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Lettuce for human consumption collected in Costa Rica contains complex communities of culturable oxytetracycline- and gentamicin-resistant bacteria.
Appl Environ Microbiol 2006; 72(9):5870-6AE

Abstract

The present widespread use of antimicrobials in crop farming is based upon their successful application in human medicine. However, recent evidence suggests that the massive anthropogenic release of antimicrobials into the biosphere has selected for resistant bacteria and facilitated the transfer of resistance genes among them. This work deals with the examination of iceberg lettuce collected at 10 farms from two regions in Costa Rica. Farmers from nine sampling sites regularly apply commercial formulations containing gentamicin, oxytetracycline, streptomycin, or a combination of them without being able to indicate how often and how much of these products have been sprayed onto the crops. One organic farm was also investigated for comparative purposes. Oxytetracycline- and gentamicin-resistant bacteria were abundantly detected using selective enrichment cultures. Furthermore, colony mixtures from selective plates were characterized by chemotaxonomical and molecular fingerprinting methods. Both types of resistant communities accounted for a significant fraction of all culturable bacteria and included several resistance genes as well as factors for their potential horizontal transfer. Given the fact that lettuce is eaten raw, it may contribute to the dissemination of antimicrobial-resistant bacteria and/or their resistance genes from the environment to the microbial biota of the human intestine.

Authors+Show Affiliations

Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastrasse 11, D-49069 Osnabrück, Germany. rodriguez@biologie.uni-osnabrueck.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16957206

Citation

Rodríguez, César, et al. "Lettuce for Human Consumption Collected in Costa Rica Contains Complex Communities of Culturable Oxytetracycline- and Gentamicin-resistant Bacteria." Applied and Environmental Microbiology, vol. 72, no. 9, 2006, pp. 5870-6.
Rodríguez C, Lang L, Wang A, et al. Lettuce for human consumption collected in Costa Rica contains complex communities of culturable oxytetracycline- and gentamicin-resistant bacteria. Appl Environ Microbiol. 2006;72(9):5870-6.
Rodríguez, C., Lang, L., Wang, A., Altendorf, K., García, F., & Lipski, A. (2006). Lettuce for human consumption collected in Costa Rica contains complex communities of culturable oxytetracycline- and gentamicin-resistant bacteria. Applied and Environmental Microbiology, 72(9), pp. 5870-6.
Rodríguez C, et al. Lettuce for Human Consumption Collected in Costa Rica Contains Complex Communities of Culturable Oxytetracycline- and Gentamicin-resistant Bacteria. Appl Environ Microbiol. 2006;72(9):5870-6. PubMed PMID: 16957206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lettuce for human consumption collected in Costa Rica contains complex communities of culturable oxytetracycline- and gentamicin-resistant bacteria. AU - Rodríguez,César, AU - Lang,Lore, AU - Wang,Amy, AU - Altendorf,Karlheinz, AU - García,Fernando, AU - Lipski,André, PY - 2006/9/8/pubmed PY - 2006/11/10/medline PY - 2006/9/8/entrez SP - 5870 EP - 6 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 72 IS - 9 N2 - The present widespread use of antimicrobials in crop farming is based upon their successful application in human medicine. However, recent evidence suggests that the massive anthropogenic release of antimicrobials into the biosphere has selected for resistant bacteria and facilitated the transfer of resistance genes among them. This work deals with the examination of iceberg lettuce collected at 10 farms from two regions in Costa Rica. Farmers from nine sampling sites regularly apply commercial formulations containing gentamicin, oxytetracycline, streptomycin, or a combination of them without being able to indicate how often and how much of these products have been sprayed onto the crops. One organic farm was also investigated for comparative purposes. Oxytetracycline- and gentamicin-resistant bacteria were abundantly detected using selective enrichment cultures. Furthermore, colony mixtures from selective plates were characterized by chemotaxonomical and molecular fingerprinting methods. Both types of resistant communities accounted for a significant fraction of all culturable bacteria and included several resistance genes as well as factors for their potential horizontal transfer. Given the fact that lettuce is eaten raw, it may contribute to the dissemination of antimicrobial-resistant bacteria and/or their resistance genes from the environment to the microbial biota of the human intestine. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16957206/full_citation L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=16957206 DB - PRIME DP - Unbound Medicine ER -