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Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells.
Virology. 2007 Jan 05; 357(1):29-40.V

Abstract

The untranslated regions (UTRs) of the positive and negative strand RNAs of several viruses are major binding sites for cellular and viral proteins. Human La autoantigen is one of the cellular proteins that interacts with various positive strand RNA viral genomes including that of dengue virus (DEN) within the 5'- and 3'-UTRs of positive (+) and the 3'-UTR of negative strand (-) RNA, and with the nonstructural proteins NS3 and NS5, that form DEN replicase complex. Since DEN replicates in human and mosquito cells, some functional interactions have to be conserved in both hosts. In the present report, we demonstrate that mosquito La protein interacts with the 3'-UTRs of (+) and (-) polarity viral RNAs. The localization of La protein, examined by confocal microscopy, indicates that La protein is redistributed in DEN-infected cells. Furthermore, the presence of La protein in an in vitro replication system inhibited RNA synthesis in a dose-dependent manner, suggesting that La protein plays an important role in dengue virus replicative cycle.

Authors+Show Affiliations

Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, México City 03100, México.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16962153

Citation

Yocupicio-Monroy, Martha, et al. "Mosquito La Protein Binds to the 3' Untranslated Region of the Positive and Negative Polarity Dengue Virus RNAs and Relocates to the Cytoplasm of Infected Cells." Virology, vol. 357, no. 1, 2007, pp. 29-40.
Yocupicio-Monroy M, Padmanabhan R, Medina F, et al. Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells. Virology. 2007;357(1):29-40.
Yocupicio-Monroy, M., Padmanabhan, R., Medina, F., & del Angel, R. M. (2007). Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells. Virology, 357(1), 29-40.
Yocupicio-Monroy M, et al. Mosquito La Protein Binds to the 3' Untranslated Region of the Positive and Negative Polarity Dengue Virus RNAs and Relocates to the Cytoplasm of Infected Cells. Virology. 2007 Jan 5;357(1):29-40. PubMed PMID: 16962153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells. AU - Yocupicio-Monroy,Martha, AU - Padmanabhan,R, AU - Medina,Fernando, AU - del Angel,Rosa M, Y1 - 2006/09/07/ PY - 2006/02/14/received PY - 2006/03/13/revised PY - 2006/07/10/accepted PY - 2006/9/12/pubmed PY - 2007/1/20/medline PY - 2006/9/12/entrez SP - 29 EP - 40 JF - Virology JO - Virology VL - 357 IS - 1 N2 - The untranslated regions (UTRs) of the positive and negative strand RNAs of several viruses are major binding sites for cellular and viral proteins. Human La autoantigen is one of the cellular proteins that interacts with various positive strand RNA viral genomes including that of dengue virus (DEN) within the 5'- and 3'-UTRs of positive (+) and the 3'-UTR of negative strand (-) RNA, and with the nonstructural proteins NS3 and NS5, that form DEN replicase complex. Since DEN replicates in human and mosquito cells, some functional interactions have to be conserved in both hosts. In the present report, we demonstrate that mosquito La protein interacts with the 3'-UTRs of (+) and (-) polarity viral RNAs. The localization of La protein, examined by confocal microscopy, indicates that La protein is redistributed in DEN-infected cells. Furthermore, the presence of La protein in an in vitro replication system inhibited RNA synthesis in a dose-dependent manner, suggesting that La protein plays an important role in dengue virus replicative cycle. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/16962153/Mosquito_La_protein_binds_to_the_3'_untranslated_region_of_the_positive_and_negative_polarity_dengue_virus_RNAs_and_relocates_to_the_cytoplasm_of_infected_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(06)00454-5 DB - PRIME DP - Unbound Medicine ER -