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A mechanism of formation of a persistent hybrid between elongating RNA and template DNA.
Cell. 1990 Jul 27; 62(2):331-8.Cell

Abstract

At the replication origin of ColE1 plasmid, a persistent hybrid is formed between the primer precursor (RNA II) and its template DNA. The wild-type sequence in the region 13 to 20 bp upstream (-20 region) of the origin is required to form this persistent hybrid. While the template strand for transcription of this region, containing a stretch of six dC residues, is needed, the nontemplate strand can be deleted. Certain mutations in far upstream regions that prevent hybrid formation are suppressed by the nontemplate strand deletion. In RNA II that is forming a persistent hybrid, the region about 265 nucleotides upstream of the origin (-265 region) can also form a hybrid with the template DNA. The -265 region of RNA II that consists of a stretch of six rG residues probably interacts with the dC stretch of the -20 region in the template strand to promote hybrid formation.

Authors+Show Affiliations

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1695550

Citation

Masukata, H, and J Tomizawa. "A Mechanism of Formation of a Persistent Hybrid Between Elongating RNA and Template DNA." Cell, vol. 62, no. 2, 1990, pp. 331-8.
Masukata H, Tomizawa J. A mechanism of formation of a persistent hybrid between elongating RNA and template DNA. Cell. 1990;62(2):331-8.
Masukata, H., & Tomizawa, J. (1990). A mechanism of formation of a persistent hybrid between elongating RNA and template DNA. Cell, 62(2), 331-8.
Masukata H, Tomizawa J. A Mechanism of Formation of a Persistent Hybrid Between Elongating RNA and Template DNA. Cell. 1990 Jul 27;62(2):331-8. PubMed PMID: 1695550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mechanism of formation of a persistent hybrid between elongating RNA and template DNA. AU - Masukata,H, AU - Tomizawa,J, PY - 1990/7/27/pubmed PY - 1990/7/27/medline PY - 1990/7/27/entrez SP - 331 EP - 8 JF - Cell JO - Cell VL - 62 IS - 2 N2 - At the replication origin of ColE1 plasmid, a persistent hybrid is formed between the primer precursor (RNA II) and its template DNA. The wild-type sequence in the region 13 to 20 bp upstream (-20 region) of the origin is required to form this persistent hybrid. While the template strand for transcription of this region, containing a stretch of six dC residues, is needed, the nontemplate strand can be deleted. Certain mutations in far upstream regions that prevent hybrid formation are suppressed by the nontemplate strand deletion. In RNA II that is forming a persistent hybrid, the region about 265 nucleotides upstream of the origin (-265 region) can also form a hybrid with the template DNA. The -265 region of RNA II that consists of a stretch of six rG residues probably interacts with the dC stretch of the -20 region in the template strand to promote hybrid formation. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/1695550/A_mechanism_of_formation_of_a_persistent_hybrid_between_elongating_RNA_and_template_DNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(90)90370-T DB - PRIME DP - Unbound Medicine ER -