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Cannabinoid concentrations in hair from documented cannabis users.
Forensic Sci Int. 2007 Jul 04; 169(2-3):129-36.FS

Abstract

Fifty-three head hair specimens were collected from 38 males with a history of cannabis use documented by questionnaire, urinalysis and controlled, double blind administration of delta9-tetrahydrocannabinol (THC) in an institutional review board approved protocol. The subjects completed a questionnaire indicating daily cannabis use (N=18) or non-daily use, i.e. one to five cannabis cigarettes per week (N=20). Drug use was also documented by a positive cannabinoid urinalysis, a hair specimen was collected from each subject and they were admitted to a closed research unit. Additional hair specimens were collected following smoking of two 2.7% THC cigarettes (N=13) or multiple oral doses totaling 116 mg THC (N=2). Cannabinoid concentrations in all hair specimens were determined by ELISA and GCMSMS. Pre- and post-dose detection rates did not differ statistically, therefore, all 53 specimens were considered as one group for further comparisons. Nineteen specimens (36%) had no detectable THC or 11-nor-9-carboxy-THC (THCCOOH) at the GCMSMS limits of quantification (LOQ) of 1.0 and 0.1 pg/mg hair, respectively. Two specimens (3.8%) had measurable THC only, 14 (26%) THCCOOH only, and 18 (34%) both cannabinoids. Detection rates were significantly different (p<0.05, Fishers' exact test) between daily cannabis users (85%) and non-daily users (52%). There was no difference in detection rates between African-American and Caucasian subjects (p>0.3, Fisher's exact test). For specimens with detectable cannabinoids, concentrations ranged from 3.4 to >100 pg THC/mg and 0.10 to 7.3 pg THCCOOH/mg hair. THC and THCCOOH concentrations were positively correlated (r=0.38, p<0.01, Pearson's product moment correlation). Using an immunoassay cutoff concentration of 5 pg THC equiv./mg hair, 83% of specimens that screened positive were confirmed by GCMSMS at a cutoff concentration of 0.1 pg THCCOOH/mg hair.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, United States. mhuestis@irp.nida.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16963215

Citation

Huestis, Marilyn A., et al. "Cannabinoid Concentrations in Hair From Documented Cannabis Users." Forensic Science International, vol. 169, no. 2-3, 2007, pp. 129-36.
Huestis MA, Gustafson RA, Moolchan ET, et al. Cannabinoid concentrations in hair from documented cannabis users. Forensic Sci Int. 2007;169(2-3):129-36.
Huestis, M. A., Gustafson, R. A., Moolchan, E. T., Barnes, A., Bourland, J. A., Sweeney, S. A., Hayes, E. F., Carpenter, P. M., & Smith, M. L. (2007). Cannabinoid concentrations in hair from documented cannabis users. Forensic Science International, 169(2-3), 129-36.
Huestis MA, et al. Cannabinoid Concentrations in Hair From Documented Cannabis Users. Forensic Sci Int. 2007 Jul 4;169(2-3):129-36. PubMed PMID: 16963215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid concentrations in hair from documented cannabis users. AU - Huestis,Marilyn A, AU - Gustafson,Richard A, AU - Moolchan,Eric T, AU - Barnes,Allan, AU - Bourland,James A, AU - Sweeney,Stacy A, AU - Hayes,Eugene F, AU - Carpenter,Patrick M, AU - Smith,Michael L, Y1 - 2006/09/11/ PY - 2006/04/21/received PY - 2006/07/15/revised PY - 2006/08/14/accepted PY - 2006/9/12/pubmed PY - 2007/7/20/medline PY - 2006/9/12/entrez SP - 129 EP - 36 JF - Forensic science international JO - Forensic Sci Int VL - 169 IS - 2-3 N2 - Fifty-three head hair specimens were collected from 38 males with a history of cannabis use documented by questionnaire, urinalysis and controlled, double blind administration of delta9-tetrahydrocannabinol (THC) in an institutional review board approved protocol. The subjects completed a questionnaire indicating daily cannabis use (N=18) or non-daily use, i.e. one to five cannabis cigarettes per week (N=20). Drug use was also documented by a positive cannabinoid urinalysis, a hair specimen was collected from each subject and they were admitted to a closed research unit. Additional hair specimens were collected following smoking of two 2.7% THC cigarettes (N=13) or multiple oral doses totaling 116 mg THC (N=2). Cannabinoid concentrations in all hair specimens were determined by ELISA and GCMSMS. Pre- and post-dose detection rates did not differ statistically, therefore, all 53 specimens were considered as one group for further comparisons. Nineteen specimens (36%) had no detectable THC or 11-nor-9-carboxy-THC (THCCOOH) at the GCMSMS limits of quantification (LOQ) of 1.0 and 0.1 pg/mg hair, respectively. Two specimens (3.8%) had measurable THC only, 14 (26%) THCCOOH only, and 18 (34%) both cannabinoids. Detection rates were significantly different (p<0.05, Fishers' exact test) between daily cannabis users (85%) and non-daily users (52%). There was no difference in detection rates between African-American and Caucasian subjects (p>0.3, Fisher's exact test). For specimens with detectable cannabinoids, concentrations ranged from 3.4 to >100 pg THC/mg and 0.10 to 7.3 pg THCCOOH/mg hair. THC and THCCOOH concentrations were positively correlated (r=0.38, p<0.01, Pearson's product moment correlation). Using an immunoassay cutoff concentration of 5 pg THC equiv./mg hair, 83% of specimens that screened positive were confirmed by GCMSMS at a cutoff concentration of 0.1 pg THCCOOH/mg hair. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/16963215/Cannabinoid_concentrations_in_hair_from_documented_cannabis_users_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(06)00534-2 DB - PRIME DP - Unbound Medicine ER -