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Rapid sample preparation method for LC-MS/MS or GC-MS analysis of acrylamide in various food matrices.
J Agric Food Chem. 2006 Sep 20; 54(19):7001-8.JA

Abstract

A fast and easy sample preparation procedure for analysis of acrylamide in various food matrices was developed and optimized. In its first step, deuterated acrylamide internal standard is added to 1 g of homogenized sample together with 5 mL of hexane, 10 mL of water, 10 mL of acetonitrile, 4 g of MgSO4, and 0.5 g of NaCl. Water facilitates the extraction of acrylamide; hexane serves for sample defatting; and the salt combination induces separation of water and acetonitrile layers and forces the majority of acrylamide into the acetonitrile layer. After vigorous shaking of the extraction mixture for 1 min and centrifugation, the upper hexane layer is discarded and a 1 mL aliquot of the acetonitrile extract is cleaned up by dispersive solid-phase extraction using 50 mg of primary secondary amine sorbent and 150 mg of anhydrous MgSO4. The final extract is analyzed either by liquid chromatography-tandem mass spectrometry or by gas chromatography-mass spectrometry (in positive chemical ionization mode) using the direct sample introduction technique for rugged large-volume injection.

Authors+Show Affiliations

Mastovska K
Eastern Regional Research Center, Agricultural Research Service, USDA, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.
Lehotay SJ
No affiliation info available

MeSH

AcrylamideChromatography, LiquidFood AnalysisGas Chromatography-Mass SpectrometryIndicators and ReagentsMagnesium SulfateMass SpectrometrySensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16968055

Citation

Mastovska, Katerina, and Steven J. Lehotay. "Rapid Sample Preparation Method for LC-MS/MS or GC-MS Analysis of Acrylamide in Various Food Matrices." Journal of Agricultural and Food Chemistry, vol. 54, no. 19, 2006, pp. 7001-8.
Mastovska K, Lehotay SJ. Rapid sample preparation method for LC-MS/MS or GC-MS analysis of acrylamide in various food matrices. J Agric Food Chem. 2006;54(19):7001-8.
Mastovska, K., & Lehotay, S. J. (2006). Rapid sample preparation method for LC-MS/MS or GC-MS analysis of acrylamide in various food matrices. Journal of Agricultural and Food Chemistry, 54(19), 7001-8.
Mastovska K, Lehotay SJ. Rapid Sample Preparation Method for LC-MS/MS or GC-MS Analysis of Acrylamide in Various Food Matrices. J Agric Food Chem. 2006 Sep 20;54(19):7001-8. PubMed PMID: 16968055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid sample preparation method for LC-MS/MS or GC-MS analysis of acrylamide in various food matrices. AU - Mastovska,Katerina, AU - Lehotay,Steven J, PY - 2006/9/14/pubmed PY - 2006/11/3/medline PY - 2006/9/14/entrez SP - 7001 EP - 8 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 19 N2 - A fast and easy sample preparation procedure for analysis of acrylamide in various food matrices was developed and optimized. In its first step, deuterated acrylamide internal standard is added to 1 g of homogenized sample together with 5 mL of hexane, 10 mL of water, 10 mL of acetonitrile, 4 g of MgSO4, and 0.5 g of NaCl. Water facilitates the extraction of acrylamide; hexane serves for sample defatting; and the salt combination induces separation of water and acetonitrile layers and forces the majority of acrylamide into the acetonitrile layer. After vigorous shaking of the extraction mixture for 1 min and centrifugation, the upper hexane layer is discarded and a 1 mL aliquot of the acetonitrile extract is cleaned up by dispersive solid-phase extraction using 50 mg of primary secondary amine sorbent and 150 mg of anhydrous MgSO4. The final extract is analyzed either by liquid chromatography-tandem mass spectrometry or by gas chromatography-mass spectrometry (in positive chemical ionization mode) using the direct sample introduction technique for rugged large-volume injection. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16968055/Rapid_sample_preparation_method_for_LC_MS/MS_or_GC_MS_analysis_of_acrylamide_in_various_food_matrices_ L2 - https://doi.org/10.1021/jf061330r DB - PRIME DP - Unbound Medicine ER -