Tags

Type your tag names separated by a space and hit enter

Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes.
J Clin Microbiol 2006; 44(9):3352-60JC

Abstract

Bacillus cereus is ubiquitous in nature, and while most isolates appear to be harmless, some are associated with food-borne illnesses, periodontal diseases, and other more serious infections. In one such infection, B. cereus G9241 was identified as the causative agent of a severe pneumonia in a Louisiana welder in 1994. This isolate was found to harbor most of the B. anthracis virulence plasmid pXO1 (13). Here we report the characterization of two clinical and one environmental B. cereus isolate collected during an investigation of two fatal pneumonia cases in Texas metal workers. Molecular subtyping revealed that the two cases were not caused by the same strain. However, one of the three isolates was indistinguishable from B. cereus G9241. PCR analysis demonstrated that both clinical isolates contained B. anthracis pXO1 toxin genes. One clinical isolate and the environmental isolate collected from that victim's worksite contained the cap A, B, and C genes required for capsule biosynthesis in B. anthracis. Both clinical isolates expressed a capsule; however, neither was composed of poly-D-glutamic acid. Although most B. cereus isolates are not opportunistic pathogens and only a limited number cause food-borne illnesses, these results demonstrate that some B. cereus strains can cause severe and even fatal infections in patients who appear to be otherwise healthy.

Authors+Show Affiliations

Epidemiologic Investigations Laboratory, Meningitis and Special Pathogens Branch, MS G34, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333, USA. amh9@cdc.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16954272

Citation

Hoffmaster, Alex R., et al. "Characterization of Bacillus Cereus Isolates Associated With Fatal Pneumonias: Strains Are Closely Related to Bacillus Anthracis and Harbor B. Anthracis Virulence Genes." Journal of Clinical Microbiology, vol. 44, no. 9, 2006, pp. 3352-60.
Hoffmaster AR, Hill KK, Gee JE, et al. Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes. J Clin Microbiol. 2006;44(9):3352-60.
Hoffmaster, A. R., Hill, K. K., Gee, J. E., Marston, C. K., De, B. K., Popovic, T., ... Jackson, P. J. (2006). Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes. Journal of Clinical Microbiology, 44(9), pp. 3352-60.
Hoffmaster AR, et al. Characterization of Bacillus Cereus Isolates Associated With Fatal Pneumonias: Strains Are Closely Related to Bacillus Anthracis and Harbor B. Anthracis Virulence Genes. J Clin Microbiol. 2006;44(9):3352-60. PubMed PMID: 16954272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes. AU - Hoffmaster,Alex R, AU - Hill,Karen K, AU - Gee,Jay E, AU - Marston,Chung K, AU - De,Barun K, AU - Popovic,Tanja, AU - Sue,David, AU - Wilkins,Patricia P, AU - Avashia,Swati B, AU - Drumgoole,Rahsaan, AU - Helma,Charles H, AU - Ticknor,Lawrence O, AU - Okinaka,Richard T, AU - Jackson,Paul J, PY - 2006/9/7/pubmed PY - 2006/10/28/medline PY - 2006/9/7/entrez SP - 3352 EP - 60 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 44 IS - 9 N2 - Bacillus cereus is ubiquitous in nature, and while most isolates appear to be harmless, some are associated with food-borne illnesses, periodontal diseases, and other more serious infections. In one such infection, B. cereus G9241 was identified as the causative agent of a severe pneumonia in a Louisiana welder in 1994. This isolate was found to harbor most of the B. anthracis virulence plasmid pXO1 (13). Here we report the characterization of two clinical and one environmental B. cereus isolate collected during an investigation of two fatal pneumonia cases in Texas metal workers. Molecular subtyping revealed that the two cases were not caused by the same strain. However, one of the three isolates was indistinguishable from B. cereus G9241. PCR analysis demonstrated that both clinical isolates contained B. anthracis pXO1 toxin genes. One clinical isolate and the environmental isolate collected from that victim's worksite contained the cap A, B, and C genes required for capsule biosynthesis in B. anthracis. Both clinical isolates expressed a capsule; however, neither was composed of poly-D-glutamic acid. Although most B. cereus isolates are not opportunistic pathogens and only a limited number cause food-borne illnesses, these results demonstrate that some B. cereus strains can cause severe and even fatal infections in patients who appear to be otherwise healthy. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/16954272/Characterization_of_Bacillus_cereus_isolates_associated_with_fatal_pneumonias:_strains_are_closely_related_to_Bacillus_anthracis_and_harbor_B__anthracis_virulence_genes_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=16954272 DB - PRIME DP - Unbound Medicine ER -