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Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits.
J Allergy Clin Immunol. 2006 Sep; 118(3):711-8.JA

Abstract

BACKGROUND

Today, for patients with food allergy, the only possibility to prevent allergic reactions is avoidance of the allergenic food. Genetic engineering of hypoallergenic plants by means of RNA interference (RNAi) could be an approach to improve the quality of life of subjects with food allergy.

OBJECTIVES

We sought to achieve stable inhibition of expression of the allergenic nonspecific lipid transfer protein Lyc e 3 in tomato and to analyze the reduction of allergenicity in vitro by using histamine release assays and in vivo by using skin prick tests with transgenic tomato fruits.

METHODS

Gene silencing was performed by means of RNAi and monitored by using Western blotting with nonspecific lipid transfer protein-specific antibodies and sera from patients with tomato allergy. Dose-dependent basophil histamine release assays, prick-to-prick skin testing, and determination of endogenous histamine content were performed with fruits harvested from plants of the first and second generation to assess the allergenic potency compared with that of wild-type fruits.

RESULTS

We demonstrated that silencing of Lyc e 3 by means of RNAi contributes to reduced skin reactivity and is passed on to the next generation of fruits. A significant reduction of allergenic potency was determined in vitro and confirmed by using skin prick tests.

CONCLUSION

Taken together, these results indicate that RNAi technology is an effective tool to generate foods with reduced allergenicity.

CLINICAL IMPLICATIONS

Allergen-reduced plant foods might allow reduction of dietary restrictions for patients allergic to panallergen families.

Authors+Show Affiliations

Paul-Ehrlich-Institut, Langen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16950292

Citation

Lorenz, Yvonne, et al. "Skin Prick Tests Reveal Stable and Heritable Reduction of Allergenic Potency of Gene-silenced Tomato Fruits." The Journal of Allergy and Clinical Immunology, vol. 118, no. 3, 2006, pp. 711-8.
Lorenz Y, Enrique E, Lequynh L, et al. Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits. J Allergy Clin Immunol. 2006;118(3):711-8.
Lorenz, Y., Enrique, E., Lequynh, L., Fötisch, K., Retzek, M., Biemelt, S., Sonnewald, U., Vieths, S., & Scheurer, S. (2006). Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits. The Journal of Allergy and Clinical Immunology, 118(3), 711-8.
Lorenz Y, et al. Skin Prick Tests Reveal Stable and Heritable Reduction of Allergenic Potency of Gene-silenced Tomato Fruits. J Allergy Clin Immunol. 2006;118(3):711-8. PubMed PMID: 16950292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits. AU - Lorenz,Yvonne, AU - Enrique,Ernesto, AU - Lequynh,Lien, AU - Fötisch,Kay, AU - Retzek,Mechthild, AU - Biemelt,Sophia, AU - Sonnewald,Uwe, AU - Vieths,Stefan, AU - Scheurer,Stephan, Y1 - 2006/07/12/ PY - 2006/02/27/received PY - 2006/04/12/revised PY - 2006/05/18/accepted PY - 2006/9/5/pubmed PY - 2006/10/18/medline PY - 2006/9/5/entrez SP - 711 EP - 8 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 118 IS - 3 N2 - BACKGROUND: Today, for patients with food allergy, the only possibility to prevent allergic reactions is avoidance of the allergenic food. Genetic engineering of hypoallergenic plants by means of RNA interference (RNAi) could be an approach to improve the quality of life of subjects with food allergy. OBJECTIVES: We sought to achieve stable inhibition of expression of the allergenic nonspecific lipid transfer protein Lyc e 3 in tomato and to analyze the reduction of allergenicity in vitro by using histamine release assays and in vivo by using skin prick tests with transgenic tomato fruits. METHODS: Gene silencing was performed by means of RNAi and monitored by using Western blotting with nonspecific lipid transfer protein-specific antibodies and sera from patients with tomato allergy. Dose-dependent basophil histamine release assays, prick-to-prick skin testing, and determination of endogenous histamine content were performed with fruits harvested from plants of the first and second generation to assess the allergenic potency compared with that of wild-type fruits. RESULTS: We demonstrated that silencing of Lyc e 3 by means of RNAi contributes to reduced skin reactivity and is passed on to the next generation of fruits. A significant reduction of allergenic potency was determined in vitro and confirmed by using skin prick tests. CONCLUSION: Taken together, these results indicate that RNAi technology is an effective tool to generate foods with reduced allergenicity. CLINICAL IMPLICATIONS: Allergen-reduced plant foods might allow reduction of dietary restrictions for patients allergic to panallergen families. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/16950292/Skin_prick_tests_reveal_stable_and_heritable_reduction_of_allergenic_potency_of_gene_silenced_tomato_fruits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(06)01189-4 DB - PRIME DP - Unbound Medicine ER -