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Voltammetry and quantification of the contraceptive drug norethisterone in bulk form and pharmaceutical formulation.
J Pharm Biomed Anal. 2007 Jan 17; 43(2):499-505.JP

Abstract

The electrochemical behavior of norethisterone at the mercury electrode was studied in the universal buffer of various pH values using dc-polarography, cyclic voltammetry and controlled-potential electrolysis. Norethisterone was reduced at the mercury electrode via the consumption of two electrons corresponding to reduction of the 3-keto-delta-4-group in the A-ring of the molecule. The pK(a) value (8.7) of norethisterone was determined from the polarographic and spectrophotometric measurements. A fully validated, simple, sensitive, precise and inexpensive square-wave adsorptive cathodic stripping (SWAdCS) voltammetry procedure was described for trace quantification of bulk norethisterone. The stripping voltammetry peak current of norethisterone in a universal buffer of pH 5 following its accumulation onto the hanging mercury drop electrode (HMDE) at -0.6 V (versus Ag/AgCl/KCl(s)) for 130 s showed a linear response with the concentration over the range 5 x 10(-9) to 3 x 10(-7)M norethisterone. Detection and quantitation limits of 1.5 x 10(-9) and 5 x 10(-9)M bulk norethisterone, respectively, were achieved. The proposed procedure was successfully applied for the assay of norethisterone in Steronate tablets without interference from excipients.

Authors+Show Affiliations

Chemistry Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt. mmghoneim@usa.netNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

16950587

Citation

Ghoneim, M M., et al. "Voltammetry and Quantification of the Contraceptive Drug Norethisterone in Bulk Form and Pharmaceutical Formulation." Journal of Pharmaceutical and Biomedical Analysis, vol. 43, no. 2, 2007, pp. 499-505.
Ghoneim MM, Abushoffa AM, Moharram YI, et al. Voltammetry and quantification of the contraceptive drug norethisterone in bulk form and pharmaceutical formulation. J Pharm Biomed Anal. 2007;43(2):499-505.
Ghoneim, M. M., Abushoffa, A. M., Moharram, Y. I., & El-Desoky, H. S. (2007). Voltammetry and quantification of the contraceptive drug norethisterone in bulk form and pharmaceutical formulation. Journal of Pharmaceutical and Biomedical Analysis, 43(2), 499-505.
Ghoneim MM, et al. Voltammetry and Quantification of the Contraceptive Drug Norethisterone in Bulk Form and Pharmaceutical Formulation. J Pharm Biomed Anal. 2007 Jan 17;43(2):499-505. PubMed PMID: 16950587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Voltammetry and quantification of the contraceptive drug norethisterone in bulk form and pharmaceutical formulation. AU - Ghoneim,M M, AU - Abushoffa,A M, AU - Moharram,Y I, AU - El-Desoky,H S, Y1 - 2006/09/06/ PY - 2006/03/26/received PY - 2006/07/26/revised PY - 2006/07/27/accepted PY - 2006/9/5/pubmed PY - 2007/7/10/medline PY - 2006/9/5/entrez SP - 499 EP - 505 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 43 IS - 2 N2 - The electrochemical behavior of norethisterone at the mercury electrode was studied in the universal buffer of various pH values using dc-polarography, cyclic voltammetry and controlled-potential electrolysis. Norethisterone was reduced at the mercury electrode via the consumption of two electrons corresponding to reduction of the 3-keto-delta-4-group in the A-ring of the molecule. The pK(a) value (8.7) of norethisterone was determined from the polarographic and spectrophotometric measurements. A fully validated, simple, sensitive, precise and inexpensive square-wave adsorptive cathodic stripping (SWAdCS) voltammetry procedure was described for trace quantification of bulk norethisterone. The stripping voltammetry peak current of norethisterone in a universal buffer of pH 5 following its accumulation onto the hanging mercury drop electrode (HMDE) at -0.6 V (versus Ag/AgCl/KCl(s)) for 130 s showed a linear response with the concentration over the range 5 x 10(-9) to 3 x 10(-7)M norethisterone. Detection and quantitation limits of 1.5 x 10(-9) and 5 x 10(-9)M bulk norethisterone, respectively, were achieved. The proposed procedure was successfully applied for the assay of norethisterone in Steronate tablets without interference from excipients. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16950587/Voltammetry_and_quantification_of_the_contraceptive_drug_norethisterone_in_bulk_form_and_pharmaceutical_formulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(06)00520-6 DB - PRIME DP - Unbound Medicine ER -