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Selenium-dependent pre- and posttranscriptional mechanisms are responsible for sexual dimorphic expression of selenoproteins in murine tissues.
Endocrinology 2006; 147(12):5883-92E

Abstract

Important enzymes for thyroid hormone metabolism, antioxidative defense, and intracellular redox control contain selenocysteine (Sec) in their active centers. Expression of these selenoproteins is tightly controlled, and a sex-specific phenotype is observed on disturbance of selenium (Se) transport in mice. Therefore, we analyzed Se concentrations and expression levels of several selenoproteins including type I iodothyronine deiodinase (Dio1) and glutathione peroxidase (GPx) isozymes in male and female mice. On regular lab chow, serum Se levels were comparable, but serum GPx3 activity was higher in females than males (1.3-fold). Selenoprotein P (SePP) mRNA levels were higher in livers (1.3-fold) and lower in kidneys (to 31%) in female compared with male mice. Orchidectomy alleviated the sex-specific differences in SePP mRNA amounts, indicating modulatory effects of androgens on SePP expression. Female mice expressed higher levels of Dio1 mRNA in kidney (2.6-fold) and liver (1.4-fold) in comparison with male mice. This sexual dimorphic expression of Dio1 mRNA was paralleled by increased Dio1 activity in female kidney (1.8-fold) but not in liver in which males expressed higher Dio1 activity (2.8-fold). Interestingly, Se deficiency decreased Dio1 activity more effectively in males than females, and resulting hepatic enzyme levels were then comparable between the sexes. At the same time, the sex-specific difference of Dio1 activity widened in kidney. Orchidectomy or estradiol treatment of ovariectomized females impacted stronger on renal than hepatic Dio1 expression. Thus, we conclude that Se-dependent posttranscriptional mechanisms are operational that affect either translational efficiency or Dio1 stability in a sex- and tissue-specific manner.

Authors+Show Affiliations

Institut fuer Experimentelle Endokrinologie, Charité-Universitaetsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16959840

Citation

Riese, Cornelia, et al. "Selenium-dependent Pre- and Posttranscriptional Mechanisms Are Responsible for Sexual Dimorphic Expression of Selenoproteins in Murine Tissues." Endocrinology, vol. 147, no. 12, 2006, pp. 5883-92.
Riese C, Michaelis M, Mentrup B, et al. Selenium-dependent pre- and posttranscriptional mechanisms are responsible for sexual dimorphic expression of selenoproteins in murine tissues. Endocrinology. 2006;147(12):5883-92.
Riese, C., Michaelis, M., Mentrup, B., Götz, F., Köhrle, J., Schweizer, U., & Schomburg, L. (2006). Selenium-dependent pre- and posttranscriptional mechanisms are responsible for sexual dimorphic expression of selenoproteins in murine tissues. Endocrinology, 147(12), pp. 5883-92.
Riese C, et al. Selenium-dependent Pre- and Posttranscriptional Mechanisms Are Responsible for Sexual Dimorphic Expression of Selenoproteins in Murine Tissues. Endocrinology. 2006;147(12):5883-92. PubMed PMID: 16959840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium-dependent pre- and posttranscriptional mechanisms are responsible for sexual dimorphic expression of selenoproteins in murine tissues. AU - Riese,Cornelia, AU - Michaelis,Marten, AU - Mentrup,Birgit, AU - Götz,Franziska, AU - Köhrle,Josef, AU - Schweizer,Ulrich, AU - Schomburg,Lutz, Y1 - 2006/09/07/ PY - 2006/9/9/pubmed PY - 2007/1/12/medline PY - 2006/9/9/entrez SP - 5883 EP - 92 JF - Endocrinology JO - Endocrinology VL - 147 IS - 12 N2 - Important enzymes for thyroid hormone metabolism, antioxidative defense, and intracellular redox control contain selenocysteine (Sec) in their active centers. Expression of these selenoproteins is tightly controlled, and a sex-specific phenotype is observed on disturbance of selenium (Se) transport in mice. Therefore, we analyzed Se concentrations and expression levels of several selenoproteins including type I iodothyronine deiodinase (Dio1) and glutathione peroxidase (GPx) isozymes in male and female mice. On regular lab chow, serum Se levels were comparable, but serum GPx3 activity was higher in females than males (1.3-fold). Selenoprotein P (SePP) mRNA levels were higher in livers (1.3-fold) and lower in kidneys (to 31%) in female compared with male mice. Orchidectomy alleviated the sex-specific differences in SePP mRNA amounts, indicating modulatory effects of androgens on SePP expression. Female mice expressed higher levels of Dio1 mRNA in kidney (2.6-fold) and liver (1.4-fold) in comparison with male mice. This sexual dimorphic expression of Dio1 mRNA was paralleled by increased Dio1 activity in female kidney (1.8-fold) but not in liver in which males expressed higher Dio1 activity (2.8-fold). Interestingly, Se deficiency decreased Dio1 activity more effectively in males than females, and resulting hepatic enzyme levels were then comparable between the sexes. At the same time, the sex-specific difference of Dio1 activity widened in kidney. Orchidectomy or estradiol treatment of ovariectomized females impacted stronger on renal than hepatic Dio1 expression. Thus, we conclude that Se-dependent posttranscriptional mechanisms are operational that affect either translational efficiency or Dio1 stability in a sex- and tissue-specific manner. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/16959840/Selenium_dependent_pre__and_posttranscriptional_mechanisms_are_responsible_for_sexual_dimorphic_expression_of_selenoproteins_in_murine_tissues_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2006-0689 DB - PRIME DP - Unbound Medicine ER -