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A miniaturized germanium-doped silicon dioxide-based surface plasmon resonance waveguide sensor for immunoassay detection.
Biosens Bioelectron. 2006 Oct 15; 22(4):519-25.BB

Abstract

A surface plasmon resonance (SPR) waveguide immunosensor fabricated by germanium-doped silicon dioxide was investigated in this study. The designed waveguide sensor consisted of a 10 microm SiO(2) substrate layer (n=1.469), a 10 microm Ge-SiO(2) channel guide (n=1.492) and a 50 nm gold film layer for immobilization of biomolecules and SPR signal detection. The resultant spectral signal was measured by a portable spectrophotometer, where the sensor was aligned by a custom-designed micro-positioner. The results of the glycerol calibration standards showed that the resonance wavelength shifted from 628 to 758 nm due to changes of refractive index from 1.36 to 1.418. Flow-through immunoassay on waveguide sensors also showed the interactions of protein A, monoclonal antibody (mAb ALV-J) and avian leucosis virus (ALVs) resulted in wavelength shifting of 4.17, 3.03 and 2.18 nm, respectively. The SPR dynamic interaction could also be demonstrated successfully in 4 min as the sensor was integrated with a lateral flow nitrocellulose strip. These results suggest that SPR detection could be carried out on designed waveguide sensor, and the integration of nitrocellulose strip for sample filtering and fluid carrier would facilitate applications in point-of-care portable system.

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Authors+Show Affiliations

Huang JG
Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
Lee CL
No affiliation info available
Lin HM
No affiliation info available
Chuang TL
No affiliation info available
Wang WS
No affiliation info available
Juang RH
No affiliation info available
Wang CH
No affiliation info available
Lee CK
No affiliation info available
Lin SM
No affiliation info available
Lin CW
No affiliation info available

MeSH

Biosensing TechniquesCollodionEquipment DesignEquipment Failure AnalysisFiber Optic TechnologyGermaniumImmunoassayMiniaturizationSilicon DioxideSurface Plasmon ResonanceSurface Properties

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16962763

Citation

Huang, Jhen-Gang, et al. "A Miniaturized Germanium-doped Silicon Dioxide-based Surface Plasmon Resonance Waveguide Sensor for Immunoassay Detection." Biosensors & Bioelectronics, vol. 22, no. 4, 2006, pp. 519-25.
Huang JG, Lee CL, Lin HM, et al. A miniaturized germanium-doped silicon dioxide-based surface plasmon resonance waveguide sensor for immunoassay detection. Biosens Bioelectron. 2006;22(4):519-25.
Huang, J. G., Lee, C. L., Lin, H. M., Chuang, T. L., Wang, W. S., Juang, R. H., Wang, C. H., Lee, C. K., Lin, S. M., & Lin, C. W. (2006). A miniaturized germanium-doped silicon dioxide-based surface plasmon resonance waveguide sensor for immunoassay detection. Biosensors & Bioelectronics, 22(4), 519-25.
Huang JG, et al. A Miniaturized Germanium-doped Silicon Dioxide-based Surface Plasmon Resonance Waveguide Sensor for Immunoassay Detection. Biosens Bioelectron. 2006 Oct 15;22(4):519-25. PubMed PMID: 16962763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A miniaturized germanium-doped silicon dioxide-based surface plasmon resonance waveguide sensor for immunoassay detection. AU - Huang,Jhen-Gang, AU - Lee,Chen-Lung, AU - Lin,Hsueh-Min, AU - Chuang,Tsung-Liang, AU - Wang,Way-Seen, AU - Juang,Rong-Huey, AU - Wang,Ching-Ho, AU - Lee,Chih Kung, AU - Lin,Shi-Ming, AU - Lin,Chii-Wann, Y1 - 2006/09/08/ PY - 2006/02/15/received PY - 2006/07/22/revised PY - 2006/07/25/accepted PY - 2006/9/12/pubmed PY - 2007/1/18/medline PY - 2006/9/12/entrez SP - 519 EP - 25 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 4 N2 - A surface plasmon resonance (SPR) waveguide immunosensor fabricated by germanium-doped silicon dioxide was investigated in this study. The designed waveguide sensor consisted of a 10 microm SiO(2) substrate layer (n=1.469), a 10 microm Ge-SiO(2) channel guide (n=1.492) and a 50 nm gold film layer for immobilization of biomolecules and SPR signal detection. The resultant spectral signal was measured by a portable spectrophotometer, where the sensor was aligned by a custom-designed micro-positioner. The results of the glycerol calibration standards showed that the resonance wavelength shifted from 628 to 758 nm due to changes of refractive index from 1.36 to 1.418. Flow-through immunoassay on waveguide sensors also showed the interactions of protein A, monoclonal antibody (mAb ALV-J) and avian leucosis virus (ALVs) resulted in wavelength shifting of 4.17, 3.03 and 2.18 nm, respectively. The SPR dynamic interaction could also be demonstrated successfully in 4 min as the sensor was integrated with a lateral flow nitrocellulose strip. These results suggest that SPR detection could be carried out on designed waveguide sensor, and the integration of nitrocellulose strip for sample filtering and fluid carrier would facilitate applications in point-of-care portable system. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16962763/A_miniaturized_germanium_doped_silicon_dioxide_based_surface_plasmon_resonance_waveguide_sensor_for_immunoassay_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00350-2 DB - PRIME DP - Unbound Medicine ER -