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The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves.
J Exp Bot. 2006; 57(14):3619-26.JE

Abstract

The barley (Hordeum vulgare) gene Hv.AGP.S.1 produces two different transcripts encoding small subunits (SSUs) of ADP-glucose pyrophosphorylase (AGPase). It was shown previously that one of these transcripts, Hv.1a, encodes the cytosolic SSU in the endosperm. It is shown here that the other transcript produced from Hv.AGP.S.1, Hv.1b, encodes a plastidial SSU that is required for >90% of the AGPase activity in the leaves. Thus, both of the alternative transcripts encoded by Hv.AGP.S.1 are physiologically relevant: One is important for starch synthesis in the endosperm and the other for starch synthesis in the leaves. Although the Hv.1b transcript is abundant in embryos and present in endosperm, there is no evidence that a protein is produced from this transcript in these organs. This suggests that some, as yet unidentified, post-transcriptional control mechanism prevents the accumulation of the protein encoded by Hv.1b in embryos and endosperm but not in leaves. There is one other known gene in barley, Hv.AGP.S.2, encoding a SSU of AGPase. This gene has been shown to be responsible for the plastidial SSU in the endosperm. It is shown here that Hv.AGP.S.2 probably also makes some contribution to the SSU of AGPase in the leaves and may be responsible for most or all of the plastidial SSU in a range of non-photosynthetic plant organs including the embryo.

Authors+Show Affiliations

John Innes Centre, Norwich Research Park, Norfolk NR4 7UH, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16957017

Citation

Rösti, Sandrine, et al. "The Gene Encoding the Cytosolic Small Subunit of ADP-glucose Pyrophosphorylase in Barley Endosperm Also Encodes the Major Plastidial Small Subunit in the Leaves." Journal of Experimental Botany, vol. 57, no. 14, 2006, pp. 3619-26.
Rösti S, Rudi H, Rudi K, et al. The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves. J Exp Bot. 2006;57(14):3619-26.
Rösti, S., Rudi, H., Rudi, K., Opsahl-Sorteberg, H. G., Fahy, B., & Denyer, K. (2006). The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves. Journal of Experimental Botany, 57(14), 3619-26.
Rösti S, et al. The Gene Encoding the Cytosolic Small Subunit of ADP-glucose Pyrophosphorylase in Barley Endosperm Also Encodes the Major Plastidial Small Subunit in the Leaves. J Exp Bot. 2006;57(14):3619-26. PubMed PMID: 16957017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves. AU - Rösti,Sandrine, AU - Rudi,Heidi, AU - Rudi,Knut, AU - Opsahl-Sorteberg,Hilde-Gunn, AU - Fahy,Brendan, AU - Denyer,Kay, Y1 - 2006/09/06/ PY - 2006/9/8/pubmed PY - 2007/2/28/medline PY - 2006/9/8/entrez SP - 3619 EP - 26 JF - Journal of experimental botany JO - J Exp Bot VL - 57 IS - 14 N2 - The barley (Hordeum vulgare) gene Hv.AGP.S.1 produces two different transcripts encoding small subunits (SSUs) of ADP-glucose pyrophosphorylase (AGPase). It was shown previously that one of these transcripts, Hv.1a, encodes the cytosolic SSU in the endosperm. It is shown here that the other transcript produced from Hv.AGP.S.1, Hv.1b, encodes a plastidial SSU that is required for >90% of the AGPase activity in the leaves. Thus, both of the alternative transcripts encoded by Hv.AGP.S.1 are physiologically relevant: One is important for starch synthesis in the endosperm and the other for starch synthesis in the leaves. Although the Hv.1b transcript is abundant in embryos and present in endosperm, there is no evidence that a protein is produced from this transcript in these organs. This suggests that some, as yet unidentified, post-transcriptional control mechanism prevents the accumulation of the protein encoded by Hv.1b in embryos and endosperm but not in leaves. There is one other known gene in barley, Hv.AGP.S.2, encoding a SSU of AGPase. This gene has been shown to be responsible for the plastidial SSU in the endosperm. It is shown here that Hv.AGP.S.2 probably also makes some contribution to the SSU of AGPase in the leaves and may be responsible for most or all of the plastidial SSU in a range of non-photosynthetic plant organs including the embryo. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/16957017/The_gene_encoding_the_cytosolic_small_subunit_of_ADP_glucose_pyrophosphorylase_in_barley_endosperm_also_encodes_the_major_plastidial_small_subunit_in_the_leaves_ DB - PRIME DP - Unbound Medicine ER -