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The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine.
Cell Biol Toxicol. 2006 Nov; 22(6):439-53.CB

Abstract

We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural molecules for use in the treatment of prostate cancer owing to their involvement in apoptosis and cell cycle regulation.

Authors+Show Affiliations

Laboratory of Molecular Pathology, Institute of Pathology, Faculty of Medicine, Palacký University, Hnevotínská 3, 77515, Olomouc, Czech Republic. malikova.j@seznam.czNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16964588

Citation

Malíková, J, et al. "The Effect of Chelerythrine On Cell Growth, Apoptosis, and Cell Cycle in Human Normal and Cancer Cells in Comparison With Sanguinarine." Cell Biology and Toxicology, vol. 22, no. 6, 2006, pp. 439-53.
Malíková J, Zdarilová A, Hlobilková A, et al. The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine. Cell Biol Toxicol. 2006;22(6):439-53.
Malíková, J., Zdarilová, A., Hlobilková, A., & Ulrichová, J. (2006). The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine. Cell Biology and Toxicology, 22(6), 439-53.
Malíková J, et al. The Effect of Chelerythrine On Cell Growth, Apoptosis, and Cell Cycle in Human Normal and Cancer Cells in Comparison With Sanguinarine. Cell Biol Toxicol. 2006;22(6):439-53. PubMed PMID: 16964588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine. AU - Malíková,J, AU - Zdarilová,A, AU - Hlobilková,A, AU - Ulrichová,J, Y1 - 2006/09/11/ PY - 2006/03/29/received PY - 2006/07/18/accepted PY - 2006/9/12/pubmed PY - 2007/4/14/medline PY - 2006/9/12/entrez SP - 439 EP - 53 JF - Cell biology and toxicology JO - Cell Biol Toxicol VL - 22 IS - 6 N2 - We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural molecules for use in the treatment of prostate cancer owing to their involvement in apoptosis and cell cycle regulation. SN - 0742-2091 UR - https://www.unboundmedicine.com/medline/citation/16964588/The_effect_of_chelerythrine_on_cell_growth_apoptosis_and_cell_cycle_in_human_normal_and_cancer_cells_in_comparison_with_sanguinarine_ L2 - https://doi.org/10.1007/s10565-006-0109-x DB - PRIME DP - Unbound Medicine ER -