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High-performance liquid chromatography with photodiode array detection (HPLC-DAD)/HPLC-mass spectrometry (MS) profiling of anthocyanins from Andean Mashua Tubers (Tropaeolum tuberosum Ruíz and Pavón) and their contribution to the overall antioxidant activity.
J Agric Food Chem. 2006 Sep 20; 54(19):7089-97.JA

Abstract

Mashua (Tropaeolum tuberosum Ruíz and Pavón), an Andean tuber with high antioxidant activity, has sparked interest because of its traditional medicinal use. In this study, we evaluated the anthocyanin composition for three purple mashua genotypes and their contribution to the overall antioxidant activity of the tuber. Mashua anthocyanins, total phenolics, and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant activity ranged from 45.5 to 131.9 mg of cyanidin 3-glucoside equivalents/100 g fresh weight (FW), 174.9 to 275.5 mg of gallic acid equivalents/100 g of FW, and 16.2 to 45.7 micromol of Trolox equivalents/g of FW, respectively. The high-performance liquid chromatography with photodiode array detection (HPLC-DAD) and HPLC-electrospray ionization tandem mass spectrometry (ESI/MS-MS) profiles revealed the presence of 11 different anthocyanins. The two major pigments (56.4-73.0% total area range at 520 nm) were identified as delphinidin 3-glucoside-5-acetylrhamnoside and delphinidin 3-sophoroside-5-acetylrhamnoside. Other pigments were delphinidin 3-glucoside-5-rhamnoside, delphinidin 3-sophoroside-5-rhamnoside, delphinidin 3-glucoside, cyanidin 3-sophoroside, and cyanidin 3-sophoroside-5-rhamnoside. Cyanidin 3-glucoside and cyanidin 3-rutinoside were only found in two genotypes, while pelargonidin 3-sophoroside and pelargonidin 3-sophoroside-5-rhamnoside were only found in the third one. Anthocyanins from mashua were the major contributors to the total ABTS values for only one of the three genotypes, suggesting that other phenolics present are playing a major role in the antioxidant power of mashua tubers. Results from this study provide important information for the Nutraceutical and Functional Food Market for the use of mashua anthocyanins not only as a source of natural colorants but also as a source of phytonutrients.

Authors+Show Affiliations

Instituto de Biotecnología (IBT), Universidad Nacional Agraria La Molina (UNALM), Av. La Molina s/n, Lima, Peru.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16968067

Citation

Chirinos, Rosana, et al. "High-performance Liquid Chromatography With Photodiode Array Detection (HPLC-DAD)/HPLC-mass Spectrometry (MS) Profiling of Anthocyanins From Andean Mashua Tubers (Tropaeolum Tuberosum Ruíz and Pavón) and Their Contribution to the Overall Antioxidant Activity." Journal of Agricultural and Food Chemistry, vol. 54, no. 19, 2006, pp. 7089-97.
Chirinos R, Campos D, Betalleluz I, et al. High-performance liquid chromatography with photodiode array detection (HPLC-DAD)/HPLC-mass spectrometry (MS) profiling of anthocyanins from Andean Mashua Tubers (Tropaeolum tuberosum Ruíz and Pavón) and their contribution to the overall antioxidant activity. J Agric Food Chem. 2006;54(19):7089-97.
Chirinos, R., Campos, D., Betalleluz, I., Giusti, M. M., Schwartz, S. J., Tian, Q., Pedreschi, R., & Larondelle, Y. (2006). High-performance liquid chromatography with photodiode array detection (HPLC-DAD)/HPLC-mass spectrometry (MS) profiling of anthocyanins from Andean Mashua Tubers (Tropaeolum tuberosum Ruíz and Pavón) and their contribution to the overall antioxidant activity. Journal of Agricultural and Food Chemistry, 54(19), 7089-97.
Chirinos R, et al. High-performance Liquid Chromatography With Photodiode Array Detection (HPLC-DAD)/HPLC-mass Spectrometry (MS) Profiling of Anthocyanins From Andean Mashua Tubers (Tropaeolum Tuberosum Ruíz and Pavón) and Their Contribution to the Overall Antioxidant Activity. J Agric Food Chem. 2006 Sep 20;54(19):7089-97. PubMed PMID: 16968067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatography with photodiode array detection (HPLC-DAD)/HPLC-mass spectrometry (MS) profiling of anthocyanins from Andean Mashua Tubers (Tropaeolum tuberosum Ruíz and Pavón) and their contribution to the overall antioxidant activity. AU - Chirinos,Rosana, AU - Campos,David, AU - Betalleluz,Indira, AU - Giusti,M Monica, AU - Schwartz,Steven J, AU - Tian,Qingguo, AU - Pedreschi,Romina, AU - Larondelle,Yvan, PY - 2006/9/14/pubmed PY - 2006/11/3/medline PY - 2006/9/14/entrez SP - 7089 EP - 97 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 19 N2 - Mashua (Tropaeolum tuberosum Ruíz and Pavón), an Andean tuber with high antioxidant activity, has sparked interest because of its traditional medicinal use. In this study, we evaluated the anthocyanin composition for three purple mashua genotypes and their contribution to the overall antioxidant activity of the tuber. Mashua anthocyanins, total phenolics, and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant activity ranged from 45.5 to 131.9 mg of cyanidin 3-glucoside equivalents/100 g fresh weight (FW), 174.9 to 275.5 mg of gallic acid equivalents/100 g of FW, and 16.2 to 45.7 micromol of Trolox equivalents/g of FW, respectively. The high-performance liquid chromatography with photodiode array detection (HPLC-DAD) and HPLC-electrospray ionization tandem mass spectrometry (ESI/MS-MS) profiles revealed the presence of 11 different anthocyanins. The two major pigments (56.4-73.0% total area range at 520 nm) were identified as delphinidin 3-glucoside-5-acetylrhamnoside and delphinidin 3-sophoroside-5-acetylrhamnoside. Other pigments were delphinidin 3-glucoside-5-rhamnoside, delphinidin 3-sophoroside-5-rhamnoside, delphinidin 3-glucoside, cyanidin 3-sophoroside, and cyanidin 3-sophoroside-5-rhamnoside. Cyanidin 3-glucoside and cyanidin 3-rutinoside were only found in two genotypes, while pelargonidin 3-sophoroside and pelargonidin 3-sophoroside-5-rhamnoside were only found in the third one. Anthocyanins from mashua were the major contributors to the total ABTS values for only one of the three genotypes, suggesting that other phenolics present are playing a major role in the antioxidant power of mashua tubers. Results from this study provide important information for the Nutraceutical and Functional Food Market for the use of mashua anthocyanins not only as a source of natural colorants but also as a source of phytonutrients. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16968067/High_performance_liquid_chromatography_with_photodiode_array_detection__HPLC_DAD_/HPLC_mass_spectrometry__MS__profiling_of_anthocyanins_from_Andean_Mashua_Tubers__Tropaeolum_tuberosum_Ruíz_and_Pavón__and_their_contribution_to_the_overall_antioxidant_activity_ L2 - https://doi.org/10.1021/jf0614140 DB - PRIME DP - Unbound Medicine ER -