Tags

Type your tag names separated by a space and hit enter

Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.).
J Agric Food Chem. 2006 Sep 20; 54(19):7277-86.JA

Abstract

Four kinds of solvent extracts from three Chinese barley varieties (Ken-3, KA4B, and Gan-3) were used to examine the effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley through free radical scavenging activity, reducing power and metal chelating activity, and individual and total phenolic contents. Results showed that extraction solvent mixtures had significant impacts on antioxidant activity estimation, as well as different extraction capacity and selectivity for free phenolic compounds in barley. The highest DPPH* and ABTS*+ scavenging activities and reducing power were found in 80% acetone extracts, whereas the strongest *OH scavenging activity, O2*- scavenging activity, and metal chelating activity were found in 80% ethanol, 80% methanol, and water extracts, respectively. Additionally, 80% acetone showed the highest extraction capacity for (+)-catechin and ferulic, caffeic, vanillic, and p-coumaric acids, 80% methanol for (-)-epicatechin and syringic acid, and water for protocatechuic and gallic acids. Furthermore, correlations analysis revealed that TPC, reducing power, DPPH* and ABTS*+ scavenging activities were well positively correlated with each other (p < 0.01). Thus, for routine screening of barley varieties with higher antioxidant activity, 80% acetone was recommended to extract free phenolic compounds from barley. DPPH* scavenging activity and ABTS*+ scavenging activity or reducing power could be used to assess barley antioxidant activity.

Authors+Show Affiliations

Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16968094

Citation

Zhao, Haifeng, et al. "Effects of Extraction Solvent Mixtures On Antioxidant Activity Evaluation and Their Extraction Capacity and Selectivity for Free Phenolic Compounds in Barley (Hordeum Vulgare L.)." Journal of Agricultural and Food Chemistry, vol. 54, no. 19, 2006, pp. 7277-86.
Zhao H, Dong J, Lu J, et al. Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.). J Agric Food Chem. 2006;54(19):7277-86.
Zhao, H., Dong, J., Lu, J., Chen, J., Li, Y., Shan, L., Lin, Y., Fan, W., & Gu, G. (2006). Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.). Journal of Agricultural and Food Chemistry, 54(19), 7277-86.
Zhao H, et al. Effects of Extraction Solvent Mixtures On Antioxidant Activity Evaluation and Their Extraction Capacity and Selectivity for Free Phenolic Compounds in Barley (Hordeum Vulgare L.). J Agric Food Chem. 2006 Sep 20;54(19):7277-86. PubMed PMID: 16968094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.). AU - Zhao,Haifeng, AU - Dong,Jianjun, AU - Lu,Jian, AU - Chen,Jian, AU - Li,Yin, AU - Shan,Lianju, AU - Lin,Yan, AU - Fan,Wei, AU - Gu,Guoxian, PY - 2006/9/14/pubmed PY - 2006/11/3/medline PY - 2006/9/14/entrez SP - 7277 EP - 86 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 54 IS - 19 N2 - Four kinds of solvent extracts from three Chinese barley varieties (Ken-3, KA4B, and Gan-3) were used to examine the effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley through free radical scavenging activity, reducing power and metal chelating activity, and individual and total phenolic contents. Results showed that extraction solvent mixtures had significant impacts on antioxidant activity estimation, as well as different extraction capacity and selectivity for free phenolic compounds in barley. The highest DPPH* and ABTS*+ scavenging activities and reducing power were found in 80% acetone extracts, whereas the strongest *OH scavenging activity, O2*- scavenging activity, and metal chelating activity were found in 80% ethanol, 80% methanol, and water extracts, respectively. Additionally, 80% acetone showed the highest extraction capacity for (+)-catechin and ferulic, caffeic, vanillic, and p-coumaric acids, 80% methanol for (-)-epicatechin and syringic acid, and water for protocatechuic and gallic acids. Furthermore, correlations analysis revealed that TPC, reducing power, DPPH* and ABTS*+ scavenging activities were well positively correlated with each other (p < 0.01). Thus, for routine screening of barley varieties with higher antioxidant activity, 80% acetone was recommended to extract free phenolic compounds from barley. DPPH* scavenging activity and ABTS*+ scavenging activity or reducing power could be used to assess barley antioxidant activity. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16968094/Effects_of_extraction_solvent_mixtures_on_antioxidant_activity_evaluation_and_their_extraction_capacity_and_selectivity_for_free_phenolic_compounds_in_barley__Hordeum_vulgare_L___ L2 - https://dx.doi.org/10.1021/jf061087w DB - PRIME DP - Unbound Medicine ER -