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Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.).
C R Biol. 2006 Sep; 329(9):719-25.CR

Abstract

Phosphoenolpyruvate carboxylase (PEPc: EC 4.1.1.31) activity was monitored during seed maturation of two varieties (Hybridol and Pactol) of rapeseed (Brassica napus L.), widely cultivated in Tunisia. In the Hybridol variety, PEPc activity did not exceed 5 micromol h(-1) per gram of fresh weight (FW) during the first stages of maturation. It then highly increased to reach more than 30 micromol h(-1) g(-1)/FW. On the contrary, in the Pactol variety, the evolution of PEPc activity showed a classical curve, i.e. an increase during the most active phase of lipid accumulation in maturating seeds, followed by a rapid decrease until the end of seed maturation. In both varieties, the seed oil was characterised by a high content of oleic acid (C(18:1)), linoleic (C(18:2)) and linolenic acids (C(18:3)). Saturated fatty acids were also present, although decreasing with maturation course. The analysis of the triacylglycerols (TAG) showed that trioleoylglycerol (OOO) and dioleoyllinoleoylglycerol (OOL) were the major species (ca. 35% and ca. 25% of the total respectively). The evolution pattern of fatty acids and TAG contents was similar to that of PEPc activity. Taken together, our findings suggest that PEPc may be involved in fatty acid and triacylglycerol biosynthesis during seed maturation of both rapeseed varieties.

Links

Publisher Full Text
linkinghub.elsevier.com
linkinghub.elsevier.com

Authors+Show Affiliations

Sebei K
Laboratoire de Biochimie des Lipides et Interactions avec les Macromolécules, Faculté des Sciences de Tunis, Université de Tunis-El-Manar, campus universitaire, BP 187, PTT Elmanar, 2092 Tunis, Tunisia. khaledshtn@yahoo.fr
Ouerghi Z
No affiliation info available
Kallel H
No affiliation info available
Boukhchina S
No affiliation info available

MeSH

Brassica rapaFatty AcidsLinoleic AcidLipidsOleic AcidPhosphoenolpyruvate CarboxylasePlant OilsSeedsSpecies SpecificityTriglyceridesTunisiaalpha-Linolenic Acid

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16945838

Citation

Sebei, Khaled, et al. "Evolution of Phosphoenolpyruvate Carboxylase Activity and Lipid Content During Seed Maturation of Two Spring Rapeseed Cultivars (Brassica Napus L.)." Comptes Rendus Biologies, vol. 329, no. 9, 2006, pp. 719-25.
Sebei K, Ouerghi Z, Kallel H, et al. Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.). C R Biol. 2006;329(9):719-25.
Sebei, K., Ouerghi, Z., Kallel, H., & Boukhchina, S. (2006). Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.). Comptes Rendus Biologies, 329(9), 719-25.
Sebei K, et al. Evolution of Phosphoenolpyruvate Carboxylase Activity and Lipid Content During Seed Maturation of Two Spring Rapeseed Cultivars (Brassica Napus L.). C R Biol. 2006;329(9):719-25. PubMed PMID: 16945838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.). AU - Sebei,Khaled, AU - Ouerghi,Zeineb, AU - Kallel,Habib, AU - Boukhchina,Sadok, Y1 - 2006/07/14/ PY - 2005/10/21/received PY - 2006/06/12/revised PY - 2006/06/14/accepted PY - 2006/9/2/pubmed PY - 2006/11/4/medline PY - 2006/9/2/entrez SP - 719 EP - 25 JF - Comptes rendus biologies JO - C R Biol VL - 329 IS - 9 N2 - Phosphoenolpyruvate carboxylase (PEPc: EC 4.1.1.31) activity was monitored during seed maturation of two varieties (Hybridol and Pactol) of rapeseed (Brassica napus L.), widely cultivated in Tunisia. In the Hybridol variety, PEPc activity did not exceed 5 micromol h(-1) per gram of fresh weight (FW) during the first stages of maturation. It then highly increased to reach more than 30 micromol h(-1) g(-1)/FW. On the contrary, in the Pactol variety, the evolution of PEPc activity showed a classical curve, i.e. an increase during the most active phase of lipid accumulation in maturating seeds, followed by a rapid decrease until the end of seed maturation. In both varieties, the seed oil was characterised by a high content of oleic acid (C(18:1)), linoleic (C(18:2)) and linolenic acids (C(18:3)). Saturated fatty acids were also present, although decreasing with maturation course. The analysis of the triacylglycerols (TAG) showed that trioleoylglycerol (OOO) and dioleoyllinoleoylglycerol (OOL) were the major species (ca. 35% and ca. 25% of the total respectively). The evolution pattern of fatty acids and TAG contents was similar to that of PEPc activity. Taken together, our findings suggest that PEPc may be involved in fatty acid and triacylglycerol biosynthesis during seed maturation of both rapeseed varieties. SN - 1631-0691 UR - https://www.unboundmedicine.com/medline/citation/16945838/Evolution_of_phosphoenolpyruvate_carboxylase_activity_and_lipid_content_during_seed_maturation_of_two_spring_rapeseed_cultivars__Brassica_napus_L___ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1631-0691(06)00156-9 DB - PRIME DP - Unbound Medicine ER -