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Modulation of histamine release by rat peritoneal mast cells in a superfusion set-up.
Agents Actions. 1990 Apr; 30(1-2):34-7.AA

Abstract

A new method is presented for studying mediator release in isolated peritoneal cells of the rat. Using a superfusion technique, cells can be maintained in a continuously cleared medium. Cells were stimulated with antigen, compound 48/80 or A23187 to characterize this preparation. In addition, inhibitory effects of theophylline, isoprenaline and cromoglycate on compound 48/80-induced histamine release were studied. We conclude that superfusion is a valid alternative to study mediator release from isolated peritoneal cells.

Authors+Show Affiliations

Post MJ
Department of Pharmacotherapy, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Moekoet C
No affiliation info available
Wemer J
No affiliation info available
van Rooij HH
No affiliation info available
Porsius AJ
No affiliation info available

MeSH

AnimalsAntigensCalcimycinCromolyn SodiumHistamine ReleaseIn Vitro TechniquesIsoproterenolMaleMast CellsPeritoneal CavityRatsRats, Inbred StrainsTheophyllinep-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1695454

Citation

Post, M J., et al. "Modulation of Histamine Release By Rat Peritoneal Mast Cells in a Superfusion Set-up." Agents and Actions, vol. 30, no. 1-2, 1990, pp. 34-7.
Post MJ, Moekoet C, Wemer J, et al. Modulation of histamine release by rat peritoneal mast cells in a superfusion set-up. Agents Actions. 1990;30(1-2):34-7.
Post, M. J., Moekoet, C., Wemer, J., van Rooij, H. H., & Porsius, A. J. (1990). Modulation of histamine release by rat peritoneal mast cells in a superfusion set-up. Agents and Actions, 30(1-2), 34-7.
Post MJ, et al. Modulation of Histamine Release By Rat Peritoneal Mast Cells in a Superfusion Set-up. Agents Actions. 1990;30(1-2):34-7. PubMed PMID: 1695454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of histamine release by rat peritoneal mast cells in a superfusion set-up. AU - Post,M J, AU - Moekoet,C, AU - Wemer,J, AU - van Rooij,H H, AU - Porsius,A J, PY - 1990/4/1/pubmed PY - 1990/4/1/medline PY - 1990/4/1/entrez SP - 34 EP - 7 JF - Agents and actions JO - Agents Actions VL - 30 IS - 1-2 N2 - A new method is presented for studying mediator release in isolated peritoneal cells of the rat. Using a superfusion technique, cells can be maintained in a continuously cleared medium. Cells were stimulated with antigen, compound 48/80 or A23187 to characterize this preparation. In addition, inhibitory effects of theophylline, isoprenaline and cromoglycate on compound 48/80-induced histamine release were studied. We conclude that superfusion is a valid alternative to study mediator release from isolated peritoneal cells. SN - 0065-4299 UR - https://www.unboundmedicine.com/medline/citation/1695454/Modulation_of_histamine_release_by_rat_peritoneal_mast_cells_in_a_superfusion_set_up_ DB - PRIME DP - Unbound Medicine ER -