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Antioxidants and metallothionein levels in mercury-treated mice.
Cell Biol Toxicol. 2006 Nov; 22(6):429-38.CB

Abstract

Acute effects of mercury on mouse blood, kidneys, and liver were evaluated. Mice received a single dose of mercuric chloride (HgCl2, 4.6 mg/kg, subcutaneously) for three consecutive days. We investigated the possible beneficial effects of antioxidant therapy (N-acetylcysteine (NAC) and diphenyl diselenide (PhSe)2) compared with the sodium salt of 2,3-dimercapto-1-propanesulfonic acid (DMPS), an effective chelating agent in HgCl2 exposure in mice. We also verified whether metallothionein (MT) induction might be involved in a possible mechanism of protection against HgCl2 poisoning and whether different treatments would modify MT levels and other toxicological parameters. The results demonstrated that HgCl2 exposure significantly inhibited delta-aminolevulinate dehydratase (delta-ALA-D) activity in liver and only DMPS treatment prevented the inhibitory effect. Mercuric chloride caused an increase in renal non-protein thiol groups (NPSH) and none of the treatments modified renal NPSH levels. Urea concentration was increased after HgCl2 exposure. NAC plus (PhSe)2 was partially effective in protecting against the effects of mercury. DMPS and (PhSe)2 were effective in restoring the increment in urea concentration caused by mercury. Thiobarbituric acid-reactive substances (TBARS), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities and ascorbic acid levels were not modified after mercury exposure. Mercuric chloride poisoning caused an increase in hepatic and renal MT levels and antioxidant treatments did not modify this parameter. Our data indicated a lack of therapeutic effect of the antioxidants tested.

Authors+Show Affiliations

Departamento de Quimica, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16964587

Citation

Brandão, R, et al. "Antioxidants and Metallothionein Levels in Mercury-treated Mice." Cell Biology and Toxicology, vol. 22, no. 6, 2006, pp. 429-38.
Brandão R, Santos FW, Farina M, et al. Antioxidants and metallothionein levels in mercury-treated mice. Cell Biol Toxicol. 2006;22(6):429-38.
Brandão, R., Santos, F. W., Farina, M., Zeni, G., Bohrer, D., Rocha, J. B., & Nogueira, C. W. (2006). Antioxidants and metallothionein levels in mercury-treated mice. Cell Biology and Toxicology, 22(6), 429-38.
Brandão R, et al. Antioxidants and Metallothionein Levels in Mercury-treated Mice. Cell Biol Toxicol. 2006;22(6):429-38. PubMed PMID: 16964587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidants and metallothionein levels in mercury-treated mice. AU - Brandão,R, AU - Santos,F W, AU - Farina,M, AU - Zeni,G, AU - Bohrer,D, AU - Rocha,J B T, AU - Nogueira,C W, Y1 - 2006/09/11/ PY - 2006/05/02/received PY - 2006/07/11/accepted PY - 2006/9/12/pubmed PY - 2007/4/14/medline PY - 2006/9/12/entrez SP - 429 EP - 38 JF - Cell biology and toxicology JO - Cell Biol Toxicol VL - 22 IS - 6 N2 - Acute effects of mercury on mouse blood, kidneys, and liver were evaluated. Mice received a single dose of mercuric chloride (HgCl2, 4.6 mg/kg, subcutaneously) for three consecutive days. We investigated the possible beneficial effects of antioxidant therapy (N-acetylcysteine (NAC) and diphenyl diselenide (PhSe)2) compared with the sodium salt of 2,3-dimercapto-1-propanesulfonic acid (DMPS), an effective chelating agent in HgCl2 exposure in mice. We also verified whether metallothionein (MT) induction might be involved in a possible mechanism of protection against HgCl2 poisoning and whether different treatments would modify MT levels and other toxicological parameters. The results demonstrated that HgCl2 exposure significantly inhibited delta-aminolevulinate dehydratase (delta-ALA-D) activity in liver and only DMPS treatment prevented the inhibitory effect. Mercuric chloride caused an increase in renal non-protein thiol groups (NPSH) and none of the treatments modified renal NPSH levels. Urea concentration was increased after HgCl2 exposure. NAC plus (PhSe)2 was partially effective in protecting against the effects of mercury. DMPS and (PhSe)2 were effective in restoring the increment in urea concentration caused by mercury. Thiobarbituric acid-reactive substances (TBARS), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities and ascorbic acid levels were not modified after mercury exposure. Mercuric chloride poisoning caused an increase in hepatic and renal MT levels and antioxidant treatments did not modify this parameter. Our data indicated a lack of therapeutic effect of the antioxidants tested. SN - 0742-2091 UR - https://www.unboundmedicine.com/medline/citation/16964587/Antioxidants_and_metallothionein_levels_in_mercury_treated_mice_ L2 - https://doi.org/10.1007/s10565-006-0119-8 DB - PRIME DP - Unbound Medicine ER -