TY - JOUR T1 - Development of analyses by high-performance liquid chromatography and liquid chromatography/tandem mass spectrometry of bilberry (Vaccinium myrtilus) anthocyanins in human plasma and urine. AU - Cooke,Darren N, AU - Thomasset,Sarah, AU - Boocock,David J, AU - Schwarz,Michael, AU - Winterhalter,Peter, AU - Steward,William P, AU - Gescher,Andreas J, AU - Marczylo,Timothy H, PY - 2006/9/14/pubmed PY - 2006/11/3/medline PY - 2006/9/14/entrez SP - 7009 EP - 13 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 19 N2 - Anthocyanins are potent antioxidants that may possess chronic disease preventive properties. Here, rapid, reliable, and reproducible solid-phase extraction, high-performance liquid chromatography (HPLC), and mass spectrometry techniques are described for the isolation, separation, and identification of anthocyanins in human plasma and urine. Recoveries of cyanidin-3-glucoside (C3G) were 91% from water, 71% from plasma, and 81% from urine. Intra- and interday variations for C3G extraction were 9 and 9.1% in plasma and 7.1 and 9.1% in urine and were less than 15% for all anthocyanins from a standardized bilberry extract (mirtoselect). Analysis of mirtoselect by HPLC with UV detection produced spectra with 15 peaks compatible with anthocyanin components found in mirtoselect within a total run time of 15 min. Chromatographic analysis of human urine obtained after an oral dose of mirtoselect yielded 19 anthocyanin peaks. Mass spectrometric analysis employing multiple reaction monitoring suggests the presence of unchanged anthocyanins and anthocyanidin glucuronide metabolites. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16968056/Development_of_analyses_by_high_performance_liquid_chromatography_and_liquid_chromatography/tandem_mass_spectrometry_of_bilberry__Vaccinium_myrtilus__anthocyanins_in_human_plasma_and_urine_ DB - PRIME DP - Unbound Medicine ER -