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A comparison of modified methenamine silver and toluidine blue stains for the detection of Pneumocystis carinii in bronchoalveolar lavage specimens from immunosuppressed patients.
Acta Cytol. 1990 Jul-Aug; 34(4):511-6.AC

Abstract

Pneumonia due to Pneumocystis carinii is an increasingly frequent occurrence; a prompt, accurate diagnosis is important to successfully manage this infection. Methenamine silver and toluidine blue stainings of material recovered by bronchoalveolar lavage (BAL) have been the most widely employed techniques for detecting Pneumocystis organisms. The value of these two staining techniques for the detection of Pneumocystis organisms was prospectively compared in 220 BAL specimens obtained from 186 immunosuppressed individuals. The patients included those with disease-related and therapy-related immunosuppression; half of the BAL specimens came from organ transplant recipients. The results indicate that neither method is superior to the other and that both will correctly identify almost all Pneumocystis infections if a sufficient aliquot of BAL material is examined systematically by trained individuals. The toluidine blue method is somewhat simpler to perform, however. The reasons for discrepant results between the two staining methods were analyzed.

Authors+Show Affiliations

Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15261.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1695802

Citation

Paradis, I L., et al. "A Comparison of Modified Methenamine Silver and Toluidine Blue Stains for the Detection of Pneumocystis Carinii in Bronchoalveolar Lavage Specimens From Immunosuppressed Patients." Acta Cytologica, vol. 34, no. 4, 1990, pp. 511-6.
Paradis IL, Ross C, Dekker A, et al. A comparison of modified methenamine silver and toluidine blue stains for the detection of Pneumocystis carinii in bronchoalveolar lavage specimens from immunosuppressed patients. Acta Cytol. 1990;34(4):511-6.
Paradis, I. L., Ross, C., Dekker, A., & Dauber, J. (1990). A comparison of modified methenamine silver and toluidine blue stains for the detection of Pneumocystis carinii in bronchoalveolar lavage specimens from immunosuppressed patients. Acta Cytologica, 34(4), 511-6.
Paradis IL, et al. A Comparison of Modified Methenamine Silver and Toluidine Blue Stains for the Detection of Pneumocystis Carinii in Bronchoalveolar Lavage Specimens From Immunosuppressed Patients. Acta Cytol. 1990 Jul-Aug;34(4):511-6. PubMed PMID: 1695802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparison of modified methenamine silver and toluidine blue stains for the detection of Pneumocystis carinii in bronchoalveolar lavage specimens from immunosuppressed patients. AU - Paradis,I L, AU - Ross,C, AU - Dekker,A, AU - Dauber,J, PY - 1990/7/1/pubmed PY - 1990/7/1/medline PY - 1990/7/1/entrez SP - 511 EP - 6 JF - Acta cytologica JO - Acta Cytol VL - 34 IS - 4 N2 - Pneumonia due to Pneumocystis carinii is an increasingly frequent occurrence; a prompt, accurate diagnosis is important to successfully manage this infection. Methenamine silver and toluidine blue stainings of material recovered by bronchoalveolar lavage (BAL) have been the most widely employed techniques for detecting Pneumocystis organisms. The value of these two staining techniques for the detection of Pneumocystis organisms was prospectively compared in 220 BAL specimens obtained from 186 immunosuppressed individuals. The patients included those with disease-related and therapy-related immunosuppression; half of the BAL specimens came from organ transplant recipients. The results indicate that neither method is superior to the other and that both will correctly identify almost all Pneumocystis infections if a sufficient aliquot of BAL material is examined systematically by trained individuals. The toluidine blue method is somewhat simpler to perform, however. The reasons for discrepant results between the two staining methods were analyzed. SN - 0001-5547 UR - https://www.unboundmedicine.com/medline/citation/1695802/A_comparison_of_modified_methenamine_silver_and_toluidine_blue_stains_for_the_detection_of_Pneumocystis_carinii_in_bronchoalveolar_lavage_specimens_from_immunosuppressed_patients_ DB - PRIME DP - Unbound Medicine ER -