/full/10.1152/jn.00498.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -