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Investigation of the mechanism of action of nonablative pulsed-dye laser therapy in photorejuvenation and inflammatory acne vulgaris.
Br J Dermatol 2006; 155(4):748-55BJ

Abstract

BACKGROUND

Nonablative lasers are widely used for treatment of wrinkles, atrophic scars and acne. These lasers stimulate dermal remodelling and collagen production, but the early molecular stimulus for this is unknown. The mechanism of nonablative lasers in inflammatory acne is variously suggested to be damage either to sebaceous glands or to Propionibacterium acnes. Their effects on cytokine production are unknown.

OBJECTIVES

To assess the in vivo effects of a short pulse duration nonablative pulsed-dye laser (NA-PDL) previously used for photorejuvenation and treatment of acne, on cytokine production, P. acnes colonization density and sebum excretion rate (SER).

METHODS

We examined the effect of NA-PDL (NliteV; Chromogenex Light Technologies, Llanelli, U.K.) on P. acnes colonization before and after laser therapy using a scrub-wash technique and culture at 0 and 24 h (n = 15), on SER using absorptive tape at 0, 2, 4, 8 and 12 weeks (n = 19) and on cytokine mRNA using reverse transcription-polymerase chain reaction from skin biopsies at 0, 3 and 24 h (n = 8). Results NA-PDL had no effect on P. acnes or SER. Transforming growth factor (TGF)-beta1 mRNA increased fivefold after 24 h and 15-fold in two subjects (P = 0.012).

CONCLUSIONS

TGF-beta is known to be a potent stimulus for neocollagenesis and a pivotal immunosuppressive cytokine which promotes inflammation resolution. Its upregulation by NA-PDL provides a possible unifying molecular mechanism linking stimulation of dermal remodelling in photorejuvenation with inhibition of inflammation in acne. Damage to P. acnes or sebaceous glands cannot explain the effect of this device in acne.

Authors+Show Affiliations

Department of Dermatology, Hammersmith Hospital, Imperial College, London W12 0NN, UK. edwardseaton@hotmail.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16965424

Citation

Seaton, E D., et al. "Investigation of the Mechanism of Action of Nonablative Pulsed-dye Laser Therapy in Photorejuvenation and Inflammatory Acne Vulgaris." The British Journal of Dermatology, vol. 155, no. 4, 2006, pp. 748-55.
Seaton ED, Mouser PE, Charakida A, et al. Investigation of the mechanism of action of nonablative pulsed-dye laser therapy in photorejuvenation and inflammatory acne vulgaris. Br J Dermatol. 2006;155(4):748-55.
Seaton, E. D., Mouser, P. E., Charakida, A., Alam, S., Seldon, P. M., Seldon, P. E., & Chu, A. C. (2006). Investigation of the mechanism of action of nonablative pulsed-dye laser therapy in photorejuvenation and inflammatory acne vulgaris. The British Journal of Dermatology, 155(4), pp. 748-55.
Seaton ED, et al. Investigation of the Mechanism of Action of Nonablative Pulsed-dye Laser Therapy in Photorejuvenation and Inflammatory Acne Vulgaris. Br J Dermatol. 2006;155(4):748-55. PubMed PMID: 16965424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of the mechanism of action of nonablative pulsed-dye laser therapy in photorejuvenation and inflammatory acne vulgaris. AU - Seaton,E D, AU - Mouser,P E, AU - Charakida,A, AU - Alam,S, AU - Seldon,P M, AU - Seldon,P E, AU - Chu,A C, PY - 2006/9/13/pubmed PY - 2007/3/3/medline PY - 2006/9/13/entrez SP - 748 EP - 55 JF - The British journal of dermatology JO - Br. J. Dermatol. VL - 155 IS - 4 N2 - BACKGROUND: Nonablative lasers are widely used for treatment of wrinkles, atrophic scars and acne. These lasers stimulate dermal remodelling and collagen production, but the early molecular stimulus for this is unknown. The mechanism of nonablative lasers in inflammatory acne is variously suggested to be damage either to sebaceous glands or to Propionibacterium acnes. Their effects on cytokine production are unknown. OBJECTIVES: To assess the in vivo effects of a short pulse duration nonablative pulsed-dye laser (NA-PDL) previously used for photorejuvenation and treatment of acne, on cytokine production, P. acnes colonization density and sebum excretion rate (SER). METHODS: We examined the effect of NA-PDL (NliteV; Chromogenex Light Technologies, Llanelli, U.K.) on P. acnes colonization before and after laser therapy using a scrub-wash technique and culture at 0 and 24 h (n = 15), on SER using absorptive tape at 0, 2, 4, 8 and 12 weeks (n = 19) and on cytokine mRNA using reverse transcription-polymerase chain reaction from skin biopsies at 0, 3 and 24 h (n = 8). Results NA-PDL had no effect on P. acnes or SER. Transforming growth factor (TGF)-beta1 mRNA increased fivefold after 24 h and 15-fold in two subjects (P = 0.012). CONCLUSIONS: TGF-beta is known to be a potent stimulus for neocollagenesis and a pivotal immunosuppressive cytokine which promotes inflammation resolution. Its upregulation by NA-PDL provides a possible unifying molecular mechanism linking stimulation of dermal remodelling in photorejuvenation with inhibition of inflammation in acne. Damage to P. acnes or sebaceous glands cannot explain the effect of this device in acne. SN - 0007-0963 UR - https://www.unboundmedicine.com/medline/citation/16965424/Investigation_of_the_mechanism_of_action_of_nonablative_pulsed_dye_laser_therapy_in_photorejuvenation_and_inflammatory_acne_vulgaris_ L2 - https://doi.org/10.1111/j.1365-2133.2006.07429.x DB - PRIME DP - Unbound Medicine ER -